A5080843233- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Tissue Engineering and Regenerative Medicine
- Diabetes and associated disorders
- Cell Adhesion Molecules Research
- Orthopedic Infections and Treatments
- Pancreatic function and diabetes
- Cellular Mechanics and Interactions
- Electrospun Nanofibers in Biomedical Applications
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Hydrogels: synthesis, properties, applications
- RNA Interference and Gene Delivery
- Connective tissue disorders research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Lymphoma Diagnosis and Treatment
- Bacterial biofilms and quorum sensing
- Cancer Cells and Metastasis
- Neuroendocrine Tumor Research Advances
- Antimicrobial Peptides and Activities
University of Oslo
2023-2024
Oslo University Hospital
2023-2024
Coalition for Epidemic Preparedness Innovations
2024
Georgia Institute of Technology
2018-2023
Cornell University
2016-2017
Ithaca College
2016
In-Q-Tel
2016
Significance Orthopedic implant infections require long-term antibiotic therapy and surgical debridement to successfully retain the implant; however, therapeutic failure can lead removal. Here an injectable PEG-based hydrogel that adheres exposed tissue fracture surfaces is engineered deliver antimicrobial enzyme lysostaphin infected, implant-fixed, mouse femoral fractures. Lysostaphin encapsulation within enhances stability while providing enhanced antibiofilm activity serving as a...
Abstract Stem cell therapies are limited by poor survival and engraftment. A hurdle to the use of materials for delivery is lack understanding material properties that govern transplanted stem functionality. Here, we show synthetic hydrogels presenting integrin-specific peptides enhance survival, persistence, osteo-reparative functions human bone marrow-derived mesenchymal cells (hMSCs) in murine defects. Integrin-specific regulate hMSC adhesion, paracrine signaling, osteoblastic...
Abstract A critical step in breast cancer progression is local tissue invasion, during which cells pass from the epithelial compartment to stromal compartment. We recently showed that malignant leader can promote invasion of otherwise non-invasive follower cells, but effects this induced-invasion phenomenon on cell phenotype remain unclear. Notably, process expose extracellular matrix (ECM), distinct ECM within normal microenvironment. Here, we used a 3D morphogenesis model were cultured...
Fiber alignment within tumor-mimetic engineered collagen matrices drives FAK- and Rac1-dependent cellular anisotropy that promotes protrusions along fibers suppresses off-axis to direct cell migration.
Use of BMP-2 and lysostaphin-loaded hydrogels simultaneously clears S. aureus infection repairs bone defects.
Local biomaterial-mediated delivery of PD-L1 induces alloislet graft survival and function in a murine model type 1 diabetes.
Allogeneic cellular immunotherapies hold promise for broad clinical implementation but face limitations due to potential rejection of donor cells by the host immune system. Silencing beta-2 microglobulin (B2M) expression is commonly employed evade T cell-mediated host, although absence B2M expected trigger missing-self responses natural killer (NK) cells. Here, we demonstrate that genetic deletion adhesion ligands CD54 and CD58 in B2M-deficient chimeric antigen receptor (CAR) multi-edited...
Hydrogel microparticles (microgels) are an attractive approach for therapeutic delivery because of their modularity, injectability, and enhanced integration with the host tissue. Multiple microgel fabrication strategies chemistries have been implemented, yet manipulation degradability its effect on in vivo tissue responses remains underexplored. Here, authors report a facile method to synthesize microgels crosslinked ester-containing junctions afford tunable degradation kinetics....
SUMMARY Allogeneic cell therapies hold promise for broad clinical implementation, but face limitations due to potential rejection by the recipient immune system. Silencing of beta-2-microglobulin ( B2M ) expression is commonly employed evade T cell-mediated rejection, although absence triggers missing-self responses natural killer (NK) cells. Here, we demonstrate that deletion adhesion ligands CD54 and CD58 on targets cells robustly dampens NK reactivity across all sub-populations. Genetic...
Human mesenchymal stromal cells (hMSCs) are a promising source for regenerative cell therapy. However, hMSC clinical use has been stymied by product variability across donors and manufacturing practices resulting in inconsistent outcomes. The inability to predict efficacy, or potency, is major limitation market penetration. Standard metrics of potency employ hMSCs third-party immune co-cultures, however, these assays face translational challenges due donor lack scalability. While surrogate...
<h3>Background</h3> ADAPT-NK cells are GMP-compliant, in vitro expanded adaptive NK cells, which naturally develop cytomegalovirus-positive individuals. This cell therapy platform harnesses the enhanced functional capabilities of and has shown superior anti-tumor properties due to expression activating NKG2C receptor, binds HLA-E liquid solid tumors, presence a single-KIR making them suited for transfer across HLA barriers. To further improve therapeutic potential we investigated genetic...
<h3>Background</h3> Differences in the persistence and anti-tumor responses of various NK cell subsets represent key hurdles to implementation cell-based cancer immunotherapies. Our lab has recently developed a protocol for specific expansion adaptive subpopulation.<sup>1</sup> Due their unique KIR repertoire with dominant expression one single KIR, cells deliver strong, predictable missing-self response an allogeneic, HLA-C/KIR mismatch setting. However, allorejection may significantly...
On-Chip Technologies In article number 2101995, using 3D encapsulation and microfluidics, Andrés J. García co-workers engineer a high-throughput on-chip human mesenchymal stromal cell (MSC) potency assay with improved functional prediction compared to traditional 2D assays. Further results demonstrate in vivo secretory recapitulation of MSCs cultures.