A5049976699- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Hematopoietic Stem Cell Transplantation
- Diabetes Management and Research
- Immune Response and Inflammation
- Xenotransplantation and immune response
- Transplantation: Methods and Outcomes
- Cancer Research and Treatments
- Renal Transplantation Outcomes and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- Organ Transplantation Techniques and Outcomes
- Cell death mechanisms and regulation
- Tissue Engineering and Regenerative Medicine
- Cannabis and Cannabinoid Research
- Cervical Cancer and HPV Research
- Viral Infections and Immunology Research
University of Missouri Health System
2020-2024
University of Missouri
2020-2024
University of Louisville
2014-2024
Center of Molecular Immunology (Cuba)
2023
Baxter (United States)
2004-2023
Institute of Child Health
2023
MGH Institute of Health Professions
2023
Cell Biotech (South Korea)
2023
Biocat
2023
University of Louisville Hospital
2003-2019
Abstract Sleep fragmentation (SF) is a highly prevalent condition and hallmark of sleep apnea, that has been associated with increased cancer incidence mortality. In this study, we examined the hypothesis promotes tumor growth progression through proinflammatory TLR4 signaling. design, compared mice were exposed to one week before engraftment syngeneic TC1 or LL3 cells analysis four weeks later. We also host contributions use genetically deficient in its effector molecules MYD88 TRIF. found...
Islet transplantation is a promising alternative therapy for insulin-dependent patients, with the potential to eliminate life-threatening hypoglycemic episodes and secondary complications of long-term diabetes. However, widespread application this has been limited by inadequate graft function longevity, in part due loss up 60% hostile intrahepatic transplant site. We report proteolytically degradable synthetic hydrogel, functionalized vasculogenic factors localized delivery, engineered...
Exosomes show potential as ideal vehicles for drug delivery because of their natural role in transferring biological cargo between cells. However, current methods to engineer exosomes without negatively impacting function remain challenging. Manipulating exosome-secreting cells is complex and time-consuming, while direct functionalization exosome surface proteins suffers from low specificity efficiency. We demonstrate a rapid, versatile, scalable method with oligonucleotide tethers enable...
Local biomaterial-mediated delivery of PD-L1 induces alloislet graft survival and function in a murine model type 1 diabetes.
Islet transplantation to treat insulin-dependent diabetes is greatly limited by the need for maintenance immunosuppression. We report a strategy through which cotransplantation of allogeneic islets and streptavidin (SA)–FasL–presenting microgels omentum under transient rapamycin monotherapy resulted in robust glycemic control, sustained C-peptide levels, graft survival diabetic nonhuman primates >6 months. Surgical extraction prompt hyperglycemia. In contrast, animals receiving without...
Naturally occurring CD4(+)CD25(+)FoxP3(+) T regulatory (Treg) cells require three distinct signals transduced via TCR, CD28, and IL-2R for their development maintenance. These requirements served as the basis several recently developed ex vivo expansion protocols that relied on use of solid support-bound Abs to CD3 CD28 in presence high dose IL-2. We report this study Treg up-regulate expression inducible costimulatory receptor 4-1BB response IL-2, stimulation using a novel form ligand...
Abstract Tumors use a complex set of direct and indirect mechanisms to evade the immune system. Naturally arising CD4+CD25+FoxP3+ T regulatory (Treg) cells have been implicated recently in tumor escape mechanism, but relative contribution these overall progression compared with other evasion remains be elucidated. Using A20 B cell lymphoma as transplantable model, we demonstrate that this employs multiple (expression immunoinhibitory molecule PD-L1, IDO, IL-10, lack expression CD80...
The transplanting islets to the liver approach suffers from an immediate posttransplant loss of more than 50%, progressive graft dysfunction over time, and precludes recovery grafts should there be serious complications such as development teratomas with that are stem cell-derived (SC-islets). omentum features attractive extrahepatic alternative site for clinical islet transplantation. We explore in which allogeneic transplanted onto omentum, is bioengineered a plasma-thrombin biodegradable...
Abstract Vaccines represent an attractive treatment modality for the management of cancer primarily because their specificity and generation immunologic memory important controlling recurrences. However, efficacy therapeutic vaccines may require formulations that not only generate effective immune responses but also overcome evasion mechanisms employed by progressing tumor. Costimulatory molecules play critical roles in modulating innate, adaptive, regulatory immunity have potential to serve...
Abstract Allogeneic islet transplantation is an important therapeutic approach for the treatment of type 1 diabetes. Clinical application this approach, however, severely curtailed by allograft rejection primarily initiated pathogenic effector T cells regardless chronic use immunosuppression. Given role Fas-mediated signaling in regulating cell responses, we tested if pancreatic islets can be engineered ex vivo to display on their surface apoptotic form Fas ligand protein chimeric with...
Long-term survival of heart transplants is hampered by chronic rejection (CR). Studies indicate the involvement host macrophages in development CR; however, precise role these cells CR unclear. Thus, it important to develop noninvasive techniques serially monitor movement and distribution recipient cardiac allograft vivo.We have employed a rat heterotopic working-heart model for magnetic resonance imaging experiment. Twenty-one (PVG.1U-->PVG.R8) 9 isograft (PVG.R8-->PVG.R8) transplantations...
Therapeutic subunit vaccines based on tumor-associated antigens (TAA) represent an attractive approach for the treatment of cancer. However, poor immunogenicity TAAs requires potent adjuvants therapeutic efficacy. We recently proposed tumor necrosis factor family costimulatory ligands as potential and, hence, generated a soluble form 4-1BBL chimeric with streptavidin (SA-4-1BBL) that has pleiotropic effects cells innate, adaptive, and regulatory immunity. herein tested whether these can...
Allogeneic islet transplantation is limited by adverse effects of chronic immunosuppression used to control rejection. The programmed cell death 1 pathway as an important immune checkpoint has the potential obviate need for immunosuppression. We generated oligomeric form ligand chimeric with core streptavidin (SA-PDL1) that inhibited T effector response alloantigens and converted conventional cells into CD4+Foxp3+ regulatory cells. SA-PDL1 protein was effectively displayed on surface...
We studied the rejection of cardiac allografts in a rat strain combination (PVG.R8 to PVG.1U) disparate for single class I MHC antigen (RT1.Aa) test extent by which this molecule is recognized as peptides association with recipient molecules during graft and contribution recognition process reaction. Three synthetic that correspond portions alpha-helices alpha 1 (P1, P2) 2 (P3) domains donor RT1.Aa were used study. Splenocytes from heart allograft recipients at responded proliferation assay...