A5008408610- Retinoids in leukemia and cellular processes
- Atherosclerosis and Cardiovascular Diseases
- Circadian rhythm and melatonin
- interferon and immune responses
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Antioxidant Activity and Oxidative Stress
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Inflammasome and immune disorders
- Inflammation biomarkers and pathways
- Cancer, Lipids, and Metabolism
- Neuroscience of respiration and sleep
- Cytokine Signaling Pathways and Interactions
- Protein Kinase Regulation and GTPase Signaling
- MicroRNA in disease regulation
- Galectins and Cancer Biology
- Obstructive Sleep Apnea Research
- Cancer Research and Treatments
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Dietary Effects on Health
- Heme Oxygenase-1 and Carbon Monoxide
- Cardiovascular and Diving-Related Complications
First Affiliated Hospital of Anhui Medical University
2012-2021
Anhui Medical University
2012-2021
Anhui University
2014-2015
Heritage Christian University
2005
This article reports on a noninvasive approach in detecting and following-up individuals who are at-risk or have an existing COVID-19 infection, with potential ability to serve as epidemic control tool. The proposed method uses developed breath device composed of nanomaterial-based hybrid sensor array multiplexed detection capabilities that can detect disease-specific biomarkers from exhaled breath, thus enabling rapid accurate diagnosis. An exploratory clinical study this was examined...
Melatonin, an indolamine produced and secreted predominately by the pineal gland, exhibits a variety of physiological functions, possesses antioxidant antitumor properties. But, mechanisms for anti-cancer effects are unknown. The present study explored melatonin on migration human lung adenocarcinoma A549 cells its mechanism.MTT assay was employed to measure viability treated with different concentrations melatonin. effect analyzed wound healing assay. Occludin location observed...
AIM:To investigate the expression frequency of endocan in colorectal cancer and analyze relationship between clinical parameters to study role carcinogenesis.METHODS: Expression 72 tumor tissue samples as well 27 normal mucous membrane was analyzed using situ hybridization, immunohistochemistry on microarray, Western blot reverse-transcript polymerase chain reaction (RT-PCR).RESULTS: The higher colon rectum than cancerous (mRNA = 92.6%,protein 36%), lower 70.4%,protein 36.1%).No correlation...
The pineal hormone, melatonin (MLT), has been shown to have therapeutic effects in patients with gastric cancer; however, the mechanisms for anti-cancer are unknown. We investigated of on cell proliferation, apoptosis, colony formation and migration adenocarcinoma line, SGC7901, using MTT assay, Hoechst 33258 staining, flow cytometry, western blot, caspase-3 activity soft agar scratch-wound assay. Our results showed that could inhibit efficiency, it promoted apoptosis SGC7901 cells. findings...
Background: Melatonin, which is mainly produced by the pineal gland, has a good inhibitory effect on cell growth of multiple cancer types. However, underlying molecular mechanisms anti-tumor activity for colon have not been fully elucidated. In this study, we investigated effects melatonin migration in human RKO cells and potential mechanisms. Materials Methods: The viability was MTT assay after treatment with melatonin, SB203580 (p38 inhibitor) phorbol 12-myristate 13-acetate (PMA, MAPK...
The development of atherosclerosis (AS) is a multifactorial process in which elevated plasma cholesterol levels play central role. As new class players involved AS, the regulation and function microRNAs (miR) response to AS remain poorly understood. This study analyzed effects miR-1 (antagomir mimic) on endothelial permeability myosin light chain kinase (MLCK) expression activity artery wall apoE knock-out mice after feeding them high-cholesterol diet. Further, we tested determine whether...
Krüppel-like factor 4 (KLF4) is an epithelial-specific transcription primarily expressed in the gastrointestinal tract that mediates growth arrest colonic epithelium. We tried to find whether KLF4 expression associated with progression and differentiation of colorectal cancer.We detected 109 specimens (40 normal appearing mucosa, 7 adenomas, 62 carcinomas) by immunohistochemistry using a tissue microarray. Western blot RT-PCR analyses were also performed.The upregulation carcinoma was...
Acute lung injury (ALI) is characterized by acute inflammation and apoptosis of alveolar epithelial cells (AECs) with a high morbidity mortality. Procyanidin B2 (PCB2) naturally occurring flavonoid anti-inflammatory activity. Our previous study demonstrated that PCB2 inhibited NLRP3 inflammasome signaling ameliorated paraquat-induced ALI in rat, indicating the protective role PCB2. As lipopolysaccharide (LPS) induced cell dysfunction, we continued to evaluate effects using LPS-treated human...
All-trans-retinoic acid (ATRA) inhibits the invasive and metastatic potentials of various cancer cells. However, underlying mechanism is unclear. Here, we demonstrate that ATRA inhibited colorectal cells RKO (human colon adenocarcinoma cell) migration by downregulating cell movement increasing adhesion. expression activation myosin light chain kinase (MLCK) in cells, while level MLC phosphatase (MLCP) had no change treated with or without ATRA. The activity was also exposed to Intriguingly,...
Protein tyrosine-phosphatases (PTPs) play important roles in various biological processes. Deregulation PTP function has been implicated carcinogenesis and tumour progression many cancer types. However, the role of protein tyrosine phosphatase receptor type B (PTPRB) non-small-cell lung (NSCLC) tumorigenesis not investigated. Lentiviral vector expressing PTPRB cDNA or shRNA was infected into A549 H1299 cell lines, followed by proliferation, colony formation, soft agar invasion assays....
Melatonin, the main secretary product of pineal gland, is potentially effective in prevention a number diseases which free radical processes are involved. The development hypercholesterolemia multifactorial process elevated oxidized low-density lipoprotein (ox-LDL) levels play central role. purpose this study was to test whether melatonin prevents ox-LDL-induced increase myosin light chain kinase (MLCK) activation and expression human umbilical vein endothelial cells (HUVECs) through...
Objective: The present study was designed to investigate the probable mechanisms of synthetic retinoid 4-amino-2-tri-fluoromethyl-phenyl ester (ATPR) inhibition proliferation and migration A549 human lung carcinoma cells. Materials Methods: After cells were treated with different concentrations ATPR or all-trans retinoic acid (ATRA) for 72 h, scratch-wound assays performed assess migration. Immunofluorescence used determine distribution CAV1 <TEX>$RXR{\alpha}$</TEX>, while expression CAV1,...
Endothelial dysfunction (ED) and hyperpermeability are considered as the initiating steps in early atherosclerosis. Phosphorylation of myosin light chain (MLC) is key to cause vascular via endothelial cell contraction. However, it unclear whether MLC phosphorylation can also regulate balance between contraction relaxation cells, thereby affecting endothelium-dependent diastolic function leading ED. The present study investigated relationships ED underlying mechanisms. Twenty-four male New...
Aim: To observe the effects of a novel all-trans retinoid acid (ATRA) derivative, N-(3-trifluoromethyl-phenyl)-retinamide (ATPR), on lung adenocarcinoma A549 cells and to explore potential mechanism ATPR inhibiting cell migration. Materials Methods: The cytotoxicity ATRA was assessed using MTT assay. Wound healing assays were used analyze influences ATRA, ATPR, ML-7 (a highly selective inhibitor myosin light chain kinase (MLCK)), PMA (an activator MAPKs) PD98059 ERK1/2) migration cells....
Hypoxia induces vigorous growth and a higher malignant phenotype in solid tumors. Hyperoxic treatment using hyperbaric oxygen (HBO) has previously been shown as highly effective method to attenuate hypoxia. We aimed investigate the effect of HBO on hypoxia-induced malignancy lung cancer cells. Cobalt chloride (CoCl2) was used induce chemical hypoxia cell line A549. Hypoxic inducible factor-1α (HIF-1α) expression, lactate dehydrogenase (LDH) activity, migration invasion capacity, expression...
Aims: To explore the effect and probable mechanism of a synthetic retinoid 4-amino-2-tri-fluoromethylphenyl ester (ATPR) on apoptosis MDA-MB-231 breast cancer cells. Materials Methods: MTT assays were performed to measure proliferation cells treated with different concentrations all-trans retinoic acid (ATRA) ATPR. Morphologic changes observed by microscopy. The rates cell cycling ATRA or ATPR assessed using flow cytometry analysis. Expression receptor phosphorylation ERK, JNK, p38 proteins...
The present study aimed to investigate the effects of nucleotide-binding domain leucine-rich repeat protein (NLRP)1/NLRP3 inflammasome pathways on latent viral infection respiratory tract. A total 55 BALB/c mice were assigned control, bleomycin (BLM)‑treated, murine cytomegalovirus (MCMV), MCMV+BLM and MCMV+BLM+CD4+ T‑cell groups. loads detected in salivary glands, kidney, liver lung tissues via polymerase chain reaction (PCR). weight, coefficient hydroxyproline (HYP) detected. HE Masson...