A5050111921- 3D Printing in Biomedical Research
- Analytical Chemistry and Sensors
- Barrier Structure and Function Studies
- Microfluidic and Bio-sensing Technologies
- Metal and Thin Film Mechanics
- Diamond and Carbon-based Materials Research
- Semiconductor materials and devices
- Advanced Sensor and Energy Harvesting Materials
- Electron and X-Ray Spectroscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Electrical and Bioimpedance Tomography
- Advanced Biosensing Techniques and Applications
- Liver physiology and pathology
- Force Microscopy Techniques and Applications
- Neuroscience and Neural Engineering
- Luminescence and Fluorescent Materials
- Spectroscopy Techniques in Biomedical and Chemical Research
- bioluminescence and chemiluminescence research
- NMR spectroscopy and applications
- Calcium Carbonate Crystallization and Inhibition
- Nanowire Synthesis and Applications
- GNSS positioning and interference
- Non-Invasive Vital Sign Monitoring
- Advanced Chemical Physics Studies
- Silicon Nanostructures and Photoluminescence
Institute for Bioengineering of Catalonia
2022-2023
Harvard University
2022
Brigham and Women's Hospital
2022
Institut de Microelectrònica de Barcelona
2013-2022
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2014-2018
Universitat Autònoma de Barcelona
2015-2018
Digital Video (Italy)
2018
Bioengineering Center
2017
Bradley University
2005-2010
Universitat Politècnica de Catalunya
2006
Abstract Tight junctions form selectively permeable seals across the paracellular space. Both barrier function and selective permeability have been attributed to members of claudin protein family, which can be categorized as pore-forming or barrier-forming. Here, we show that claudin-4, a prototypic barrier-forming claudin, reduces by previously unrecognized mechanism. Claudin-4 knockout overexpression has minimal effects on tight junction in absence claudins. However, claudin-4 inhibits...
This work presents a new microfluidic chip to facilitate multicellular interactions and cell barrier function monitoring in real time.
Transepithelial electrical resistance (TEER) measurements are regularly used in vitro models to quantitatively evaluate the cell barrier function.Although it would be expected that TEER values obtained with same type and experimental setup were comparable, reported literature show a large dispersion for unclear reasons.This work highlights possible error widely formula calculate TEER, which may erroneously assumed entire culture area contributes equally measurement.In this study, we have...
Abstract We demonstrate an integrated optical pressure sensing platform for multiplexed optofluidics applications. The consists in array of elastomeric on-side nanostructured membranes -effectively 2D photonic crystal- which present colour shifts response to mechanical stress that alter their nanostructure characteristical dimensions, pitch or orientation. are prepared by a simple and cost-effective method based on the infiltration colloidal crystal (CPC) with PDMS integration microfluidic...
Abstract Organ‐on‐a‐chip (OOC) devices bring innovative disease modeling and drug discovery approaches by providing biomimetic models of tissues organs in vitro combined with biosensors. Miniaturized biosensor systems tissue biofabrication techniques allow to create multiple on a chip highly controlling the experimental variables for high‐content screening applications. In this work, multi‐OOC integrated platform composed skeletal muscle pancreatic cells is fabricated study impact exercise...
We describe a unique, versatile bioreactor consisting of two plates and modified commercial porous membrane suitable for in vitro analysis the liver sinusoid. The modular allows i) excellent control cell seeding process; ii) culture under controlled shear stress stimulus, and; iii) individual each type upon completion experiment. advantages detailed here are derived from modification with an elastomeric wall specifically moulded order to define area, act as gasket that will fit into...
We report the formation of highly organized fibrous networks on ∼50 nm thick oligomeric films hydrolyzed γ-(aminopropyl)triethoxysilane (γ-APS) adsorbed Na+-containing substrates. The growth these nanostructures is dependent surface segregation Na+ ions and exposure to ambient conditions. Using an atomic force microscope (AFM), we have followed characteristics in a time-resolved manner. fibers, which grow 2-dimensionally, uniform height approximately 3 widths varying from few nanometers...
Immunoassays show great potential for the detection of low levels cytokines, due to their high sensitivity and excellent specificity. There is a particular demand biosensors that enable both high-throughput screening continuous monitoring clinically relevant cytokines such as interleukin-6 (IL-6) tumor necrosis factor-α (TNFα). To this end, we here introduce novel bioluminescent immunoassay based on ratiometric plug-and-play immunodiagnostics (RAPPID) platform, with an improved intrinsic...
Type I diabetes mellitus is characterised by the destruction of insulin producing beta cells within pancreas immune system. After success Edmonton protocol, islet transplantation has shown to be a promising therapy, but with Achilles´ heel need using immunosuppressive drugs. Currently, cell encapsulation technology represents real alternative protect transplanted islets from host´s attack. Although preliminary in vitro studies encapsulated have been traditionally performed under static...
Abstract Precision medicine is starting to incorporate functional assays evaluate anticancer agents on patient-isolated tissues or cells select for the most effective. Among these new technologies, dynamic BH3 profiling (DBP) has emerged and extensively been used predict treatment efficacy in different types of cancer. DBP uses synthetic peptides measure early apoptotic events (‘priming’) anticipate therapy-induced cell death leading tumor elimination. This predictive assay presents multiple...
In this work, we present the use of interdigitated electrodes (IDEs) for performing electrical impedance spectroscopy (EIS) measurements to monitor a microfluidic blood brain barrier model. particular, an electrode configuration which would not impair optical visualization cell culture is proposed. Numerical studies have been performed evaluate sensitivity proposed tetrapolar along in given chamber geometry. The system has validated using home-made cyclo olefin polymer (COP) bioreactor and...
In [1] a general overview of the PAU system is provided. This work describes in more detail implementation polarimetric and pseudo-correlation radiometer (PAU-RAD) that measures four Stokes parameters.
Abstract Transepithelial electrical measurements in the renal tubule have provided a better understanding of how kidney regulates electrolyte and water homeostasis through reabsorption molecules ions (e.g., H 2 O NaCl). While experiments measurement techniques using native tissue are difficult to prepare reproduce, cell cultures conducted largely with Ussing chamber lack effect fluid shear stress which is key physiological stimulus tubule. To overcome these limitations, we present modular...
Self-assembly of KHCO3 fibers is observed when glassy oligomerized films poly[(aminopropyl)siloxane] containing K+ ions, denoted K+/poly-APS, are exposed to CO2 and H2O. The crystalline, narrow (0.4−3 μm diam), high aspect ratio (up at least 300), and, on the basis Raman spectroscopy, dominated by KHCO3. contain fluid that aqueous potassium formate (KOOCH). A multistep phenomenological model proposed account for self-assembly.
We developed a microfluidic device compatible with hyperpolarization-enhanced MR spectroscopic imaging. It measures up to 8 chemical reactions simultaneously single hyperpolarized sample.
We present the fabrication of nanomechanical surface stress based transducers by using nowadays knowns as smart materials, to achieve a power-free array sensors that change their reflective color depending on induced each sensor. Nanocomposite materials elastomeric polymers and ordered nanoparticles embedded inside polymer were chosen for process. These composite besides being cheap easily fabricated in mass production, mechanochromic behavior producing material when applying deformation...
Nanostructured materials provide an outstanding opportunity to both stimulate and measure cellular processes. In the context of tight junctions, it was previously reported that transient application a nanotopographic surface over apical brush border membrane epithelial monolayers triggers redistribution ZO-1, claudins, F-actin increases paracellular macromolecular flux. excitable tissues, nanomaterials have been used apply electrical signals, such action potentials. As first step towards...