Introduction Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that can affect substantially any organ in the body. One of its most severe manifestations nephritis. Hereditary C1q deficiency strongly related to SLE but there are very few and inconsistent studies exploring single nucleotide polymorphisms (SNPs) gene cluster relation pathogenesis SLE. In present study we evaluated possible association variants complement with susceptibility nephritis Bulgarian population,...

A microfluidic sensor was studied for the photometric detection of active chlorine, total and pH in swimming pool samples. The consisted a four-layer borosilicate glass chip, containing microchannel network 2.2 mm path length, 1.7 mL optical cell. chip optimised to measure bleaching methyl orange spectral changes phenol red quantitative chlorine (active total) measurements that were suited monitoring. Reagent consumption (60 per measurement) minimised allow maintenance-free operation over...

IntroductionComplement system overactivation is pivotal in LN pathophysiology. As anti-C3 autoantibodies play a significant role pathophysiology, we explored them as disease activity biomarkers and compared to the ones against homologous protein C4.MethodsWe investigated presence of anti-C4 IgG cohort (n=85 patients) monitored their changes over time. We correlated autoantibody with clinical parameters. conducted cross-sectional longitudinal analyses (n=295 samples, 8 years follow-up)...

Data on the prevalence of macroprolactinemia in patients with prolactinomas is quite limited as presence high-molecular prolactin forms suspected mainly subjects mild hyperprolactinemia and negative pituitary imaging. The main objective this observational case-control study was to assess clinical significance among prolactinomas. population consisted 239 subjects: 131 prolactinoma 108 sex-, age- ethnicity– matched healthy controls. Macroprolactinemia defined by a PRL recovery after PEG...

Autoantibodies against the complement component C1q (anti-C1q) are among main biomarkers in lupus nephritis (LN) known to contribute renal injury. C1q, recognition subcomponent of classical pathway, forms a heterotetrameric complex with C1r and C1s, can also associate central regulator C1 Inhibitor (C1-Inh). However, frequency pathogenic relevance anti-C1r, anti-C1s anti-C1-Inh autoantibodies remain poorly studied LN. In this paper, we screened for anti-C1q, evaluated their association...

Lupus nephritis (LN) is a disease marked by autoantibodies against complement components. Autoantibodies negative regulator factor H (anti-FH) are prevalent in aHUS, associated with deletion of H-related protein 1 (FHR1) gene, and have overt functional consequences. They also observed C3 glomerulopathies. The frequency relevance anti-FH LN poorly studied. aim our investigation was to screen for the presence FHR1 gene cohort patients evaluate their association activity. ELISA test Western...

Vascular endothelial growth factor (VEGF) is secreted from cells and pericytes in response to hypoxia. It induces angiogenesis microvascular hyperpermeability. We observe serum VEGF concentrations some patients with vasculitic neuropathy.Plasma was measured using GenWay's human ELISA Kit, which based on standard sandwich enzyme-linked immune-sorbent assay technology. Human specific-specific monoclonal antibodies are precoated onto 96-well plates. The specific detection polyclonal...

IntroductionThe aim of the present study was to evaluate influence clinicopathological factors including age, gender, tumor grade, stage, lymphovascular invasion (LVI), necrosis and previous history non-muscle invasive bladder cancer on outcomes patients with upper tract urothelial carcinoma (UTUC) treated radical nephroureterectomy (RNU).Material methods A total 60 who underwent for at our institute between 2005 2012 were included in study.Univariate multivariate analysis performed using...

Summary Properdin is the only positive regulator of complement system. In this study, we characterize prevalence, functional consequences and disease associations autoantibodies against properdin in a cohort patients with autoimmune systemic lupus erythematosus (SLE) suffering from nephritis (LN). We detected plasma 22·5% LN (16 71) by enzyme-linked immunosorbent assay (ELISA). The binding these to was dose-dependent validated surface plasmon resonance. Higher levels anti-properdin were...

Summary Hepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. quantification in human blood may provide further insights for the pathogenesis of disorders homeostasis and might prove valuable tool clinicians differential diagnosis anaemia. This study describes ELISA immunoassay hepcidin serum. We used sandwich method from USCN Life Science inc., that consists ready to use, pre-coated 96-well strip plate with 2 antihepcidin-25 monoclonal antibodies. A recombinant 16 μg/l...

Epigenetic modifications of genomes are particular interest as numerous studies indicate the correlation between DNA methylation and development systemic lupus. As a major methyl group donor, folic acid is an important participant in this process. The aim study to determine effect low or high dose folate co-culturing with peripheral blood mononuclear cells (PBMCs) on secretion interleukin (IL)10 from regulatory lupus patients healthy volunteers.PBMCs volunteers were isolated separated CD19+...

Introduction. The syndrome of mobile claw toes or, as it is commonly called in the English-language scientific literature, checkrein deformity a rare dynamic that develops secondary to any traumatic factor at level ankle joint and foot. A special aspect clinical example presented pathology developed without direct impact on above-mentioned anatomical areas. We have not found references or descriptions such observation literature available. aim publication was demonstrate patient with...

Diabetes mellitus (DM) is a metabolic disease of chronic insulin deficiency or resistance. The progression DM associated with long-term damage to macro- and micro-vascular body systems causes serious health complications. Up 40% patients develop kidney (CKD), mainly as consequence diabetic nephropathy (DN). Hence, strict glycemic control recommended slow down CKD progression. Hyperglycemia complications in through the glycation various proteins. interaction glucose free NH2 groups proteins...

We analyzed the structural features of C1q that underlie its autoantigenicity by means a model system using an amphiphilic polyzwitterion (PZ).