A5042304122- Complement system in diseases
- Systemic Lupus Erythematosus Research
- MicroRNA in disease regulation
- Renal Diseases and Glomerulopathies
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Diet, Metabolism, and Disease
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Inflammatory Biomarkers in Disease Prognosis
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Alcohol Consumption and Health Effects
- Immunodeficiency and Autoimmune Disorders
- Cell death mechanisms and regulation
- Genomics, phytochemicals, and oxidative stress
- Colorectal and Anal Carcinomas
- Cancer Immunotherapy and Biomarkers
- Nutrition and Health in Aging
- Nuclear Structure and Function
- Genetic factors in colorectal cancer
Medical University of Varna
2016-2025
University Hospital St. Marina
2021-2022
Queen Giovanna Hospital
2020
Background: Polycystic ovary syndrome (PCOS) is the most common hyperandrogenic disorder in reproductive age women. Identifying new biomarkers with high specificity and sensitivity crucial for diagnosing monitoring disease. MiRNAs correlate PCOS-related comorbidities, suggesting their potential as biomarkers. This study examined plasma concentrations of miR-451a, miR-15a-5p, miR-320-5p, miR-28-5p, miR-103a-5p, miR-194-5p adolescents PCOS, healthy controls, controls without PCOS. Correlations...
C1q plays a key role in apoptotic cell and immune complex removal. Its absence contributes to the loss of tolerance toward self structures development autoimmunity. deficiencies are extremely rare associated with complete lack or secretion surrogate fragments. To our knowledge, we report first case functional abnormality, presence normal molecule. Homozygous GlyB63Ser mutation was found patient suffering from lupus neurologic manifestations multiple infections. The bound Igs, pentraxins,...
C1q is the recognition subunit of first component classical complement pathway. It participates in clearance immune complexes and apoptotic cells as well defense against pathogens. Inappropriate activation contributes to cellular tissue damage different pathologies, urging need for development therapeutic agents that are able inhibit system. In this study, we report heme an inhibitor C1q. Exposure significantly reduced pathway, mediated by C-reactive protein (CRP) IgG. Interaction analyses...
Colorectal cancer is one of the primary causes cancer-related deaths and 5-fluorouracil (5-FU) therapy remains cornerstone treatment in these patients. Resistance to 5-FU represents a major obstacle; therefore, finding new predictive prognostic markers crucial for improvement patient outcomes. Recently type programmed cell death was discovered-necroptosis, which depends on receptor interacting protein 3 (RIPK3). Preclinical data showed that necroptotic an important effector mechanism...
The aim of this multi-center retrospective study was to evaluate the incidence hyperprogressive disease (HPD) after second-line treatment with pembrolizumab in patients (n = 167) metastatic non-small-cell lung cancer (NSCLC) whose tumors expressed programmed cell death ligand 1 (PD-L1) ≥ 1% and search for hematological imaging biomarkers associated its development. Prior chemotherapy, neutrophil : lymphocyte ratio (NLR1) platelet (PLR1), prior immunotherapy, NLR2 PLR2 were retrospectively...
Introduction Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that can affect substantially any organ in the body. One of its most severe manifestations nephritis. Hereditary C1q deficiency strongly related to SLE but there are very few and inconsistent studies exploring single nucleotide polymorphisms (SNPs) gene cluster relation pathogenesis SLE. In present study we evaluated possible association variants complement with susceptibility nephritis Bulgarian population,...
IntroductionComplement system overactivation is pivotal in LN pathophysiology. As anti-C3 autoantibodies play a significant role pathophysiology, we explored them as disease activity biomarkers and compared to the ones against homologous protein C4.MethodsWe investigated presence of anti-C4 IgG cohort (n=85 patients) monitored their changes over time. We correlated autoantibody with clinical parameters. conducted cross-sectional longitudinal analyses (n=295 samples, 8 years follow-up)...
Autoantibodies against the complement component C1q (anti-C1q) are among main biomarkers in lupus nephritis (LN) known to contribute renal injury. C1q, recognition subcomponent of classical pathway, forms a heterotetrameric complex with C1r and C1s, can also associate central regulator C1 Inhibitor (C1-Inh). However, frequency pathogenic relevance anti-C1r, anti-C1s anti-C1-Inh autoantibodies remain poorly studied LN. In this paper, we screened for anti-C1q, evaluated their association...
Introduction International studies show an increased incidence of chronic kidney disease (CKD) in patients with metabolic syndrome (MS). It is assumed that the major components MS - obesity, insulin resistance, dyslipidemia, and hypertension are linked to renal damage through systemic release several pro-inflammatory mediators, such as uric acid (UA), C-reactive protein (CRP), generalized oxidative stress. The aim present study was investigate extent impairment manifestations dysfunction...
The pathogenesis of nonalcoholic fatty liver disease as a component metabolic syndrome (MetS) involves the activation apoptosis in steatotic hepatocytes. Caspase-generated fragments such cytokeratin-18 (CK-18) patients with various hepatic impairments are investigated markers for diagnosis and assessment severity. goal study was to capture early biomarkers elucidate their role assessing presence extent damage MetS model.We used male Wistar rats, divided into two groups (n = 7): control...
Circular RNAs (circRNAs) are a group of special endogenous long non-coding which highly stable in the circulation, and, thus, more suitable as new biomarkers colorectal cancer (CRC). The aim our study was to explore plasma expression levels four circRNAs: has_circ_0001445, hsa_circ_0003028, hsa_circ_0007915 and hsa_circ_0008717 patients with CRC evaluate their associations clinicopathological characteristics clinical outcome patients. CircRNAs were extracted from patients’ obtained prior...
RAS somatic variants are predictors of resistance to anti-EGFR therapy for colorectal cancer (CRC) and affect the outcome disease. Our study aimed evaluate frequency RAS, with a focus on KRAS variants, their association tumor location some clinicopathological characteristics in Bulgarian CRC patients. We prospectively investigated 236 patients advanced metastatic CRC. Genomic DNA was extracted from FFPE tissue samples, commercially available kits were used detect gene mutations via real-time...
Introduction. It has been shown that S-adenosylmethionine (S-AMe) stimulates glutathione synthesis and increases cell resistance to the cytotoxic action of free radicals pro-inflammatory cytokines. The aim this study was determine effect on oxidative stress in adipose tissue a model fructose-induced obesity. Methods. performed male Wistar rats divided into 3 groups: control, fructose fed (HFD) (35%, 16 weeks), HFD + S-AMe (20 mg/kg). We examined changes ratio retroperitoneal weight / body...
Lupus nephritis (LN) is a disease marked by autoantibodies against complement components. Autoantibodies negative regulator factor H (anti-FH) are prevalent in aHUS, associated with deletion of H-related protein 1 (FHR1) gene, and have overt functional consequences. They also observed C3 glomerulopathies. The frequency relevance anti-FH LN poorly studied. aim our investigation was to screen for the presence FHR1 gene cohort patients evaluate their association activity. ELISA test Western...
The present study evaluated the prognostic role of circulating miRNA-618 in patients with metastatic colon cancer (mCC) and whether miR-618 gene rs2682818 single nucleotide polymorphisms (SNP) are associated susceptibility expression levels mature miR-618. In total, 104 mCC before starting chemotherapy were investigated. status was by quantitative PCR. TaqMan PCR assay used for SNP genotyping. overexpressed serum patients. Patients high intermediate had a significantly longer mean overall...
Sulphurous mineral waters (SMW) have a wide range of applications. Sulphur content is considered as possible determinant for their anti-inflammatory or pro-inflammatory effects.To explore the healing properties Varna basin water by analysing antioxidative and effects.An intervention with SMW intake was performed healthy volunteers. Total thiols, total glutathione its fractions, reactive oxygen metabolites, malondialdehyde, intracellular adhesion molecule (ICAM-1) vascular cell (VCAM-1) were...