A5035888215- Milk Quality and Mastitis in Dairy Cows
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Liver physiology and pathology
- Probiotics and Fermented Foods
- Liver Disease Diagnosis and Treatment
- Microbial infections and disease research
- Antimicrobial Peptides and Activities
- Virology and Viral Diseases
- Microbial Metabolism and Applications
- Rabies epidemiology and control
- Reproductive Physiology in Livestock
- Animal health and immunology
- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Agriculture and Farm Safety
- Viral gastroenteritis research and epidemiology
- Streptococcal Infections and Treatments
- Pancreatitis Pathology and Treatment
- Genetic and phenotypic traits in livestock
- Cancer Mechanisms and Therapy
- Animal Genetics and Reproduction
- Vector-Borne Animal Diseases
- Biochemical and Structural Characterization
- Organ Transplantation Techniques and Outcomes
Murdoch University
2021-2025
Curtin University
2014-2024
Innovation Research Center
2017-2021
Central Agricultural University
2016-2017
Faculty of Public Health
2015
•Fn14 is upregulated on tumour cells and CAFs, TWEAK expressed by TAMs in human CCA.•TWEAK/Fn14 are progressively during rodent development.•TWEAK/Fn14 signalling induces TAM accumulation via TWEAK-inducible MCP-1 chemotaxis which can be blocked vivo.•TWEAK-inducible factors from pattern macrophages to a TAM-like phenotype.•TWEAK overexpression experimental formation drives CAF proliferation, collagen deposition increases mice. Background & AimsCholangiocarcinoma (CCA) cancer of the hepatic...
Background Biofilm formation by Staphylococcus aureus is an important virulence attribute because of its potential to induce persistent antibiotic resistance, retard phagocytosis and either attenuate or promote inflammation, depending upon the disease syndrome, in vivo. This study was undertaken evaluate significance strength biofilm clinical bovine mastitis-associated S. mammary tissue damage using a mouse mastitis model. Methods Two strains same capsular phenotype with different forming...
Staphylococcus aureus in biofilms is highly resistant to the treatment with antibiotics, which planktonic cells are susceptible. This likely be due biofilm creating a protective barrier that prevents antibiotics from accessing live pathogens buried biofilm. S. consist of an extracellular matrix comprising, but not limited to, bacterial DNA (eDNA) and poly-β-1, 6-N-acetyl-d-glucosamine (PNAG). Our study revealed despite inferiority dispersin B (an enzyme degrades PNAG) DNase I cleaves eDNA,...
The presence of unvaccinated free-roaming dogs (FRD) amidst human settlements is a major contributor to the high incidence rabies in countries where disease endemic, such as India. Estimating FRD population size crucial planning and evaluation interventions, mass immunisation, against rabies. Enumeration techniques for are resource intensive can vary from simple direct counts statistically complex capture-recapture primarily developed ecological studies. In this study we compared eight...
Abstract Rabies is an acute encephalitis caused by a lyssavirus . It primarily transmitted through bites of infected dogs which results in the worldwide death estimated 59000 humans every year. The disease preventable application post-exposure prophylaxis (PEP) and its elimination has been demonstrated many countries applying multiple interventions simultaneously. Nonetheless, rabies still widespread developing countries, due to poor implementation intervention strategies that include...
This study was undertaken to compare the immunogenicity and protective potential of biofilm vs planktonic Staphylococcus aureus vaccine for prevention mastitis using mouse as a model system. Mice immunized with formalin-killed whole cell S. residing in when delivered via an intramammary route produced mediated immune response. this showed significant reductions colonization by mammary glands, severity clinical symptoms tissue damage glands comparison mice cells aureus. The administered...
An extensive array of virulence factors associated with S. aureus has contributed significantly to its success as a major nosocomial pathogen in hospitals and community causing variety infections affected patients. Virulence include immune evading capsular polysaccharides, poly-N-acetyl glucosamine, teichoic acid addition damaging toxins including hemolytic toxins, enterotoxins, cytotoxins, exfoliative toxin, microbial surface components recognizing adhesive matrix molecules (MSCRAMM). In...
Protein A, encoded by the spa gene, is one of major immune evading MSCRAMM S. aureus, demonstrated to be prevalent in a significant percentage clinical bovine mastitis isolates Australia. Given its' reported significance biofilm formation and superior performance aureus versus planktonic vaccine mouse model, it was interest determine immunogenicity protective potential A as candidate against using model. Pregnant Balb/c mice were immunised with emulsified an alum-based adjuvant subcutaneous...
Chronic liver diseases, such as viral hepatitis, alcoholic disease, or non-alcoholic fatty are characterized by continual inflammation, progressive destruction and regeneration of the hepatic parenchyma, progenitor cell proliferation, fibrosis. The end-stage every chronic disease is cirrhosis, a major risk factor for development hepatocellular carcinoma. To study processes regulating initiation, establishment, progression, several animal models used in laboratories. Here we describe six-week...
To determine the prevalence of different capsular polysaccharide (CP) and major surface-associated non-CP antigen 336 (SP-336) types among Staphylococcus aureus isolated from bovine mastitis cases in Australia India.A total 414 strains (154 Australia, 260 India) clinical were included study. Mouse antisera raised against CP (CP1, CP2, CP5, CP8) or SP-336 used slide agglutination tests compared with detection cap1, cap5 cap8 gene fragments by PCR.Serological studies revealed presence CP8...
A cost-effective estimation of the number free-roaming dogs is an essential prerequisite for control rabies in countries where disease endemic, as vaccination at least 70% population recommended to effectively disease. Although estimating size through sight-resight based maximum likelihood methodology generates estimate closest actual size, it requires five survey efforts achieve this. In a rural setting India, reliable >70% was obtained with Application SuperDuplicates shinyapp online tool...
Mycoplasma bovis (M. bovis) can cause a multitude of diseases in cattle, with detrimental effects on the farm economy and welfare both adult young cattle. The objective this study was to determine prevalence M. cows calves south-west region Western Australia. A cross-sectional conducted 29 dairy farms 699 apparently healthy lactating 495 during 2019-2020. Nasal swabs blood samples collected from animals bulk tank milk (BTM) were assessed for bovis-specific proteins antibodies by using...
Chronic liver diseases (CLDs) are increasing in prevalence and their end-stage complications, namely, cirrhosis, failure hepatocellular carcinoma represent major global challenges. The most common initiators of progressive CLD viral hepatitis long-term alcohol abuse as well steatosis steatohepatitis. Irrespective the underlying aetiology, a feature is formation hepatic ductular reactions, involving proliferation progenitor cells (LPCs) signalling to fibrosis-driving stellate cells....
The aim of this study was to compare the performance serological versus molecular typing methods detect capsular polysaccharide (CP) and surface-associated antigen 336 phenotypes Staphylococcus aureus isolates. Molecular CP types 1, 5 8 carried out using PCR, whereas CP1, 2, 5, by slide agglutination specific antisera. By genotyping, 14/31 strains were CP8 positive, 12/31 CP5 remaining 6/31 isolates non-typable (NT). One isolate positive for both but confirmed as type serologically....
To gather data on the calf management and rearing practices of a subset dairy farmers in south-west region Western Australia.A 30-minute face-to-face survey was conducted with cattle producers Australia from April-June 2019 to determine pre-weaning practices. Participation voluntary, using self-selected registered regional extension group. The questionnaire assessed three broad categories: farm demographics, colostrum harvesting practices.The study response rate 34/140 (24%). following key...
Chronic pancreatitis increases the risk of developing pancreatic cancer through upregulation pathways favouring proliferation, fibrosis, and sustained inflammation. We established in previous studies that ligand tumour necrosis factor (TNF)-like weak inducer apoptosis (TWEAK) signals its cognate receptor fibroblast growth factor-inducible 14 (Fn14) to regulate these underlying cellular processes chronic liver injury niche. However, role TWEAK/Fn14 signalling pathway disease is entirely...
Non-typeable (NT) Staphylococcus aureus strains are associated with chronic bovine mastitis. This study investigates the impact of biofilm formation by clinical NT S. on cytokine production and mammary tissue damage using a mouse mastitis model. Mice infected two different strong weak forming potential demonstrated identical symptoms (moderate), minimal inflammatory infiltrates, (level 1 histopathological changes) in glands. However, load glands mice level pro-inflammatory cytokines (IL-1β,...
Abstract During chronic liver disease, hepatocytes may undergo proliferative arrest, leading to the activation, expansion and differentiation of hepatic progenitor cells (HPCs). Here we observe that expression A Disintegrin And Metalloprotease (ADAM) 10 is increased in human murine disease correlating with HPC expansion. We report proteolytic processing ADAM10 by ADAM9 generation an intracellular domain translocates nucleus, rather than enzymatic activity essential for regulation gene...