A5040913766- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Natural product bioactivities and synthesis
- DNA Repair Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Diabetes and associated disorders
- Immunotherapy and Immune Responses
- GABA and Rice Research
- Phytochemistry and Biological Activities
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Spectroscopy and Chemometric Analyses
- Retinal Development and Disorders
- Diet, Metabolism, and Disease
- Immune Response and Inflammation
- Nitrogen and Sulfur Effects on Brassica
- Biological Activity of Diterpenoids and Biflavonoids
- RNA and protein synthesis mechanisms
- Mast cells and histamine
- Advanced biosensing and bioanalysis techniques
Karolinska Institutet
2020-2024
Karolinska University Hospital
2020-2023
Nankai University
2016-2019
State Key Laboratory of Medicinal Chemical Biology
2017-2019
Tianjin Institute of Pharmaceutical Research (China)
2018
Ursolic acid (UA) is a pentacyclicterpenoid carboxylic that present in wide variety of plant foods. There are many beneficial health effects attributed to the properties UA. However, specific cellular targets UA and mechanism underlying downstream signal transduction processes linked anti-inflammation pathway have not been thoroughly elucidated date.
Neutrophils are the most abundant immune cells found in actively inflamed joints of patients with rheumatoid arthritis (RA), and animal models for RA depend on neutrophils induction joint inflammation. Exogenous IL-4 IL-13 protect mice from antibody-mediated inflammation, although mechanism is not understood. display a very strong basal expression STAT6, which responsible signaling following exposure to IL-13. Still, role neutrophil biology has been well studied. This can be explained by low...
Inactivating p53 mutations are the most abundant genetic alterations found in cancer. Here we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient and genes of a core CRISPR-p53 tumor suppressor interactome. Such enrichment could predispose to cancer development thus pose challenge clinical CRISPR use. Transient inhibition suppress with these mutations. The level damage response induced by an sgRNA influenced p53-deficient be relevant parameter design limit...
Dysregulated T-cell activation is a hallmark of several autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS). The lymphocyte cytosolic protein 2 (LCP2), also known SLP-76, essential for the development T cells. Despite critical role LCP2 in need developing drugs that modify activation, no inhibitors have been developed. This can be explained by "undruggable" nature LCP2, lacking structure permissive to standard small molecule inhibitor modalities. Here, we...
First report on metabolism study of ursolic acid (UA) <italic>in vivo</italic> mice.
Recent advances in single-cell sequencing technologies enable the generation of large-scale data sets paired TCR sequences from patients with autoimmune disease. Methods to validate and characterize patient-derived are needed, as well relevant model systems that can support development antigen-specific tolerance inducing drugs. We have generated a pipeline allow streamlined 'artificial' T cells robust reasonably high throughput manner for vitro vivo studies immune responses. Hereby chimeric...
Near-infrared spectroscopy (NIRS) with its fast and nondestructive advantages can be qualified for the real-time quantitative analysis. This paper demonstrates that NIRS combined partial least squares (PLS) regression used as a rapid analytical method to simultaneously quantify l-glutamic acid (l-Glu) γ-aminobutyric (GABA) in biotransformation process guide optimization of production conditions when merits are response surface methodology. The high performance liquid chromatography (HPLC)...
CRISPR/Cas9 can be used as an experimental tool to inactivate genes in cells. However, a CRISPR-targeted cell population will not show uniform genotype of the targeted gene. Instead, mix genotypes is generated - from wild type different forms insertions and deletions. Such mixed complicate analysis role gene studied population. Here, we present rapid universal approach functionally analyze that does involve generating clonal lines. As simple readout, leverage CRISPR-induced genetic...
Abstract Rheumatoid arthritis (RA) is an autoimmune disease characterized by joint inflammation, strongly associated with the activity of autoreactive CD4+ T cells. DNMT3A mutations are most common somatic found in hematopoietic system patients rheumatoid arthritis. However, role cells and follicular helper (Tfh) poorly understood. Since not identified standard genome-wide association studies, mutations’ impact on etiology diseases could be underestimated. Here, we thoroughly used KRN+...
Rheumatoid arthritis (RA) is the most common inflammatory joint disease, and early diagnosis key for effective disease management. CD69 one of earliest cell surface markers seen at activated immune cells, upregulated in synovial tissue patients with active RA. In this study, we evaluated performance a CD69-targeting PET agent, [<sup>68</sup>Ga]Ga-DOTA-Z<sub>CAM241</sub>, detection model arthritis. <b>Methods:</b> A was induced by transferring splenocytes from KRN T-cell receptor transgenic...
<div>Abstract<p>Inactivating <i>p53</i> mutations are the most abundant genetic alterations found in cancer. Here we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient and genes of a core CRISPR–p53 tumor suppressor interactome. Such enrichment could predispose to cancer development thus pose challenge clinical CRISPR use. Transient p53 inhibition suppress with these mutations. The level damage response induced by an sgRNA influenced...
Supplementary Data from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
Supplementary Data from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
Supplementary Figure from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
Supplementary Data from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
Supplementary Data from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
Supplementary Figure from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
Supplementary Data from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
Supplementary Data from CRISPR/Cas9-Induced DNA Damage Enriches for Mutations in a p53-Linked Interactome: Implications CRISPR-Based Therapies
<div>Abstract<p>Inactivating <i>p53</i> mutations are the most abundant genetic alterations found in cancer. Here we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient and genes of a core CRISPR–p53 tumor suppressor interactome. Such enrichment could predispose to cancer development thus pose challenge clinical CRISPR use. Transient p53 inhibition suppress with these mutations. The level damage response induced by an sgRNA influenced...
CRISPR/Cas9 can be used to inactivate or modify genes by inducing double-stranded DNA breaks 1–3 . As a protective cellular response, result in p53-mediated cell cycle arrest and activation of death programs 4,5 Inactivating p53 mutations are the most commonly found genetic alterations cancer, highlighting important role gene 6–8 Here, we show that cells deficient p53, as well core CRISPR-p53 tumor suppressor interactome, enriched population when CRISPR is applied. Such enrichment could pose...