A5009700567- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Stress Responses and Cortisol
- Tryptophan and brain disorders
- Receptor Mechanisms and Signaling
- Biochemical effects in animals
- Neurological Disease Mechanisms and Treatments
- Amyotrophic Lateral Sclerosis Research
- Antimicrobial Peptides and Activities
- Single-cell and spatial transcriptomics
- Autophagy in Disease and Therapy
- Photoreceptor and optogenetics research
- Machine Learning in Bioinformatics
- Nicotinic Acetylcholine Receptors Study
- Metabolomics and Mass Spectrometry Studies
- Supramolecular Self-Assembly in Materials
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Parkinson's Disease Mechanisms and Treatments
- Ion channel regulation and function
- Protein Tyrosine Phosphatases
Eötvös Loránd University
2020-2024
Amen Clinics
2021
Peripheral immune challenge induces neurobiological alterations in the brain and related neuropsychiatric symptoms both humans other mammals. One of best known physiological effects systemic inflammation is sickness behavior. However, addition to this depression-like state, there are cognitive outcomes peripherally induced neuroinflammation that can be linked dysfunction higher-order cortical areas, such as prefrontal cortex (PFC). As activity PFC largely based on balanced interplay...
Neuroinflammation induced by peripheral infections leads to various neuropsychiatric symptoms both in humans and laboratory animals, e.g., the manifestation of sickness behavior that resembles some features clinical depression. However, addition depression-like behavior, there are other acute systemic inflammation can be associated with impairment prefrontal cortex (PFC)-regulated cognitive functions. Thus, we investigated electrophysiological proteomic alterations PFC using brain slices...
In synapses that show signs of local apoptosis and mitochondrial stress undergo neuro-immunological synapse pruning, an increase in the levels presynaptic protein, neuronal-specific septin-3 can be observed. Septin-3 is a member septin GTPase family with ability to form multimers contribute cytoskeleton. However, function remains elusive. Here, we provide evidence capable binding most-studied autophagy protein Atg8 homolog microtubule-associated 1 light chain 3B (LC3B), besides another...
During chronic cerebral hypoperfusion (CCH), the blood flow gradually decreases, leading to cognitive impairments and neurodegenerative disorders, such as vascular dementia. The reduced oxygenation, energy supply induced metabolic changes, insufficient neuroplasticity could be reflected in synaptic proteome. We performed stepwise bilateral common carotid occlusions on rats studied proteome changes of hippocampus, occipital frontal cortices. Samples were prepared separated by 2-D DIGE...
Cognitive impairment and learning ability of the brain are directly linked to synaptic plasticity as measured in changes long-term potentiation (LTP) depression (LTD) animal models diseases. LTD reflects a sustained reduction AMPA receptor content based on targeted clathrin-mediated endocytosis. endocytosis is initiated by dephosphorylation Tyr876 C-terminus AMPAR subunit GluA2. The brain-specific striatal-enriched protein tyrosine phosphatase (STEP) responsible for this process. To identify...
Declining cerebral blood flow leads to chronic hypoperfusion which can induce neurodegenerative disorders, such as vascular dementia. The reduced energy supply of the brain impairs mitochondrial functions that could trigger further damaging cellular processes. We carried out stepwise bilateral common carotid occlusions on rats and investigated long-term mitochondrial, mitochondria-associated membrane (MAM), cerebrospinal fluid (CSF) proteome changes. Samples were studied by gel-based mass...
Abstract The prefrontal cortex (PFC) plays a key role in higher order cognitive functions and psychiatric disorders such as autism, schizophrenia, depression. In the PFC, two major classes of neurons are glutamatergic pyramidal (Pyr) cells GABAergic interneurons fast-spiking (FS) cells. Despite extensive electrophysiological, morphological, pharmacological studies therapeutically utilized drug targets restricted to dopaminergic, glutamatergic, receptors. To expand possibilities well better...
Abstract Background The brain extracellular fluid (ECF), composed of secreted neurotransmitters, metabolites, peptides, and proteins, may reflect processes. Analysis ECF provide new potential markers for synaptic activity or damage reveal additional information on pathological alterations. Epileptic seizure induction is an acute harsh intervention in functions, it can activate extra- intracellular proteases, which implies altered secretome. Thus, we applied a 4-aminopyridine (4-AP) epilepsy...
ABSTRACT Single-cell sequencing revealed the transcriptional heterogeneity of neurons and introduced transcriptome-defined neuron types subtypes. Temporal recordings gene transcription showed that mRNA synthesis is arranged into bursts which can be characterized by kinetic parameters. Here, we selected two distinct, functionally homogenous, electrophysiologically well-defined neuronal cell – pyramidal cells fast-spiking interneurons for burst analysis. Hierarchical clustering based on...
The investigation of the molecular background direct communication neurons and immune cells in brain is an important issue for understanding physiological pathological processes nervous system. Direct contacts between brain-infiltrating neurons, neuromodulatory effect cell-derived regulatory peptides are well established. Several aspects role glial neuro-immune also known; however, there remain many questions regarding details signaling from to cells. Thus, we report here on neuronal...