A5078001022- Peanut Plant Research Studies
- Coconut Research and Applications
- Plant Virus Research Studies
- Agricultural pest management studies
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Nitrogen and Sulfur Effects on Brassica
- Enterobacteriaceae and Cronobacter Research
- Light effects on plants
- Soybean genetics and cultivation
- Mycotoxins in Agriculture and Food
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Genetic and Environmental Crop Studies
- Photosynthetic Processes and Mechanisms
- Plant pathogens and resistance mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Management Techniques
- RNA and protein synthesis mechanisms
- Seed Germination and Physiology
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Sirtuins and Resveratrol in Medicine
- Seed and Plant Biochemistry
Sichuan Academy of Agricultural Sciences
2025
Guangdong Academy of Agricultural Sciences
2015-2024
South China Normal University
2010-2012
Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil protein. To explore the origins consequences of tetraploidy, we sequenced A. hypogaea genome compared it with related diploid Arachis duranensis ipaensis genomes. We annotated 39 888 A-subgenome genes 41 526 B-subgenome peanut. The subgenomes have evolved asymmetrically, B subgenome resembling ancestral state A undergoing more gene disruption, loss, conversion, transposable...
Significance We present a draft genome of the peanut A-genome progenitor, Arachis duranensis , providing details on total genes in genome. Genome analysis suggests that lineage was affected by at least three polyploidizations since origin eudicots. Resequencing synthetic tetraploids reveals extensive gene conversion their formation human hands. The A. provides major source candidate for fructification, oil biosynthesis, and allergens, expanding knowledge understudied areas plant biology...
Abstract Single nucleotide polymorphisms (SNPs) are the most abundant DNA sequence variation in genomes which can be used to associate genotypic phenotype. Therefore, availability of a high-density SNP array with uniform genome coverage advance genetic studies and breeding applications. Here we report development ‘Axiom _Arachis ’ 58 K SNPs its utility groundnut diversity study. In this context, from total 163,782 derived resequencing RNA-sequencing 41 accessions wild diploid ancestors,...
Lack of sufficient molecular markers hinders current genetic research in peanuts (Arachis hypogaea L.). It is necessary to develop more for potential use peanut research. With the development EST projects, a vast amount available sequence data has been generated. These offered an opportunity identify SSR ESTs by mining. In this study, we investigated 24,238 identification and markers. total, 881 SSRs were identified from 780 SSR-containing unique ESTs. On average, one was found per 7.3 kb...
Abstract Background The construction of genetic linkage maps for cultivated peanut ( Arachis hypogaea L.) has and continues to be an important research goal facilitate quantitative trait locus (QTL) analysis gene tagging use in a marker-assisted selection breeding. Even though few have been developed, they were constructed using diploid or interspecific tetraploid populations. most recently published intra-specific map was from the cross peanuts, which only 135 simple sequence repeat (SSR)...
Peanut is an important and nutritious agricultural commodity a livelihood of many small-holder farmers in the semi-arid tropics (SAT) world which are facing serious production threats. Integration genomics tools with on-going genetic improvement approaches expected to facilitate accelerated development improved cultivars. Therefore, high-resolution genotyping multiple season phenotyping data for 50 agronomic, disease quality traits were generated on ‘reference set’ peanut. This study reports...
Background MicroRNAs (miRNAs) are noncoding RNAs of approximately 21 nt that regulate gene expression in plants post-transcriptionally by endonucleolytic cleavage or translational inhibition. miRNAs play essential roles numerous developmental and physiological processes many them conserved across species. Extensive studies have been done a few model plants; however, less is known about the diversity these regulatory peanut (Arachis hypogaea L.), one most important oilseed crops cultivated...
The complex, tetraploid genome structure of peanut (Arachis hypogaea) has obstructed advances in genetics and genomics the species. aim this study is to understand Arachis by developing a high-density integrated consensus map. Three recombinant inbred line populations derived from crosses between A diploid species, duranensis stenosperma; B ipaënsis magna; AB tetraploids, A. hypogaea an artificial amphidiploid (A. × duranensis)4×, were used construct genetic linkage maps: 10 groups (LGs) 544...
Summary Single‐cell RNA‐seq (scRNA‐seq) has been highlighted as a powerful tool for the description of human cell transcriptome, but technology not broadly applied in plant cells. Herein, we describe successful development robust protoplast isolation system peanut leaf. A total 6,815 single cells were divided into eight clusters based on reported marker genes by applying scRNA‐seq. Further, pseudo‐time analysis was used to developmental trajectory and interaction network transcription...
Summary Although the regulatory mechanisms of dark and light‐induced plant morphogenesis have been broadly investigated, biological process in peanuts has not systematically explored on single‐cell resolution. Herein, 10 cell clusters were characterized using scRNA‐seq‐identified marker genes, based 13 409 11 296 single cells from 1‐week‐old peanut seedling leaves grown under light conditions. 6104 genes 50 transcription factors (TFs) displayed significant expression patterns distinct...
Only a few genetic maps based on recombinant inbred line (RIL) and backcross (BC) populations have been developed for tetraploid groundnut. The marker density, however, is not very satisfactory especially in the context of large genome size (2800 Mb/1C) 20 linkage groups (LGs). Therefore, using segregation data 10 RILs one BC population from international groundnut community, with help common markers across different populations, reference consensus map has developed. This comprised 897 loci...
Background Germin-like superfamily members are ubiquitously expressed in various plant species and play important roles development defense. Although several GLPs have been identified peanut (Arachis hypogaea L.), their defense remain unknown. In this research, we study the spatiotemporal expression of AhGLPs functions Results We three new AhGLP (AhGLP3b, AhGLP5b AhGLP7b) that distinct but very closely related DNA sequences. The spatial temporal profiles revealed each GLP gene has its...
Summary The failure of peg penetration into the soil leads to seed abortion in peanut. Knowledge genes involved these processes is comparatively deficient. Here, we used RNA ‐seq gain insights transcriptomes aerial and subterranean pods. More than 2 million transcript reads with an average length 396 bp were generated from one ( AP ) two SP 1 2) pod libraries using pyrosequencing technology. After assembly, sets 49 632, 952 50 494 a total 74 974 assembly contigs TAC s) identified , 2,...
The growth of Flammulina filiformis is strongly dependent on low-temperature cues for the initiation primordia formation. To obtain a comprehensive understanding molecular mechanisms that govern mycelial response to cold stress, de novo genome sequencing F. monokaryon and multi-omics data (transcriptome metabolome) analyses mycelia, primordia, fruiting bodies were conducted in present study. Genome based PacBio HiFi Hi-C resulted 36.3 Mb sequence mapped 12 chromosomes, comprising 11,886...
Abstract Background Pre-harvest infection of peanuts by Aspergillus flavus and subsequent aflatoxin contamination is one the food safety factors that most severely impair peanut productivity human animal health, especially in arid semi-arid tropical areas. Some cultivars with natural pre-harvest resistance to have been identified through field screening. However, little known about mechanism, which has slowed incorporation into commercially acceptable genetic background. Therefore, it...
Peanut (Arachis hypogaea L.), an important leguminous crop, is widely cultivated in tropical and subtropical regions. allotetraploid, having A B subgenomes that maybe have originated its diploid progenitors Arachis duranensis (A-genome) ipaensis (B-genome), respectively. We previously sequenced the former here present draft genome of latter, expanding our knowledge unique biology Arachis. The assembled A. ~1.39 Gb with 39,704 predicted protein-encoding genes. gene family analysis revealed...
Legumes are important food crops worldwide, contributing to more than 33% of human dietary protein. The production crop legumes is frequently impacted by abiotic and biotic stresses. It therefore identify genes conferring resistance stresses tolerance that can be used both understand molecular mechanisms plant response the environment accelerate improvement. Recent advances in genomics offer a range approaches such as sequencing genomes transcriptomes, gene expression microarray well RNA-seq...
A characteristic feature of peanut is the subterranean fructification, geocarpy, in which gynophore ('peg'), a specialized organ that transitions from upward growth habit to downward outgrowth upon fertilization, drives developing pod into soil for subsequent development underground. As step towards understanding this phenomenon, we explore developmental dynamics transcriptome at 11 successive stages. We identified 110 217 transcripts across stages and quantified their abundance along...
Many large-effect quantitative trait loci (QTLs) for yield and disease resistance related traits have been identified in different mapping populations of peanut (Arachis hypogaea L.) under multiple environments. However, only a limited number QTLs used marker-assisted selection (MAS) because unfavorable epistatic interactions between genetic backgrounds. Thus, it is essential to identify consensus across environments backgrounds use MAS. Here, we QTL meta-analysis set peanut.A new integrated...