The lack of suitable experimental animal models for persistent pain showing clear acupuncture analgesia, has been the major stumbling block in investigation physiological mechanisms analgesia. present study developed a new rat model ankle sprain and effect electroacupuncture (EA) on this was examined. A common source humans is lateral sprain. To condition, rat's right bent repeatedly, overextending ligaments, 4 min under halothane anesthesia. subsequently showed swelling reduced stepping...

In a previous study, we showed that electroacupuncture (EA) applied to the SI-6 point on contralateral forelimb produces long-lasting and powerful analgesia in pain caused by ankle sprain rat model. To investigate underlying mechanism of EA analgesia, present study tested effects various antagonists known endogenous analgesic systems this Ankle was induced anesthetized rats overextending their right with repeated forceful plantar flexion inversion foot. When developed behaviors (a reduction...

It has been reported that acupuncture at GB34 can enhance neurogenesis in the subventricular zone (SVZ) of mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). However, signaling pathway plays a critical role needs to be established. Herein, we investigated neurogenesis-promoting mediated by acupuncture, focusing on extracellular signal-regulated kinase (ERK) signaling.Male 10-week-old C57BL/6 were intraperitoneally injected 30 mg/kg MPTP once daily for 5 days....

Sophora flavescens Aiton (SF) has been used to treat various diseases including fever and inflammation in China, Korea Japan. Several recent reports have shown that SF anti-inflammatory anti-apoptotic effects, indicating it is a promising candidate for treatment of Parkinson's disease (PD). We evaluated the protective effect against neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+) -induced mitochondrial dysfunction SH-SY5Y human neuroblastoma cells, an vitro PD model. cells were incubated...

This study was performed to determine whether spinal cholinergic systems mediate the relieving effects of electroacupuncture (EA) on cold and warm allodynia in a rat model neuropathic pain. For surgery, right superior caudal trunk resected at level between S1 S2 nerves innervating tail. Two weeks after injury, intrathecal (i.t.) catheter implanted. Five days catheterization, rats were injected with atropine (non-selective muscarinic antagonist, 30 μg), mecamylamine nicotinic 50 pirenzepine...

Ginseng is known to have antiapoptotic, anti-inflammatory, and antioxidant effects. The present study investigated a possible role of Korean Red (KRG) in suppressing dopaminergic neuronal cell death the cleavage p35 p25 substantia nigra (SN) striatum (ST) using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease mouse model.Ten-week-old male C57BL/6 mice were injected intraperitoneally with 30 mg/kg MPTP at 24-h intervals for 5 d, then administered KRG (1 mg/kg,...

Recent studies have shown that Korean Red Ginseng (KRG) suppresses dopaminergic neuronal death in the brain of a Parkinson's disease (PD) mouse model, but mechanism is still elusive. Using 2-dimensional electrophoresis technique, we investigated whether KRG can restore changes protein expressions striatum (ST) 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-injected mice. Male C57BL/6 mice (9 weeks old) were injected with 20 mg/kg MPTP intraperitoneally four times at 2-h intervals. (100...

Modulation of endogenous neurogenesis would have a significant impact on future therapeutic strategies for neurodegenerative diseases. Recent studies suggested that the enhancement adult can be helpful in treatment Parkinson's disease (PD). In this study, we investigated augmentative effects Korean Red Ginseng (KRG) subventricular zone (SVZ) PD mouse model. Male eight-week-old C57BL/6 mice were injected with vehicle or 20 mg/kg 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) four times...

This study aimed to compare the effects of high frequency electroacupuncture (EA) and low-frequency EA on autonomic nervous system by using a heart rate variability measuring device in normal individuals. Fourteen participants were recruited each participated high-frequency sessions (crossover design). The order was randomized interval between two over 2 weeks. Participants received needle insertion with 120-Hz stimulation during session (high-frequency group), 2-Hz (low-frequency group)....

목적 : 본 연구는 퇴행성 슬관절염 환자의 통증을 줄이고 증상을 개선하는데 있어 현재 침구임상에서 활용되고 있는 온침이 침보다 더욱 유효한지를 알아보기 위하여 임상연구로 시행되었다. 방법 총 76명의 지원자가 연구에 참가하였으며, 이들 모두는 선정기준과 제외기준에 의하여 선발되었고 최종적으로 임상연구를 마친 지원자는 66명이었다. 지원자들은 무작위로 온침군과 침군 중에 할당이 되었다. 실험군(온침)에 속한 38명은 온침시술을, 대조군(침)에 침시술을 받았으며, 8주 동안 16회의 시술을 환측의 무릎에 시행하였다. 주된 평가척도는 Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)이었고, 부수적 physical health score based on the 36-Item Short-Form Health Survey(SF-36), Patient Global Assessment이었다. 결과 실험군이 대조군에...

Recent studies have suggested that increased oxidative stress is a potential etiology in Parkinson’s disease (PD). In this study, we investigated whether acupuncture regulates antioxidants the striatum (ST) of PD mouse model. Male C57BL/6 mice were administered 30 mg/kg 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intraperitoneally once day for 5 days and given stimulation at SI3 or GB34 (Yanglingquan) was 12 consecutive days. Dopaminergic neuronal survival nigrostriatal pathway DJ-1...

Nefopam has a pharmacologic profile distinct from that of opioids or other anti-inflammatory drugs. Several recent studies demonstrate nefopam mechanism action similar to those anti-depressants and anticonvulsants for treating neuropathic pain. The present study investigates the mechanical antiallodynic effect using immunohistochemical western blot analysis in rat pain model. Twenty-eight male Sprague-Dawley rats were subjected left fifth lumbar (L5) spinal nerve ligation intrathecal...

Background: Korean Red Ginseng (KRG) is a valuable herb in Asian countries that used as crude substance to inhibit inflammation and enhance vitality, longevity immunity. The protective effects of KRG against the toxicity 1-methyl-4-phenylpyridinium (MPP+) were investigated vitro present study.Methods: PC12 cells pretreated with water extract for 24 h, then incubated MPP+ h. growth was assessed using live cell viability assay, ratio apoptotic measured flow cytometry morphology detected...

This study was performed to examine whether electroacupuncture potentiates the neostigmine-induced antiallodynia in neuropathic pain rats. Although intrathecal neostigmine (0.05, 0.1, and 0.3 μg) dose-dependently relieved cold allodynia, μg caused side effects. The coapplication of 0.1 electroacupuncture, however, produced potent antiallodynia, which parallel effect neostigmine, without These results indicate that can enhance antiallodynic action neostigmine.

This study examined whether manual acupuncture at the Siguan points (bilateral LI4 and LR3) affects intestinal motility in healthy human subjects. Twenty male subjects were randomly assigned either to real (RA) or sham (SA) groups a crossover manner. All underwent two experimental sessions; RA group first session was treated with SA second after 2-week washout period, vice versa. Each subject took 20 radio-markers 0, 12, 24, 36 hours radio-marker intake. Radiographs taken 6, 12.5, 24.5, 48...

Adult neurogenesis has recently been considered a new therapeutic paradigm of Parkinson’s disease. In this study, we investigated whether acupuncture restores 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine- (MPTP-) induced impaired in the subventricular zone (SVZ). Male C57BL/6 mice were given 30 mg/kg MPTP intraperitoneally once day for 5 days, after which they injected with 50 bromodeoxyuridine (BrdU) and stimulation at HT7 or GB34 12 consecutive days. Dopaminergic neuronal survival...

We investigated the inhibitory pathways that mediate antinociceptive effects of heterotopic electro-acupuncture (EA) on formalin injection-induced pain in rats. EA (2 ms, 10 Hz, 3 mA) was delivered to acupoints HT 7 and PC for 30 min; this followed immediately by subcutaneous injection into left hind paw Naltrexone (10 mg/kg, i.p.), an opioid receptor antagonist, administered evaluate involvement endogenous opioids. The dorsolateral funiculus (DLF), which is a descending pathway inhibits...

This study was conducted to compare the effects of low frequency electroacupuncture (EA) and high EA at acupoint ST36 on production IgE Th1/Th2 cytokines in BALB/c mice that had been immunized with 2,4-dinitrophenylated keyhole limpet protein (DNP-KLH), as well investigate difference immunomodulatory exerted by stimulations a non-acupoint (tail). Female were divided into seven groups: normal (no treatments), IM (immunization only), ST36-PA (IM + plain acupuncture ST36), ST36-LEA (1 Hz)...