A5044880309- Bacterial Genetics and Biotechnology
- Lipid Membrane Structure and Behavior
- Plant Stress Responses and Tolerance
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- Fungal and yeast genetics research
- Amino Acid Enzymes and Metabolism
- Nicotinic Acetylcholine Receptors Study
- bioluminescence and chemiluminescence research
- Mitochondrial Function and Pathology
- Molecular Sensors and Ion Detection
- Glycosylation and Glycoproteins Research
- Antibiotic Resistance in Bacteria
- Mass Spectrometry Techniques and Applications
- Muscle Physiology and Disorders
- DNA and Nucleic Acid Chemistry
- Neurobiology and Insect Physiology Research
- Protein Structure and Dynamics
- Pediatric Hepatobiliary Diseases and Treatments
- Protein purification and stability
- Tissue Engineering and Regenerative Medicine
The University of Texas Health Science Center at Houston
2011-2024
Nanjing Drum Tower Hospital
2024
Hebei Medical University
2023
Shanghai Ninth People's Hospital
2022
Shanghai Jiao Tong University
2022
Fujian Agriculture and Forestry University
2021
Zhejiang Institute of Freshwater Fisheries
2021
Jilin University
2021
Paul Scherrer Institute
2004-2006
Lomonosov Moscow State University
2005
We have developed a new primer design method based on the QuickChange TM site-directed mutagenesis protocol, which significantly improves PCR amplification efficiency.This minimizes dimerization and ensures priority of primer-template annealing over self-pairing during PCR.Several different multiple mutations (up to 7 bases) were successfully performed with this partial overlapping in variety vectors ranging from 4 12 kb length.In comparison, all attempts failed when using...
Ammonium is one of the most important nitrogen sources for bacteria, fungi, and plants, but it toxic to animals. The ammonium transport proteins (methylamine permeases/ammonium transporters/rhesus) are present in all domains life; however, functional studies with members this family have yielded controversial results respect chemical identity (NH(4)(+) or NH(3)) transported species. We solved structure wild-type AmtB from Escherichia coli two crystal forms at 1.8- 2.1-A resolution,...
The anion channelrhodopsin GtACR1 from the alga Guillardia theta is a potent neuron-inhibiting optogenetics tool. Presented here, its X-ray structure at 2.9 Å reveals tunnel traversing protein extracellular surface to large cytoplasmic cavity. lined primarily by small polar and aliphatic residues essential for conductance. A disulfide-immobilized cap facilitates channel closing ion path blocked mid-membrane photoactive retinylidene chromophore further side constriction. also novel site...
Kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) is a light-gated channel used for optogenetic silencing of mammalian neurons. It selects K
Amt proteins constitute a class of ubiquitous integral membrane that mediate movement ammonium across cell membranes. They are homotrimers, in which each subunit contains narrow pore through substrate transport occurs. Two conserved histidine residues the have been proposed to be necessary for ammonia conductance. By analyzing 14 engineered polar and non-polar variants these histidines, Escherichia coli AmtB, we show both histidines absolutely required optimum Crystal structures confirm...
Ca 2+ efflux by cation antiporter (CaCA) proteins is important for maintenance of homeostasis across the cell membrane. Recently, monomeric structure prokaryotic Na + /Ca exchanger (NCX) NCX_Mj protein from Methanococcus jannaschii shows an outward-facing conformation suggesting a hypothesis alternating substrate access efflux. To demonstrate conformational changes essential CaCA mechanism, we present crystal /H YfkE Bacillus subtilis at 3.1-Å resolution. forms homotrimer, confirmed...
Secretory phospholipases A2 (sPLA2s) are potent components of mammalian innate-immunity antibacterial mechanisms. sPLA2 enzymes attack bacteria by hydrolyzing bacterial membrane phospholipids, causing disorganization and cell lysis. However, most Gram-negative naturally resistant to Here we report a novel resistance mechanism in Escherichia coli, mediated phospholipid repair system consisting the lysophospholipid transporter LplT acyltransferase Aas cytoplasmic membrane. Mutation lplT or aas...
Lysophospholipid transporter (LplT) was previously found to be primarily involved in 2-acyl lysophosphatidylethanolamine (lyso-PE) recycling Gram-negative bacteria. This work identifies the potent role of LplT maintaining membrane stability and integrity Escherichia coli envelope. Here we demonstrate involvement three major bacterial phospholipids using a combination an vitro lysophospholipid binding assay purified protein transport assays with E. spheroplasts. Our results show that lyso-PE...
Na+/Ca2+ exchangers (NCX) constitute a major Ca2+ export system that facilitates the re-establishment of cytosolic levels in many tissues. interactions at its binding domains (CBD1 and CBD2) are essential for allosteric regulation exchange activity. The structure Ca2+-bound form CBD1, primary sensor from canine NCX1, but not Ca2+-free form, has been reported, although molecular mechanism remains unclear. Here, we report crystal structures three distinct states CBD1 CALX, exchanger found...
The regeneration of skin tissue is often impeded by bacterial infection seriously. At the same time, reactive oxygen species (ROS) are overexpressed in infected wounds, causing persistent inflammation that further hinders repair process. All these make treatment wounds still a great challenge clinic. In this study, we fabricate Cu(II)@MXene photothermal complex based on electrostatic self-assembly between Cu2+ and MXene, which then introduced into hyaluronic acid (HA) hydrogel to form an...
Journal Article Production of a Functional Catalytic Antibody ScFv-NusA Fusion Protein in Bacterial Cytoplasm Get access Lei Zheng, Zheng Search for other works by this author on: Oxford Academic PubMed Google Scholar Ulrich Baumann, Baumann Jean-Louis Reymond The Biochemistry, Volume 133, Issue 5, May 2003, Pages 577–581, https://doi.org/10.1093/jb/mvg074 Published: 01 2003
Lysophospholipid transporter (LplT) is a member of the major facilitator superfamily present in many Gram-negative bacteria. LplT catalyzes flipping lysophospholipids (LPLs) across bacterial inner membrane, playing an important role membrane homeostasis. We previously reported that promotes both uptake exogenous LPLs and intramembranous LPL bilayer. To gain mechanistic insight into this dual LPL-flipping activity, here we implemented combination computational approaches transport analyses to...
Abstract The cyclic ammonium cation 5 and its guanidinium analogue 4 are inhibitors of tocopherol cyclase. Monoclonal antibodies were raised against protein conjugates the haptens 1 – 3 screened for catalytic reactions with alkene 8 , a short chain natural substrate phytyl‐hydroquinone 6 enol ether analogues 10 b . Antibody 16E7 hapten was found to catalyze hydrolysis Z form hemiacetal 12 an apparent rate acceleration k cat / uncat =1400. also catalyzed elimination Kemp's benzisoxazole 59...
The crystal structure of the light-gated anion channel GtACR1 reported in our previous Research Article (Li et al., 2019) revealed a continuous tunnel traversing protein from extracellular to intracellular pores. We proposed as conductance closed by three constrictions: C1 half, mid-membrane C2 containing photoactive site, and C3 on cytoplasmic side. Reported here, bromide-bound reveals structural changes that relax constrictions, including novel salt-bridge switch mechanism involving site....
Identifying secretory proteins from blood, saliva or other body fluids has become an effective method of diagnosing diseases. Existing protein prediction methods are mainly based on conventional machine learning algorithms and highly dependent the feature set protein. In this article, we propose a deep model capsule network transformer architecture, SecProCT, to predict using only amino acid sequences. The proposed was validated cross-validation achieved 0.921 0.892 accuracy for predicting...
Catalytic antibody 14D9 catalyzes the enantioselective protonation of prochiral enol ethers with high enantioselectivity (>99% ee ) and a practical turnover ( k cat = 0.4 s -1 ), allowing for preparative scale applications. This represents one rare examples catalytic antibodies promoting acid-catalyzed processes. Antibody was cloned expressed as chimeric Fab fragment in Escherichia coli . Crystal structures apo form its close analog 19C9 complex transition state were determined at 2.8-Å...