A5083632870- Parkinson's Disease Mechanisms and Treatments
- Multiple Sclerosis Research Studies
- Mitochondrial Function and Pathology
- Peripheral Neuropathies and Disorders
- Adipose Tissue and Metabolism
- Nuclear Receptors and Signaling
- Autophagy in Disease and Therapy
- Acute Ischemic Stroke Management
- Intracerebral and Subarachnoid Hemorrhage Research
- Myasthenia Gravis and Thymoma
- Genetic Neurodegenerative Diseases
- Systemic Lupus Erythematosus Research
- Neurological diseases and metabolism
- Signaling Pathways in Disease
- Hereditary Neurological Disorders
- RNA regulation and disease
- Alzheimer's disease research and treatments
- Biochemical effects in animals
- Advanced Neuroimaging Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Mosquito-borne diseases and control
- Neuroinflammation and Neurodegeneration Mechanisms
- Effects and risks of endocrine disrupting chemicals
- Rheumatoid Arthritis Research and Therapies
- Infectious Encephalopathies and Encephalitis
Hong Kong Polytechnic University
2025
City University of Hong Kong
2024-2025
University of Hong Kong
2015-2024
Chinese University of Hong Kong
2007-2023
Queen Mary Hospital
2006-2022
Hospital Authority
2021
University of Oxford
2017
Queen Mary University of London
2012
York District Hospital
2010
Mayo Clinic
2007-2008
In patients with TIA and ischemic stroke, we validated the total small vessel disease (SVD) score by determining its prognostic value for recurrent stroke.Two independent prospective studies were conducted, one comprising predominantly Caucasian TIA/ischemic stroke (Oxford Vascular Study [OXVASC]) Chinese (University of Hong Kong [HKU]). Cerebral MRI was performed assessed lacunes, microbleeds, white matter hyperintensities (WMH), perivascular spaces (PVS). Predictive SVD risk determined...
Parkinson disease (PD) is an age-related neurodegenerative disorder associated with misfolded SNCA/α-synuclein accumulation in brain. Impaired catabolism of SNCA potentiates formation its toxic oligomers. LRRK2 (leucine-rich repeat kinase-2) mutations predispose to familial and sporadic PD. Mutant perturbs chaperone-mediated-autophagy (CMA) degrade SNCA. We showed greater age-dependent oligomeric striatum cortex aged LRRK2R1441G knockin (KI) mice, compared age-matched wildtype (WT) by 53%...
Autosomal dominant mutations that activate the leucine-rich repeat kinase 2 (LRRK2) cause inherited Parkinson's disease. Recent work has revealed LRRK2 directly phosphorylates a conserved threonine/serine residue in effector-binding switch-II motif of number Rab GTPase proteins, including Rab10. Here we describe facile and robust method to assess phosphorylation endogenous Rab10 mouse embryonic fibroblasts (MEFs), lung spleen-derived B-cells, based on ability Phos-tag reagent retard...
Abstract We systematically evaluated the frequency of neurological disorders and muscle neural autoantibodies in 177 patients with neuromyelitis optica (NMO) 250 control subjects (173 healthy; 77 multiple sclerosis, MS, patients). An excess myasthenia gravis (MG, 2%), muscle‐type acetylcholine receptor antibody (11%) was detected among NMO patients. The presence or more common (34%) than MS healthy controls (7%), P < 0.0001. coexistence MG should be considered atypical refractory...
Insulin resistance is the major pathogenesis underlying type 2 diabetes mellitus (T2DM) and these patients have doubled risk of Alzheimer's disease (AD). Increasing evidence suggests that insulin plays an important role in AD pathogenesis, possibly due to abnormal GSK3β activation, causing intra- extracellular amyloid-beta (Aβ) accumulation. Adiponectin (APN) adipokine with insulin-sensitizing anti-inflammatory effects. Reduced circulatory APN level associated T2DM. The has not been...
Mutations that activate the LRRK2 (leucine-rich repeat protein kinase 2) predispose to Parkinson's disease, suggesting inhibitors might have therapeutic benefit. Recent work has revealed phosphorylates a subgroup of 14 Rab proteins, including Rab10, at specific residue located centre its effector-binding switch-II motif. In present study, we analyse selectivity and sensitivity polyclonal monoclonal phospho-specific antibodies raised against nine different LRRK2-phosphorylated proteins...
Beta-amyloid (Aβ ) neurotoxicity is important in Alzheimer's disease (AD) pathogenesis. Aβ causes oxidative stress, inflammation and mitochondrial damage resulting neuronal degeneration death. Oxidative failure are also pathophysiological mechanisms of type 2 diabetes (T2DM) which characterized by insulin resistance. Interestingly, T2DM increases risk to develop AD associated with reduced sensitivity (central resistance). We studied the potential protective effect adiponectin (an adipokine...
Uncoupling proteins (UCPs) belong to a large family of mitochondrial solute carriers 25 (SLC25s) localized at the inner membrane. UCPs transport protons directly from intermembrane space matrix. Of five structural homologues (UCP1 5), UCP4 and 5 are principally expressed in central nervous system (CNS). Neurons derived their energy form ATP that is generated through oxidative phosphorylation carried out by multiprotein complexes (Complexes I-V) embedded In phosphorylation, flow electrons...
Mitochondrial dysfunction causes energy deficiency and nigrostriatal neurodegeneration which is integral to the pathogenesis of Parkinson disease (PD). Clearance defective mitochondria involves fission ubiquitin-dependent degradation via mitophagy maintain homeostasis. We hypothesize that LRRK2 (leucine-rich repeat kinase 2) mutation disrupts mitochondrial turnover causing accumulation in aging brain. found more ubiquitinated with aberrant morphology associated impaired function aged (but...
Abstract Two anti‐neuronal nuclear antibodies (ANNA‐1 and ANNA‐2) are markers of paraneoplastic neurological autoimmunity related to small‐cell carcinoma. ANNA‐2 is also breast Here we define a third IgG specificity (ANNA‐3), identified in 11 patients (10 adults) by immunofluorescence screening sera from approximately 68,000 with suspected syndromes. ANNA‐3 binds prominently nuclei cerebellar Purkinje neurons, not cytoplasm, granular or enteric but distinctively renal glomerular podocytes....
Abstract We determined the prevalence of muscle acetylcholine receptor (AChR) antibodies in patients with adult‐acquired generalized myasthenia gravis (MG), seroconversion rate at 12 months, and muscle‐specific tyrosine kinase (MuSK) antibody among persistently seronegative patients. identified 562 consecutive Mayo Clinic MG based on clinical electrophysiological criteria. At presentation, 508 (90.4%) tested positive for AChR binding or modulating antibodies. After 15.2% initially had become...
Background: Neuromyelitis optica spectrum disorders (NMOSDs) are severe inflammatory demyelinating of the central nervous system.Brain involvement is increasingly recognized.Objective: To study brain in NMOSDs among Hong Kong Chinese patients.Design: Retrospective patients with NMOSDs.Setting: Tertiary medical center Kong.Patients: Thirty-four 2 years or longer were recruited.Interventions: Brain and spinal cord magnetic resonance imaging was performed during NMOSD attacks repeated yearly...
This review gives a brief insight into the role of mitochondrial dysfunction and oxidative stress in converging pathogenic processes involved Parkinson's disease (PD). Mitochondria provide cellular energy form ATP via phosphorylation, but as an integral part this process, superoxides other reactive oxygen species are also produced. Excessive free radical production contributes to stress. Cells have evolved handle such various endogenous anti-oxidant proteins. One family proteins is...
Abstract Aging, genetics and environmental toxicity are important etiological factors in Parkinson’s disease (PD). However, its pathogenesis remains unclear. A major obstacle is the lack of an appropriate experimental model which incorporates genetic susceptibility, aging prolonged toxicity. Here, we explored interplay amongst these using mutant LRRK2 R1441G (leucine-rich-repeat-kinase-2) knockin mice. We found that primary cortical mesencephalic dopaminergic neurons were more susceptible to...
Introduction: Clinical guidelines recommend a holistic approach to Parkinson’s disease (PD) care, yet randomized trials examining mindfulness-based interventions in this context are scarce. This study investigated the effects of two mindfulness practices – meditation and yoga on biopsychosocial outcomes PD patients, including anxiety symptoms, depressive motor/nonmotor health-related quality-of-life (HRQOL), mindfulness, stress inflammation biomarkers, compared usual care. Methods: 159...
<b><i>Background/Aims:</i></b> Multiple sclerosis (MS) causes significant neurological disability. Experimental autoimmune encephalomyelitis (EAE) is an animal model of MS. Human bone marrow mesenchymal stem cells (hMSCs) possess anti-inflammatory and immunosuppressive effects. We studied whether hMSCs affect CD1d<sup>high</sup>CD5<sup>+</sup> regulatory B-cell activity in EAE. <b><i>Methods:</i></b> EAE was induced...