A5080592486- Neuroinflammation and Neurodegeneration Mechanisms
- Extracellular vesicles in disease
- Alzheimer's disease research and treatments
- Neurological Disease Mechanisms and Treatments
- Immune cells in cancer
- Dementia and Cognitive Impairment Research
- Disaster Response and Management
- Metabolomics and Mass Spectrometry Studies
- S100 Proteins and Annexins
- Mass Spectrometry Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Intracerebral and Subarachnoid Hemorrhage Research
- Chemokine receptors and signaling
- Cardiac Arrest and Resuscitation
- Heme Oxygenase-1 and Carbon Monoxide
- Functional Brain Connectivity Studies
- Psychosomatic Disorders and Their Treatments
- Simulation-Based Education in Healthcare
- Traumatic Brain Injury and Neurovascular Disturbances
- Trauma and Emergency Care Studies
- Infrared Thermography in Medicine
- Memory and Neural Mechanisms
- Emergency and Acute Care Studies
- Paranormal Experiences and Beliefs
Western University
2019-2024
New York Medical College
2019-2020
Sunnybrook Health Science Centre
2017
Sunnybrook Research Institute
2017
The brain's response to acute injury is characterized by increased permeability of the blood-brain barrier (BBB) and pro-inflammatory microglia signaling, both which have been linked poor cognitive outcomes neurological disease. damaged BBB has leakiness, allowing serum proteins like fibrinogen into brain, interacts with local cells in a deleterious manner. At same time, injury, demonstrate NLRP3 inflammasome activity heightened release cytokines. relationship between uptake microglial...
Abstract Chronic microglia activation post-stroke is associated with worse neurological and cognitive outcomes. However, measurement of in vivo currently limited. Plasma derived extracellular vesicles (EVs) are cell-specific indicators that may allow for non-invasive phenotype. The aim this study was to identify activation-state specific EVs (MEVs) vitro followed by validation an experimental stroke model. Following pro-inflammatory activation, MEVs contain the protein TMEM119 alongside...
Abstract INTRODUCTION Accurate testing for Alzheimer's disease (AD) represents a crucial step therapeutic advancement. Currently, tests are expensive and require invasive sampling or radiation exposure. METHODS We developed nanoscale flow cytometry (nFC)‐based assay of extracellular vesicles (EVs) to screen biomarkers in plasma from mild cognitive impairment (MCI), AD, controls. RESULTS Circulating amyloid beta (Aβ), tau, phosphorylated tau (p‐tau)181, p‐tau231, p‐tau217, p‐tauS235,...
Matrix assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is used to perform spectrometric analysis directly on biological samples providing visual and anatomical spatial information molecules within tissues. A current obscuration of MALDI-IMS that it largely performed fresh frozen tissue, whereas clinical tissue stored long-term are fixed in formalin, the fixation process thought cause signal suppression for lipid molecules. Studies have shown sections applied with...
White matter hyperintensities (WMH) occur in normal aging and across diagnostic categories of neurodegeneration. Ultra-high field imaging (UHF) MRI machines offer the potential to improve our understanding WMH. Post-mortem using UHF magnetic resonance (MRI) is a useful way assessing WMH, however, responsiveness UHF-MRI pathological changes within white has not been characterized. In this study we report post-mortem sequences aging, Alzheimer's disease, cerebrovascular disease. Seven Tesla...
Accurate and sensitive imaging biomarkers are required to study the progression of white matter (WM) inflammation in neurodegenerative diseases. Radioligands targeting translocator protein (TSPO) considered indicators neuroinflammation, but it is not clear how well expression TSPO coincides with major histocompatibility complex class II (MHCII) molecules WM. This aimed test ability detect activated WM microglia that immunohistochemically positive for MHCII rat models prodromal Alzheimer's...
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Periventricular white matter hyperintensities (pvWMHs) are a neurological feature detected with magnetic resonance imaging that clinically associated an increased risk of stroke and dementia. pvWMHs represent lesions characterized by regions myelin axon rarefaction as such likely involve changes in lipid composition; however, these alterations remain unknown. Lipids critical determining cell function survival. Perturbations expression have previously been disorders. Matrix-assisted laser...
Abstract Periventricular white matter hyperintensities (pvWMH) are neuroimaging abnormalities surrounding the lateral ventricles that apparent on magnetic resonance imaging (MRI). They associated with age, neurodegenerative disease, and cerebrovascular risk factors. While pvWMH ultimately represent a loss of structural integrity, pathological causes heterogeneous in nature, currently, cannot be distinguished using alone. could occur because combination small vessel disease (SVD), ependymal...
Traumatic brain injury is followed by a cascade of dynamic and complex events occurring at the cellular level. These include: diffuse axonal injury, neuronal cell death, blood-brain barrier break down, glial activation neuroinflammation, edema, ischemia, vascular energy failure, peripheral immune infiltration. The timing these post has been linked to severity functional outcome. Extracellular vesicles are membrane bound secretory that contain markers cargo pertaining their origin can cross...
Abstract Background Memory loss has been a central focus of Alzheimer’s disease (AD) research but executive dysfunction may be one the earliest indicators AD (Baudic et al., 2006). Executive function refers to group cognitive processes that are required for goal directed behaviours, such as working memory and flexibility. Amyloid‐β (Aβ) plaques neurofibrillary tangles considered pathological hallmarks AD, evidence suggests these not pathologies appear in brain (Sperling 2011). This...
Alzheimer’s disease (AD) is a chronic neurodegenerative condition affecting millions of people worldwide. With an aging population, it predicted that by 2050 the number individuals living with AD will triple, resulting in increased social and economic burden. Previous research has demonstrated pathological process leading to occurs years before positive diagnosis. Therefore, identifying biomarkers co‐occur within early stage progression predict worsening cognitive outcomes are critically...
Abstract Background Currently there are no widely accepted diagnostic tests for Alzheimer’s disease (AD). Although is much excitement in the field of biomarker development, most proposed AD expensive, require highly specialized sample handling, invasive approaches or radiation exposure. The goal this project was to develop non‐invasive assessment using nanoflow cytometry examine proteins on circulating brain‐derived extracellular vesicles plasma. Method Plasma samples were collected from a...
Abstract Background Atrial fibrillation (AF) increases risk of cognitive decline independent stroke, but mechanisms remain unclear. Elevated levels peripheral inflammatory markers common in AF are associated with increased blood‐brain barrier (BBB) permeability and may contribute to neuroinflammation degeneration. To examine the role these dementia risk, we estimated associations plasma C‐reactive protein (CRP), interleukin‐6 (IL‐6), fibrinogen aging adults without AF. Method Dementia free...
Cerebral small vessel disease (SVD) affects the microvasculature of brain, increasing in prevalence with age and associating cognitive decline dementia. SVD can be visualized on MR imaging by markers such as hyperintensities within white matter (WMH). Periventricular WMH frontal lobe specifically may have a negative effect executive functions. Executive dysfunction represents set changes, including processing speed attention, that precede memory impairment Alzheimer disease. The etiology...