IgA nephropathy, a primary glomerulonephritis, is principally characterized by mesangial deposits of immune complexes. Recently, it has been demonstrated that cultured glomerular cells (MC) express Fc alpha and gamma receptors. In this work, we studied whether the interaction soluble aggregates IgG (AIgA AIgG) with MC triggers number responses, including generation release inflammatory mediators, cell proliferation, catabolism Aggregates bound to were catabolized in time-dependent manner....

Cardiac interventional procedures are often performed under fluoroscopic guidance, exposing both the patient and operators to ionizing radiation. To reduce this risk of radiation exposure, we exploring use photoacoustic imaging paired with robotic visual servoing for cardiac catheter visualization surgical guidance. A catheterization procedure was on two in vivo swine after inserting an optical fiber into produce signals from tip fiber-catheter pair. combination employed visualize maintain...

Abstract Previous works have demonstrated that mesangial cells (MC) possess Fc receptors for IgG. We investigated whether serum IgA could bind to MC in culture through a specific receptor. The binding of 125I-IgA (4 degrees C, 60 min) was dose dependent and saturable, the Scatchard analysis revealed population 1 x 10(5) sites per cell with Ka 7.8 10(8) M-1. This receptor because unlabeled its fragment were able inhibit binding, whereas IgG, IgM, F(ab) not. Parallel experiments showed human...

Deep fully connected networks are often considered "universal approximators" that capable of learning any function. In this article, we utilize particular property deep neural (DNNs) to estimate normalized cross correlation as a function spatial lag (i.e., coherence functions) for applications in coherence-based beamforming, specifically short-lag (SLSC) beamforming. We detail the composition, assess performance, and evaluate computational efficiency CohereNet, our custom DNN, which was...

Mobile electrocardiogram (ECG) monitoring is an emerging area that has received increasing attention in recent years, but still real-life validation for elderly residing low and middle-income countries scarce. We developed a wearable ECG monitor integrated with self-designed wireless sensor signal acquisition. It used native purposely designed smartphone application, based on machine learning techniques, automated classification of captured beats from aged people. When tested 100 older...

Abdominal surgeries carry considerable risk of gastrointestinal and intra-abdominal hemorrhage, which could possibly cause patient death. Photoacoustic imaging is one solution to overcome this challenge by providing visualization major blood vessels during surgery. We investigate the feasibility <italic>in vivo</italic> vessel for photoacoustic-guided liver pancreas surgeries. <italic>In photoacoustic in these two abdominal organs was successfully achieved after a laparotomy performed on...

Spinal fusion surgeries require accurate placement of pedicle screws in anatomic corridors without breaching bone boundaries. We are developing a combined ultrasound and photoacoustic image guidance system to avoid screw misplacement accidental breaches, which can lead nerve damage.Pedicle cannulation was performed on human cadaver, with co-registered images acquired at various time points during the procedure. Bony landmarks obtained from coherence-based lumbar vertebrae were registered...

Significance: Photoacoustic-based visual servoing is a promising technique for surgical tool tip tracking and automated visualization of photoacoustic targets during interventional procedures. However, one outstanding challenge has been the reliability obtaining segmentations using low-energy light sources that operate within existing laser safety limits. Aim: We developed first known graphical processing unit (GPU)-based real-time implementation short-lag spatial coherence (SLSC)...

The successful integration of computer vision, robotic actuation, and photoacoustic imaging to find follow targets interest during surgical interventional procedures requires accurate target detectability. This detectability has traditionally been assessed with image quality metrics, such as contrast, contrast-to-noise ratio, signal-to-noise ratio (SNR). However, predicting tracking performance expectations when using these traditional metrics is difficult due unbounded values sensitivity...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe existence of second-row anomeric interactions. Conformational analysis 2-substituted 5-methyl-5-aza-1,3-dithiacyclohexanesEusebio Juaristi, Eduardo A. Gonzalez, B. Mario Pinto, Blair D. Johnston, and Ruby NagelkerkeCite this: J. Am. Chem. Soc. 1989, 111, 17, 6745–6749Publication Date (Print):August 1, 1989Publication History Published online1 May 2002Published inissue 1 August...

Spectral unmixing techniques for photoacoustic images are often used to isolate signal origins (e.g., blood, contrast agents, lipids). However, these require many 12–59) wavelength transmissions optimal performance exploit the optical properties of different biological chromophores. Analysis acoustic frequency response signals has potential provide additional discrimination from materials, with added benefit potentially requiring only a few emissions. This study presents our initial results...

Imaging of musculoskeletal tissue dynamics is currently an exploratory field with the goal aiding rehabilitation and performance evaluation pathological or asymptomatic patients. In this pilot study, initial elasticity assessments biceps brachii were conducted in a novel crawling wave sonoelastography (CWS) system implemented on research ultrasound instrument graphical processing unit capabilities, displaying quantitative values at 4 frames per second. The CWS computes stiffness generation...

Many cardiac interventional procedures (e.g., radiofrequency ablation) require fluoroscopy to navigate catheters in veins toward the heart. However, this image guidance method lacks depth information and increases risks of radiation exposure for both patients operators. To overcome these challenges, we developed a robotic visual servoing system that maintains visualization segmented photoacoustic signals from catheter tip. This was tested two vivo catheterization with ground truth position...

The process of registering ultrasound (US)images to computed tomography (CT)images relies on accurate segmentation bony structures in US images. However, images often suffers from the presence speckle noise, clutter, and acoustic shadowing. We propose improve bone with a novel Locally Weighted SLSC (LW-SLSC)beamforming method, which is based minimization total variation spatial coherence weighted sum. Application this beamformer an ex vivo human vertebra resulted 911% contrast-to-noise ratio...

Crawling wave sonoelastography (CWS) provides quantitative stiffness information from an examined tissue by the application of two mechanical vibrations that generates interference pattern which is analyzed to reconstruct a shear speed (SWS) image. While CWS performance using parallel excitation setup (PES) has been widely studied in literature, its implementation complicated apply (i.e. breast, liver) technique human often requires normal setup. The aim this study validate SWS estimation...

Harmonics-to-noise ratios (HNRs) are affected by general aperiodicity in voiced speech signals. To specifically reflect a signal-to-additive-noise ratio, the measurement should be insensitive to other periodicity perturbations, like jitter, shimmer, and waveform variability. The ensemble averaging technique is time-domain method which has been gradually refined terms of its sensitivity jitter variability required number pulses. In this paper, shimmer introduced model average, formula derived...

A conceptual analysis of the coupling between bars and infragravity waves is performed combining laboratory experiments numerical modeling. Experiments are carried out in a wave flume with barred profile. The Boussinesq fully-nonlinear model SERR1D validated data sensitivity next to study influence on dynamics bar amplitude location, swash zone slope. novel technique incident reflected motions separation that conserves temporal characteristics applied. We observe changing induces substantial...

Short-lag spatial coherence (SLSC) beamforming has the potential to improve diagnostic power of a multitude ultrasound imaging techniques. One challenge for advanced real-time implementation is repeated correlation calculations. To address this challenge, paper introduces CohereNet - novel deep neural network architecture that estimates function in efforts bypass calculations required SLSC imaging. The was trained and evaluated using vivo breast data, demonstrating similar contrast, CNR,...