A5084921166- Hepatitis B Virus Studies
- HIV Research and Treatment
- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- DNA Repair Mechanisms
- Animal Virus Infections Studies
Indiana University – Purdue University Indianapolis
2017-2025
Indiana University School of Medicine
2017-2025
UPMC Hillman Cancer Center
2020-2025
Hepadnavirus covalently closed circular (ccc) DNA is the bona fide viral transcription template, which plays a pivotal role in infection and persistence. Upon infection, non-replicative cccDNA converted from incoming de novo synthesized genomic relaxed (rc) DNA, presumably through employment of host cell's repair mechanisms nucleus. The conversion rcDNA into requires preparation extremities at nick/gap regions for strand ligation. After screening 107 cellular genes, we herein report that...
Hepatitis B virus (HBV) relaxed circular DNA (rcDNA) possesses an 8–9 nucleotide-long terminal redundancy (TR, or r ) on the negative (-) strand derived from reverse transcription of viral pregenomic RNA (pgRNA). It remains unclear whether TR forms a 5’ 3’ flap structure HBV rcDNA and which copy is removed during covalently closed (cccDNA) formation. To address these questions, mutant cell line HepDES-C1822G was established with C1822G mutation in pgRNA coding sequence, altering sequence...
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), serving as the viral persistence form and transcription template of HBV infection, hijacks host histone non-histone proteins to a minichromosome utilizes posttranslational modifications (PTMs) "histone code" for its transcriptional regulation. X protein (HBx) is known cccDNA activator. In this study we established dual system inducible reporter cell lines modelling infection with wildtype (wt) HBx-null HBV, both secreting...
The biosynthesis of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) requires the removal linked viral polymerase from 5' end minus strand [(-)strand] relaxed (rcDNA), which generates a deproteinated rcDNA (DP-rcDNA) intermediate. In present study, we systematically characterized four termini cytoplasmic HBV DP-rcDNA by 5'/3' rapid amplification cDNA ends (RACE), radiolabeling, and exonuclease digestion, revealed following observations: (i) possess an identical 3' (-)strand...
Serving as a key viral factor for chronic hepatitis B virus (HBV) infection, HBV covalently closed circular DNA (cccDNA) is formed in the cell nucleus from relaxed (rcDNA) by hijacking host repair machinery. Previous studies have identified several factors involved cccDNA formation through hypothesis-driven research with some help RNA interference (RNAi) screening and/or biochemistry approaches.