A5100718819- Hepatitis B Virus Studies
- Hepatitis C virus research
- HIV Research and Treatment
- Bone fractures and treatments
- HIV/AIDS drug development and treatment
- Orthopedic Infections and Treatments
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Orthopaedic implants and arthroplasty
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Liver Disease Diagnosis and Treatment
- Lipid Membrane Structure and Behavior
- DNA Repair Mechanisms
- Viral Infections and Outbreaks Research
- COVID-19 Impact on Reproduction
- Neonatal Health and Biochemistry
- Peptidase Inhibition and Analysis
- Cytomegalovirus and herpesvirus research
- Cholesterol and Lipid Metabolism
- Bacteriophages and microbial interactions
- Transportation Safety and Impact Analysis
- Protein Degradation and Inhibitors
- Bone Tissue Engineering Materials
Indiana University – Purdue University Indianapolis
2017-2025
Indiana University School of Medicine
2017-2025
UPMC Hillman Cancer Center
2020-2025
University of Pittsburgh
2020-2024
Army Medical University
2023
University of Pittsburgh Medical Center
2022
Shandong Provincial QianFoShan Hospital
2014
Shandong University
2014
Emory University
2011
Significance The novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the etiological agent of coronavirus disease-2019 (COVID-19), has swept world in unprecedented speed. In a few months, SARS-CoV-2 infected millions people and caused tens thousands deaths. There are no Food Drug Administration-approved antivirals or vaccines yet available clinical treatments limited to supportive therapies that help alleviate symptoms. Thus, there is an urgent need identify effective as...
Hepadnavirus covalently closed circular (ccc) DNA is the bona fide viral transcription template, which plays a pivotal role in infection and persistence. Upon infection, non-replicative cccDNA converted from incoming de novo synthesized genomic relaxed (rc) DNA, presumably through employment of host cell's repair mechanisms nucleus. The conversion rcDNA into requires preparation extremities at nick/gap regions for strand ligation. After screening 107 cellular genes, we herein report that...
HBV is an enveloped DNA virus that replicates its genome via reverse transcription of a pregenomic (pg) RNA intermediate in hepatocytes. Interestingly, can be detected virus-like particles chronic hepatitis B (CHB) patient serum and has been utilized as biomarker for intrahepatic cccDNA activity treated patients. However, the biogenesis molecular characteristics remain to fully defined. In this study, we found encapsidated predominately consists pgRNA, which are detergent-...
Infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a wide spectrum of syndromes involving multiple organ systems and is primarily mediated viral spike (S) glycoprotein through the receptor-binding domain (RBD) numerous cellular proteins including ACE2, transmembrane serine protease (TMPRSS2), kidney injury molecule-1 (Kim-1), neuropilin-1 (NRP-1). In this study, we examined entry tropism SARS-CoV-2 SARS-CoV using S protein-based pseudoviruses to infect 22 cell...
Hepatitis B virus (HBV) relaxed circular DNA (rcDNA) possesses an 8–9 nucleotide-long terminal redundancy (TR, or r ) on the negative (-) strand derived from reverse transcription of viral pregenomic RNA (pgRNA). It remains unclear whether TR forms a 5’ 3’ flap structure HBV rcDNA and which copy is removed during covalently closed (cccDNA) formation. To address these questions, mutant cell line HepDES-C1822G was established with C1822G mutation in pgRNA coding sequence, altering sequence...
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), serving as the viral persistence form and transcription template of HBV infection, hijacks host histone non-histone proteins to a minichromosome utilizes posttranslational modifications (PTMs) "histone code" for its transcriptional regulation. X protein (HBx) is known cccDNA activator. In this study we established dual system inducible reporter cell lines modelling infection with wildtype (wt) HBx-null HBV, both secreting...
Rheumatoid arthritis (RA) is characterized by a chronic inflammatory process that targets the synovial lining of diarthrodial joints. TIM-3 plays key role in negative regulation immune response. In this study, we investigated expression on CD4+ and CD8+ T cells from systemic (peripheral blood) local (synovial fluid) perspectives RA. Level TIM-3+ peripheral blood fluid patients as well healthy controls was measured flow cytometry. Results showed significantly increased both RA (p < 0.001 p...
ABSTRACT Chronic hepatitis B virus (HBV) infection remains a significant public health burden with no cure currently available. The research to HBV has long been hampered by the lack of immunocompetent small animal models capable supporting infection. Here, we set out explore feasibility golden Syrian hamster as an rodent model for We first started in vitro assessments replication cycle primary hepatocytes (PHaHs) adenoviral (Ad-HBV) transduction. Our results demonstrated that PHaHs support...
The biosynthesis of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) requires the removal linked viral polymerase from 5' end minus strand [(-)strand] relaxed (rcDNA), which generates a deproteinated rcDNA (DP-rcDNA) intermediate. In present study, we systematically characterized four termini cytoplasmic HBV DP-rcDNA by 5'/3' rapid amplification cDNA ends (RACE), radiolabeling, and exonuclease digestion, revealed following observations: (i) possess an identical 3' (-)strand...
Hepatitis B virus (HBV) chronic infection, a long-studied but yet incurable disease, remains major public health concern worldwide. Given that HBV replication cycle highly depends on host factors, deepening our understanding of the host-virus interaction is thus great significance in journey finding cure.
Serving as a key viral factor for chronic hepatitis B virus (HBV) infection, HBV covalently closed circular DNA (cccDNA) is formed in the cell nucleus from relaxed (rcDNA) by hijacking host repair machinery. Previous studies have identified several factors involved cccDNA formation through hypothesis-driven research with some help RNA interference (RNAi) screening and/or biochemistry approaches.
Abstract Background To evaluate the effectiveness of a sequential internal fixation strategy and intramedullary nailing with plate augmentation (IMN/PA) for bone reconstruction in management infected femoral shaft defects using Masquelet technique. Methods We performed retrospective descriptive cohort study 21 patients (mean age, 36.4 years) treated by technique minimum follow-up 18 months after second stage. After aggressive debridement, temporary stabilisation (T1) was achieved an...
To compare the effect of internal fixation vs. external after debridement in stage I Masquelet technique for Cierny-Mader (C-M) type Ⅳ chronic post-traumatic tibial osteomyelitis.This retrospective observational study included patients with osteomyelitis who underwent staged treatment between January 2016 and June 2020 at 920 Hospital Joint Logistic Support Force PLA. The were grouped according to they received I. Infection recurrence, time radiological bone healing full weight-bearing,...
SARS-CoV-2 NSP12, the viral RNA-dependent RNA polymerase (RdRp), is required for replication and a therapeutic target to treat COVID-19. To facilitate research on NSP12 protein, we developed rat monoclonal antibody (CM12.1) against N-terminus that can functional studies. Immunoblotting immunofluorescence assay (IFA) confirmed specific detection of protein by this cells overexpressing protein. Although generated from ORF1ab polyprotein, IFA human autopsy COVID-19 lung samples revealed...
Abstract Occupant Protection System (OPS) is a key component in today's vehicle systems to reduce occupant's injuries during collisions. To assess OPS performance under wide variety of crash conditions, barrier tests, laboratory dynamic sled tests and computer simulations are the major tools for development. As main input system, pulse controls design. Its variations have significant impact on overall system responses. A well-correlated MADYMO model employed evaluate effects frontal OPS. set...
<title>Abstract</title> Introduction: Masquelet induced membrane surgery is a viable option for the reconstruction of extensive bone defects. This study aimed to comprehensively compare clinical efficacy 3D printed microporous tantalum prosthesis and autologous graft in final stage during treatment lower extremity osteomyelitis with large segmental defect. Methods A prospective analysis was conducted on 43 patients defect caused by treated surgery. Among these, 21 were implanted (Prosthesis...
Tripartite motif (TRIM) proteins, comprising a family of over 100 members with conserved motifs, exhibit diverse biological functions. Several TRIM proteins influence viral infections through direct antiviral mechanisms or by regulating host innate immune responses. To identify modulating hepatitis B virus (HBV) replication, we assessed 45 human TRIMs in HBV-transfected HepG2 cells. Our study revealed that ectopic expression 12 significantly reduced HBV RNA and subsequent capsid-associated...
Hepatitis B virus (HBV) exploits the endosomal sorting complexes required for transport (ESCRT)/multivesicular body (MVB) pathway virion budding. In addition to enveloped virions, HBV-replicating cells nonlytically release non-enveloped (naked) capsids independent of integral ESCRT machinery, but exact secretory mechanism remains elusive. Here, we provide more detailed information about existence and characteristics naked capsid, as well viral host regulations capsid egress. HBV capsid/core...
Abstract Objective In this study, we aimed to compare the outcomes of two-stage induced membrane technique (IMT) and one-stage autografting in treatment aseptic atrophic nonunion lower limb long bones. Methods From January 2014 2022, reviewed all surgically treated bone patients, including patients aged 18 years or older with nonunion, who were either autografting. Outcome parameters interns clinical, quality life healthcare burden recorded retrospectively analysed between two populations....
β-D-3′-Azido-2′,3′-dideoxyguanosine (3′-azido-ddG) is a potent inhibitor of HIV-1 replication with superior resistance profile to zidovudine. Recently, we identified five novel 6-modified-3′-azido-ddG analogs that exhibit similar or anti-HIV-1 activity compared 3′-azido-ddG in primary cells. To gain insight into their structure–activity–resistance relationships, synthesized triphosphate (TP) forms and assessed ability inhibit reverse transcriptase (RT). Steady-state pre-steady-state kinetic...