Self-assembly of solid organs from single cells would greatly expand applicability regenerative medicine.Stem/progenitor can self-organize into micro-sized organ units, termed organoids, partially modelling tissue function and regeneration.Here we demonstrated 3D self-assembly adult induced pluripotent stem cell (iPSC)-derived fibroblasts, keratinocytes endothelial progenitors both, planar human skin in vivo a novel type spheroid-shaped organoids vitro, under the aegis platelet...

Intravascular transplantation of tissue factor (TF)-bearing cells elicits an instant blood-mediated inflammatory reaction (IBMIR) resulting in thrombotic complications and reduced engraftment.Here we studied the hemocompatibility commonly used human white adipose (WAT), umbilical cord (UC) bone marrow stromal (BMSC) devised a possible strategy for safe efficient cell transplantation.Methods: Stromal identity, purity, TF expression was tested by RTQ-PCR, flow cytometry immunohistochemistry....

Background: With the increasing demand for autologous breast reconstruction, different surgical techniques have emerged to provide patients with best possible result tailored their individual needs. The upper thigh provides an alternative tissue resource in where abdominal based flaps are not feasible. Although outcomes and donor-site morbidity demonstrate favorable results using thigh-based free differences skin constitution color between two flap donor sites been investigated. Methods:...

Placebo controlled double-blinded, prospectively randomized clinical trial with 17 patients (11 verum, 5 placebo) for evaluation of cellulite treatment Acoustic Wave Therapy, (AWT®) was performed.The were treated once a week 7 weeks, total 8 treatments the D-ACTOR® 200 by Storz Medical AG. Data collected at baseline, before 8th treatment, 1 month (follow-up 1) and 3 months 2) after last patients' questionnaire, weight control, measurement circumference standardized photography. Treatment...

Background and Objectives Laser hair removal is the most common laser therapy third commonly performed procedure with more than one million treatments in United States 2016. This retrospective study was conducted to assess long‐term efficacy safety of 755 nm for removal. Study Design/Materials Methods Nearly, 3,606 were long‐pulsed wavelength equipped an epidermal cooling device between 1997 2005 followed till 2013. Standardized assessments by two treating physicians patients at follow‐up...

Background: Happy 30th birthday to the transverse myocutaneous gracilis (TMG) flap. Since 1991 TMG flap has been used reconstruct a wide variety of defects and became workhorse reliable alternative deep inferior epigastric perforator (DIEP) in many breast reconstruction services worldwide. This manuscript sheds light on history success by critically reviewing present literature series 300 patients receiving reconstruction. Patients Methods: The was reviewed retrospective double center cohort...

Background: In secondary autologous breast reconstruction, the current standard is a flap derived from lower abdomen or back. If these donor sites are not available because of lack tissue, prior operations, simply patient's desire to avoid sites, authors use transverse musculocutaneous gracilis if feasible. Methods: The retrospectively evaluated only patients where reconstruction was performed with mentioned reasons. Indications, limitations, advantages, and technique discussed by sharing...

Antibodies directed to cyclic citrullinated peptides (anti-CCP) are highly specific for rheumatoid arthritis (RA) and can easily be detected in sera by using commercially available immunoassays. The second version of the anti-CCP test (anti-CCP2) demonstrated high specificity (89-98%) good sensitivity (41-88%) RA. Commercially ELISA methods from three different companies on market. All CCP2 assays show similar results as all use same antigen-coated plates. This study was an evaluation a new...

Meralgia paresthetica (MP) is a rare lateral femoral cutaneous nerve-(LFCN)-mononeuropathy. Treatment for this disorder includes conservative and operative approaches; the latter considered if therapy fails. The most commonly used surgical approaches are decompression/neurolysis avulsion/neurectomy. However, there no definitive guidelines on optimal approach to be used. purpose of study was evaluate outcome decompression LFCN treatment persistent MP with preservation sensation along...

The peroneus brevis flap can be used as either proximally or distally based for coverage of small to medium‐sized defects in the lower leg. purpose this study was clarify vascular anatomy muscle. An anatomical dissection performed on 17 fixed adult cadaver legs. Altogether, 87 segmental branches (mean 5.1 ± 1.6 per leg) from fibular anterior tibial artery muscle were identified. Sixty‐two 3.4 1.1 artery), whereas 25 1.4 0.9 artery) originated artery. distance between most distal branch and...

Background and Objective Skin changes are among the most visible signs of aging. Fractional ablative lasers improve skin quality by making small wounds that heal rapidly without scarring. While they texture discoloration, there is minimal effect on laxity. This study was performed to assess shrinkage removing multiple full‐thickness columns with coring needles combined wound closure. Materials Methods In 5 swine 116 squares (3 cm 2 ) were demarcated for treatment control sites. sites 10%...

Summary: The deep inferior epigastric perforator (DIEP) flap is the gold standard for autologous breast reconstruction. One downside its risk of postsurgical herniation and bulging due to opening rectus fascia intramuscular dissection abdominis muscle. Additionally this part surgery very time consuming. While superficial artery (SIEA) has been thoroughly described total reconstruction, use circumflex iliac in field limited. This series introduces an extended (SCIA) nourished solely by...

Abstract Application of in vitro transcribed (IVT) messenger ribonucleic acid (mRNA) is an increasingly popular strategy to transiently produce proteins as therapeutics a tissue or organ choice. Here, we focused on the skin and aimed test if whole human explant technology can be used evaluate expression efficacy different IVT Interferon alpha (IFN-α) mRNA constructs situ , after biolistic delivery. Skin explants were viable intact for at least five days based histologic analysis TUNEL...

In patients with rheumatoid arthritis (RA), overwhelming inflammatory activity and immune activation are indicated by elevated concentrations of markers such as erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), neopterin. Furthermore, accelerated tryptophan (Trp) degradation the enzyme indoleamine 2,3-dioxygenase (IDO) is detectable in blood samples an increased kynurenine (Kyn) to Trp ratio (Kyn/Trp). This study comprises 22 (20 women, 2 men) long-standing, moderate severe RA,...

Background: Proximal radial nerve lesions located between the brachial plexus and its division into superficial deep branches are rare but severe injuries. The majority of these occur in association with humerus fractures, directly during trauma or later osteosynthesis for fracture treatment. Diagnostics surgical interventions often delayed. best type treatment outcome to be expected is uncertain. Methods: Twelve patients proximal due prior surgery were included this study underwent...

Rheumatoid arthritis is a systemic autoimmune disease characterized by joint erosions, progressive focal bone loss, and chronic inflammation.20 female patients with moderate-to-severe rheumatoid were treated anti-TNF-antibody adalimumab in addition to concomitant antirheumatic therapies. Patients assessed for overall activity using the DAS28 score, neopterin, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) concentrations as well osteoprotegerin (OPG) soluble receptor activator...

Ablative fractional laser treatment uses thousands of very small beam wounds to damage a fraction the skin, which stimulates tissue remodeling. Each open micro-wound heals without scarring, but amount skin tightening achieved is limited. This animal study was performed test hypothesis that immediate temporary closure could increase after ablative treatment.Four adult swine were used for study; 98 square sites (3 × 3 cm) tattooed on abdomen and flanks each pig. An Erbium:YAG (Sciton Profile,...

Meralgia paraesthetica is a mononeuropathy of the lateral femoral cutaneous nerve. According to literature, nerve travels beneath inguinal ligament 1.3 5.1 cm medial anterior superior iliac spine. Compression at this site may cause pain and paresthesia. The aim study was provide more accurate measurements improve diagnostic surgical management meralgia paraesthetica.The dissected bilaterally in 50 Thiel-embalmed human cadavers. Measurements were performed with standard caliper inferior...

Introduction: The transverse myocutaneous gracilis (TMG) flap has become a popular and reliable alternative for autologous breast reconstruction. Initially described as valuable tissue source women with low body-mass index, indications nowadays have widely expanded. Western civilization demographic development its aging population the steady growing average BMI led to increasing reconstructions TMG flaps in overweight aged individuals. Patients Methods: A total of 300 free unilateral...