- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Catalysis and Immobilization
- Phytase and its Applications
- Biofuel production and bioconversion
- Identification and Quantification in Food
- Microbial Metabolic Engineering and Bioproduction
- Yersinia bacterium, plague, ectoparasites research
- Proteins in Food Systems
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Phosphorus and nutrient management
- Membrane Separation Technologies
- Aquaculture Nutrition and Growth
- Nanoparticle-Based Drug Delivery
- Food composition and properties
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Transgenic Plants and Applications
- Layered Double Hydroxides Synthesis and Applications
- Invertebrate Immune Response Mechanisms
- Anaerobic Digestion and Biogas Production
- Marine Sponges and Natural Products
Chinese Academy of Fishery Sciences
2011-2025
Donghua University
2020-2024
Yunnan Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2018-2021
Bioscience (China)
2021
Pingdingshan University
2015
Ocean University of China
2006-2008
Beihang University
2004
University of Shanghai for Science and Technology
2004
The first luciferase from Antarctic krill (LAK) was cloned and successfully expressed in Escherichia coli BL21(DE3). LAK exhibits the unique ability to emit bright violet fluorescence at an emission wavelength of 350 nm, which represents lowest reported bioluminescence for luciferases. However, its low thermal stability poses a limitation broader application. In this study, we employed rational design approach introduce three pairs artificial disulfide bonds into LAK. Circular dichroism (CD)...
To improve the reusability and organic solvent tolerance of microbial lipase expand application (hydrolysis, esterification, transesterification), we immobilized marine using different methods determined properties lipases. Considering activity cost lipase, concentration was fixed at 2 mg/mL. The optimal temperature lipases 40°C 5°C higher than free lipase. activities were much alkaline pH (more 50% 12). lost most (35.3%) retained more 46.4% their initial after 3 h heat treatment 70°C. At...
Abstract Two metal coordination polymers, {[Zn(bpdc)(bip)]·2H2O}n (1) and [Zn(tdc)(bip)] (2) [H2bpdc = biphenyl-4,4’-dicarboxylate, H2tdc thiophene-2,5-dicarboxylate, bip 3,5-bis(imidazole-1-yl)pyridine], have been synthesized characterized by IR, elemental analysis, XRD, X-ray single-crystal diffraction. In 1, bpdc link Zn(II) ions into a corrugated 2D layer. The layers polycatenate each other, yielding 2D→3D polycatenation net. 2, dinuclear units are formed further connected tdc to...