The objective of this study was to elucidate the protective role quercetin in atherosclerosis by examining its effect on phenotypic switch vascular smooth muscle cells (VSMCs) macrophage-like and underlying regulatory pathways. Aorta tissues from apolipoprotein E-deficient (ApoE KO) mice fed a high-fat diet (HFD), treated with or without 100 mg/kg/day quercetin, were analyzed for histopathological changes molecular mechanisms. Quercetin found decrease size atherosclerotic lesions mitigate...

Objective: To explore the mechanism of action “Weilingxian-Guizhi” two drugs in treatment gout. Method: First obtain 15 chemical components contained drug, predict its target points through database, and then construct gout-related protein-protein interaction network (PPI), “Weiling,” “Xian, Guizhi” drug pair “active ingredient-predicted target” network, construction ingredient-potential based on Kyoto Encyclopedia Genes Genomes (KEGG) biological pathway enrichment analysis gene ontology...

Background: Vascular smooth muscle cells (VSMCs) senescence exacerbates vascular diseases like atherosclerosis and hypertension. Angiotensin II (Ang II) is a strong inducer of VSMCs senescence, causing damage, though its exact mechanism unclear. Nicotinamide mononucleotide (NMN), NAD+ precursor, has gained attention for anti-senescence potential, yet role in inhibiting not fully understood. Methods: This study assessed markers, including β-galactosidase activity (SA-β-gal) the...

Klotho is an aging-related marker closely associated with a number of diseases. A growing body evidence suggests that dietary factors and lifestyle habits can impact serum levels. The effect fiber, key component healthy diet, on the body's levels has not been fully elucidated.The aim this study was to explore relationship between fiber intake in people aged 40-79 years United States.A total 11,282 participants were included study, all from National Health Nutrition Examination Survey 2007...

Background: The relationship between dietary carbohydrate intake and serum Klotho levels, an aging biomarker, remains uncertain. Objective: This study aimed to investigate the association levels among American adults aged 40–79. Methods: We analyzed data from 10,669 40–79 years who participated in National Health Nutrition Examination Survey (NHANES) 2007 2016. Trained interviewers assessed using a 24 h recall. Serum concentrations were measured commercially available ELISA kits provided by...

Resveratrol has been shown to improve insulin resistance via activating the NAD+ -dependent deacetylase SIRT1, but effects of resveratrol on ethanol-induced remain unclear. This study was designed explore potential mechanism by which ameliorated resistance, focusing its regulations ratio /NADH and SIRT1 expression.Male Sprague-Dawley rats were fed either control or ethanol liquid diets containing 0.8, 1.6 2.4 g/kg·bw with without 100 mg/kg·bw for 22 weeks. improved (2.4 g/kg·bw) induced...

Epoxidation at the allylic side chain is a major metabolic pathway for allylbenzene and its naturally occurring analogs safrole, estragole, eugenol. We demonstrate herein that epoxide metabolites of allylbenzene, safrole can form covalent adducts with DNA in vitro, binding primarily to guanine, but also other three bases. Epoxide hydrolases prevent 2',3'-oxide vitro. Four distinct were detected by analytical TLC after reaction 2'-deoxyguanosine 2',3'-oxide. One unstable adduct was formed...

Background: There is increasing evidence suggesting that serum neurofilament light chain (sNfL) levels can be used as biomarkers for axonal injury. Retinol recognized its significant involvement in nervous system function, but the precise connection between dietary retinol and sNfL remains uncertain. Objective: Our objective was to investigate relationship intake sNfL, find an optimal level neurological health. Methods: In National Health Nutrition Examination Survey (NHANES), conducted from...

As the sole producers of insulin under physiological conditions, normal functioning pancreatic β cells is crucial for maintaining glucose homeostasis in body. Due to high oxygen and energy demands required secretion, hypoxia has been shown play a critical role β-cell dysfunction. Lipid metabolism abnormalities, common metabolic feature type 2 diabetic patients, are often accompanied by tissue caused overload lead increased free fatty acid (FFA) levels. However, specific mechanisms underlying...

2',3'-Allylic epoxide derivatives of allylbenzene and its analogs estragole, eugenol, safrole were synthesized, their enzymatic conversion to dihydrodiols by cytosolic microsomal hydrolases was examined. All four epoxides good substrates for both hydrolases, with Michaelis constants in the low micromolar range. Two putatively selective inhibitors trichloropropylene oxide nordihydroguaiaretic acid, used inhibit hydrolysis these allylic epoxides. Minimal selectivity toward either hydrolase...

Diabetic nephropathy (DN) is one of the most important renal complications associated with diabetes, and mechanisms are yet to be fully understood. To date, few studies have shown antioxidant effects 1α,25-dihydroxyvitamin-D 3 [1,25(OH) 2 D ] on hyperglycemia-induced injury. The aim present study was explore potential mechanism by which 1,25(OH) reduced oxidative stress in diabetic rat kidneys. In this study, we established a vitamin D-deficient spontaneous diabetes model: 5–6 wk age Zucker...

Abstract Introduction Alzheimer's disease (AD), a common form of dementia, has been reported to influence 27 million individuals globally. Several risk factors including oxidative stress, gut microbiota imbalance, and cognitive activity are be closely associated with the initiation or progression AD. Although miR‐483‐3p was identified downregulated in AD patient serum. However, biological role mechanism remained unknown Here, we explored Methods Sprague–Dawley rats were injected homocysteine...

Ebselen is an organoselenium compound that has demonstrated potent antioxidant and anti-inflammatory effects in previous studies. The present study was conducted to evaluate the effect of ebselen on myocardial ischemia-reperfusion (I/R) injury a rat model elucidate related mechanisms. Myocardial infarct size assessed using triphenyltetrazolium chloride staining. evaluated according histopathological ultrastructural alterations hearts serum activity levels cardiac enzymes, including creatine...

The metabolism of allylbenzene 2',3'-oxide, estragole and was studied in the isolated perfused rat liver. Formation dihydrodiol glutathione conjugate metabolites detected for both epoxides presence after perfusion or indicated formation allylic epoxide intermediates intact A comparison elimination kinetics parent compounds that were relatively rapidly detoxified probably do not accumulate on vivo. Acute toxicity epoxides, measured as release alanine aminotransferase activity into perfusate,...