A5057717794- Glioma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Telemedicine and Telehealth Implementation
- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- Mobile Health and mHealth Applications
- Hepatitis B Virus Studies
- Biochemical and Molecular Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Protein Degradation and Inhibitors
- Lung Cancer Research Studies
- Acute Myeloid Leukemia Research
- Neurofibromatosis and Schwannoma Cases
- Cardiac Arrhythmias and Treatments
- Peptidase Inhibition and Analysis
- Social Media in Health Education
- Neuroendocrine Tumor Research Advances
- COVID-19 and Mental Health
- Liver Disease Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Single-cell and spatial transcriptomics
- Carbohydrate Chemistry and Synthesis
- Healthcare Systems and Public Health
- Workplace Health and Well-being
- Asthma and respiratory diseases
NGM Biopharmaceuticals (United States)
2022-2024
McGill University
2022-2024
AbbVie (United States)
2017-2020
Roche (United States)
2019
University of British Columbia
2012-2019
Canada's Michael Smith Genome Sciences Centre
2018-2019
Merck & Co., Inc., Rahway, NJ, USA (United States)
2018
Hospital Clínico de la Universidad de Chile
2018
AbCellera (Canada)
2017
University of Chile
2017
Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, open source computational methods. Using we generated resource 51,926 matched cell images diverse types including lines, xenografts, diagnostic samples with limited...
Abstract BRAF genomic alterations are the most common oncogenic drivers in pediatric low-grade glioma (pLGG). Arm 1 ( n = 77) of ongoing phase 2 FIREFLY-1 (PNOC026) trial investigated efficacy oral, selective, central nervous system–penetrant, type II RAF inhibitor tovorafenib (420 mg m − once weekly; 600 maximum) patients with -altered, relapsed/refractory pLGG. 60) is an extension cohort, which provided treatment access for -altered pLGG after arm closure. Based on independent review,...
Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined reconstituted entire life cycle in nonhepatic 293T cells. This study shows robust entry, RNA replication, and production infectious cells highlights key required for liver tropism HCV.
Here, we describe the design, synthesis, biological evaluation, and identification of a clinical candidate non-nucleoside reverse transcriptase inhibitors (NNRTIs) with novel aryl-phospho-indole (APhI) scaffold. NNRTIs are recommended components highly active antiretroviral therapy (HAART) for treatment HIV-1. Since major problem associated NNRTI is emergence drug resistant virus, this work focused on optimization APhI against clinically relevant HIV-1 Y181C K103N mutants Y181C/K103N double...
As technology will continue to play a pivotal role in modern-day health care and given the potential impact on nursing profession, it is vitally important examine types features of digital education so that graduates are better equipped with necessary knowledge skills needed provide safe quality keep abreast rapidly evolving technological revolution.
Up to 44% of ankle fractures have involvement the posterior tibial margin. Fracture size and morphology are important factors guide treatment these fragments, but reliability plain radiography in estimating is low. The aim current study was evaluate accuracy 2-dimensional computed tomography (2DCT) assessment malleolar fractures. Additionally, diagnostic 2DCT its value preoperative planning evaluated.
The role of growth factors (GFs) in controlling the biology human hematopoietic stem cells (HSCs) remains limited by a lack information concerning individual and combined effects GFs directly on survival, Mitogenesis, regenerative activity highly purified HSCs. We show that initial input HSC such starting population cord blood can be fully maintained over 21-day period serum-free medium containing five alone. survival was partially supported any one these GFs, but none were essential,...
10004 Background: Pediatric low-grade gliomas (LGGs) are the most common brain tumors of childhood. Genomic alterations BRAF ( KIAA1549-BRAF fusions, 50–60% and V600E mutations, 5–15%) frequent oncogenic drivers in pLGGs. Tovorafenib is an investigational, oral, selective, brain-penetrant, small molecule, type II pan-RAF inhibitor. has demonstrated clinically meaningful responses 24/35 patients (2 CR, 7 PR 15 SD) pediatric phase 1B PNOC014 (NCT03429803) trial with RAF-altered cancers...
ABSTRACT The maturation of the hepatitis C virus (HCV) core protein requires proteolytic processing by two host proteases: signal peptidase (SP) and intramembrane-cleaving protease peptide (SPP). Previous work on HCV genotype 1a (GT1a) GT2a has identified crucial residues required for efficient SPP, which in turn an effect production infectious particles. Here we demonstrate that JFH1 core-E1 can be adapted to GT3a sequence without affecting HCV. Through mutagenesis studies, processing,...
Background The Apple Watch is not a medical device per se; it smart wearable that increasingly being used for health monitoring. Evidence exists the Series 6 can reliably measure blood oxygen saturation (SpO2) in patients with chronic obstructive pulmonary disease under controlled circumstances. Objective This study aimed to better understand older adults’ acceptance of as part telemonitoring, even these advancements. Methods conducted content analysis on data collected from 10 adults who...
Abstract BACKGROUND RAF alterations are oncogenic drivers found in most pediatric low-grade gliomas (LGGs). Tovorafenib is an investigational, oral, selective, CNS-penetrant, small molecule, type II pan‑RAF inhibitor. METHODS FIREFLY-1 (NCT04775485) a multicenter phase 2 study evaluating the safety and efficacy of tovorafenib monotherapy. Registrational arm 1 enrolled patients with recurrent/progressive LGG harboring activating BRAF alteration. Patients aged 6 months–25 years who progressed...
Abstract BACKGROUND: Tovorafenib is an investigational, selective, CNS-penetrant, type II RAF inhibitor. The ongoing FIREFLY-1 (NCT04775485) phase 2 study (Kilburn LK, et al. Nat Med. 2023) of tovorafenib in BRAF-altered pLGG resulted antitumor activity and manageable safety. Decreased growth velocity (GV) was observed; this update on GV changes skeletally immature children receiving tovorafenib. Methods A planned safety analysis completed August 8, 2023 137 patients (Arm 1: 77 & Arm 2:...
Background Clinical practice settings have increasingly become dependent on the use of digital or eHealth technologies such as electronic health records. It is vitally important to support nurses in adapting digitalized care systems; however, little known about nursing graduates’ experiences they transition workplace. Objective This study aims (1) describe newly qualified nurses’ with workplace, and (2) identify strategies that could help new health. Methods An exploratory descriptive...
Summary Essential features of cancer tissue cellular heterogeneity such as negatively selected genome topologies, sub-clonal mutation patterns and replication states can only effectively be studied by sequencing single-cell genomes at scale high fidelity. Using an amplification-free approach implemented on commodity hardware (DLP+) coupled with a cloud-based computational platform, we define resource 40,000 characterized their states, across wide range types conditions. We show that shallow...
A new and improved synthetic route to an intermediate in the synthesis of phosphinate ester GSK2248761A is described. In key step, we describe first process-scale example a palladium-catalyzed phosphorus–carbon coupling give entire backbone one telescoped stage 65% average yield on 68 kg scale. This unusual chemistry enabled be reduced from six stages four eliminated number environmentally unfriendly reagents solvents.
Amlodipine is a calcium antagonist of the dihydropyridine class. In common with other agents in this class it can cause flushing and peripheral oedema.1 2 Erythema multiforme has been reported after amlodipine was substituted for nifedipine.3 We report two cases generalised pruritus secondary to treatment patients without objective evidence skin disease. A 59 year old woman diagnosed as being hypertensive 1994; …