A5059306952- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Identification and Quantification in Food
- Coral and Marine Ecosystems Studies
- Genetic and phenotypic traits in livestock
- Wildlife Ecology and Conservation
- Gut microbiota and health
- RNA modifications and cancer
- Marine and coastal plant biology
- Species Distribution and Climate Change
- Insect and Pesticide Research
- Molecular Biology Techniques and Applications
- Glioma Diagnosis and Treatment
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Wildlife Conservation and Criminology Analyses
- Echinoderm biology and ecology
- Plant Pathogens and Resistance
- Fish biology, ecology, and behavior
- 3D Modeling in Geospatial Applications
- ATP Synthase and ATPases Research
- Veterinary Medicine and Surgery
- Marine Ecology and Invasive Species
- Nanopore and Nanochannel Transport Studies
- Geographic Information Systems Studies
University of Nottingham
2023-2024
NERC Environmental Omics Facility
2024
Manchester Metropolitan University
2017-2022
University of Manchester
2013-2016
1. Plant-microbe interactions are critical for ecosystem functioning and drive rhizosphere processes. Root exudates an important soil carbon (C) input, as well a mechanism communication between plants microbes, but notoriously difficult to extract characterise. Common methods produce either substantial noise from the or do not mimic natural systems. Optimising root exudate collection in is crucial advancing our understanding of root-microbe under changing environmental conditions. 2. Hybrid...
Amphibian populations worldwide are at risk of extinction from infectious diseases, including chytridiomycosis caused by the fungal pathogen Batrachochytrium dendrobatidis (Bd). cutaneous microbiomes interact with Bd and can confer protective benefits to host. The composition microbiome itself is influenced many environment- host-related factors. However, little known about interacting effects host population structure, genetic variation developmental stage on prevalence across multiple...
Molecular data integration plays a central role in nervous system (CNS) tumor diagnostics but currently used assays pose limitations due to technical complexity, equipment and reagent costs, as well lengthy turnaround times. We previously reported the development of Rapid-CNS2, an adaptive-sampling-based nanopore sequencing workflow. Here we comprehensively validated further developed Rapid-CNS2 for intraoperative use. It now offers real-time methylation classification DNA copy number...
Microsatellites are useful tools for ecologists and conservationist biologists, but taxa-specific traditionally expensive time-consuming to develop. New methods using next-generation sequencing (NGS) have reduced these problems, the plethora of software available processing NGS data may cause confusion difficulty researchers new field bioinformatics. We developed a bioinformatics pipeline microsatellite development from Illumina paired-end sequences, which is packaged in open-source tool...
Insects preserved in copal, the sub-fossilized resin precursor of amber, have potential value molecular ecological studies recently-extinct species and extant that never been collected as living specimens. The objective work reported this paper was therefore to determine if ancient DNA is present insects copal. We prepared libraries from two stingless bees (Apidae: Meliponini: Trigonisca ameliae) 'Anthropocene' Colombian dated 'post-Bomb' 10,612±62 cal yr BP, respectively, obtained sequence...
Mangroves form coastal tropical forests in the intertidal zone and are an important component of shoreline protection. In comparison to other forests, mangrove stands thought have relatively low genetic diversity with population structure gradually increasing distance along a coastline. We conducted analyses across range spatial scales; within 400 m2 parcel comprising 181 Rhizophora mangle (red mangrove) trees, four sites ranging from 6-115 km apart Honduras. total, we successfully genotyped...
Abstract The 2021 WHO classification underscores the importance of molecular data integration in Central Nervous System (CNS) tumor diagnostics. However, currently used assays have disadvantages due to technical complexity, required equipment and reagent cost, as well lengthy turnaround times. In response these challenges, we introduce Rapid-CNS 2 MNP-Flex. , an adaptive sampling-based nanopore sequencing workflow, offers real-time methylation DNA copy-number information within a 30-minute...
Abstract Growth in the global development of cities, and increasing public interest beekeeping, has led to increase numbers urban apiaries. Towns cities can provide an excellent diet for managed bees, with a diverse range nectar pollen available throughout long flowering season, are often more ecologically than surrounding rural environments. Accessible honeybee hives valuable research resource gain insights into ecology wild pollinators settings. We used DNA metabarcoding rbcL ITS2 gene...
Bespoke microsatellite marker panels are increasingly affordable and tractable to researchers conservationists. The rate of discovery is very high within a shotgun genomic data set, but extensive laboratory testing markers required for confirmation amplification polymorphism. By incorporating next-generation sequencing sets from multiple individuals the same species, we have developed new method optimal design markers. This tool allows us increase at which suitable candidate selected by 58%...
Abstract Background Advances in our technological capacity to interrogate brain tumour biology has led the ever-increasing use of genomic sequencing routine diagnostic decision making. Presently, tumours are routinely classified based on their epigenetic signatures, leading a paradigm shift pathways. Such testing can be performed so rapidly using nanopore that results provided intraoperatively. This information greatly improves upon fidelity smear diagnosis and help surgeons tailor approach,...
ABSTRACT A species' demographic history gives important context to contemporary population genetics and a possible insight into past responses climate change; with an individual's genome providing window the evolutionary of populations. Pairwise sequentially Markovian coalescent (PSMC) analysis uses information from single derive fluctuations in effective size change over last ~5 million years. Here, we apply PSMC two European nightjar ( Caprimulgus europaeus ) genomes, sampled Northwest...
Amongst the global decline of coral reefs, hope spots such as Cordelia Bank in Honduras, have been identified. This site contains dense, remnant thickets endangered species Acropora cervicornis, which local managers and conservation organizations view a potential source population for restoration projects. The aim this study was to determine genetic diversity colonies across three banks within protected area. We identified low (FST = 0.02) banks, similarity ranged from 91.3 95.8% between...
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The majority of research focusing on coral reproductive biology (e.g. spawning timing and synchrony) is carried out in facilities adjacent to reefs that the corals originated from. This part because transporting over long distances by air leads sub-lethal stress may confound results any experimental study. However, these constraints often mean associated with restricted relatively few locations. To assess potential for studying environmental drivers captivity (defined here as ex-situ closed...
Abstract BACKGROUND The 2021 WHO classification update highlights the necessity of integrating molecular alterations for precise central nervous system (CNS) tumor diagnoses. However, current reporting methods are hindered by significant initial investment, labor-intensive protocols, and lengthy turnaround times. Methylation-based has emerged as a pivotal diagnostic tool but is currently limited to array-based techniques. This necessitates exploration novel technologies streamline analysis....
As a first step to establish the genetic structure of sea urchin Echinometra lucunter throughout Caribbean Sea, 26 microsatellite loci were isolated using Illumina paired-end sequencing, Next Generation Sequencing (NGS). We successfully optimized 17 for genotyping and variation tested 23 individuals from Sea Tropical Eastern Atlantic Ocean. The allele number per locus (Na) ranged four 24, observed heterozygosity (Ho) 0.682 1, expected (He) 0.609 0.9304. detected no linkage disequilibrium...
A species’ demographic history provides important context to contemporary population genetics and a possible insight into past responses climate change. An individual’s genome window the evolutionary of populations. Pairwise Sequentially Markovian Coalescent (PSMC) analysis uses information from single derive fluctuations in effective size change over last ~5 million years. Here we apply PSMC two European nightjar (Caprimulgus europaeus) genomes, sampled Northwest Southern Europe, with aim...