Both inhibitory and stimulatory (known as hormesis) effects of the sublethal flupyradifurone, a butenolide insecticide, on Myzus persicae Sulzer (Hemiptera: Aphididae) were investigated for incorporating it into integrated pest management (IPM). A leaf-dip bioassay showed that flupyradifurone was very toxic against adult M. with 48 h LC50 8.491 mg/L. Using age-stage two-sex life table approach, we assessed LC25 its progeny (F1 F2). On one hand, aphids exposed to had significantly negative...

Abstract It is desirable for a simple and universal method to synthesize stimuli‐responsive nanocarriers targeted delivery of pesticides. In this study, novel pH‐value laccase dual‐responsive nanopesticide system loaded with lambda‐cyhalothrin (LC) established by iron mineralization after electrostatic self‐assembly between sodium lignosulphonate (SL) dodecyl dimethyl benzyl ammonium chloride (DDBAC). These nanoparticles are core–shell structures in which plays key role stabilizing the...

Mg(OH)2 was used as the nanocarrier of Bacillus thuringiensis (Bt) Cry1Ac protein, and synthesized Cry1Ac–Mg(OH)2 composites were regular uniform nanosheets. Nano-Mg(OH)2 could effectively improve insecticidal effect protein toward Ectropis obliqua. It enhance damage degree to intestinal epithelial cells microvilli, induce enrich production reactive oxygen species (ROS) in midgut, degradation into active fragments. Furthermore, an anti-rinsing assay showed that bound notch structure tea leaf...

For invasive insects, the potential roles of gut microbiota in exploiting new food resources and spreading remain elusive. Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is an destructive pest which feeds on nutrient-poor tender tissues has caused extensive mortality trees. The microbes associated with insects can improve their nutrition assimilation. However, experimental evidence interactions between RPW its still absent. aim this study to determine dynamics changes bacterial...

The immune system of animals, including insects, is the vital factor to maintain symbiotic interactions between animals and their associated microbes. However, effects gut microbiota on insect immunity remain mostly elusive. Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, a destructive pest trees worldwide, which has forged alliances with its microbiota. Here, we found that aposymbiotic insects succumbed at significantly faster rate than conventionally reared (CR) ones upon...

Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is a destructive pest for trees worldwide. Recent studies have shown that RPW gut colonized by microbes and alterations in microbiota can significantly modify its hemolymph nutrition content. However, the exact effects of on phenotype underlying mechanisms remain elusive. Here germ-free (GF) larvae were generated from dechorionated eggs which reared sterilized artificial food under axenic conditions. Compared with controls, larval...

A strategy that relies on the differences in feeding behavior between pests and natural enemies to deliver insecticides precisely was proposed. After proving digestive enzymes Lepidopteran can act as triggers for lignin-based controlled-release carriers, a novel multiple-enzyme-responsive lignin/polysaccharide/Fe nanocarrier constructed by combining electrostatic self-assembly chelation loaded with lambda-cyhalothrin (LC) form nanocapsule suspension loading system. The nanocapsules were...

Bacterial symbionts of insects affect a wide array host traits including fitness and immunity. Octodonta nipae (Maulik), commonly known as hispid leaf beetle is destructive palm pest around the world. Understanding dynamics microbiota essential to unravel complex interplay between O. its bacterial symbionts. In this study, 16S rRNA V3-V4 region was targeted decipher diversity across different life stages [eggs, larvae, pupae, adult (male female)] reproductive organs (ovaries testis) nipae....

Tomato is more prone to Tuta absoluta invasion and damages as compared other host plants but the mechanism behind this preference has not been elucidated. Here, two contrasting plants, tomato eggplant, were used investigate biochemical transcriptomic modifications induced by T. infestation. Biochemical analysis at 0–72 h post infestation revealed significantly reduced concentrations of amino acid, fructose, sucrose, jasmonic salicylic total phenols in mainly 48 Transcriptome showed higher...

RNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases one of key factors affecting efficiency. In the current study, we identified four genes coding from Spodoptera frugiperda genome. Spatial and temporal expression analysis showed those were highly expressed in midgut old larvae. Then delivery method was applied inducing efficient based on dsRNA encapsulated by liposome. Furthermore, assessed...

Symbiotic systems are intimately integrated at multiple levels. Host–endosymbiont metabolic complementarity in amino acid biosynthesis is especially important for sap-feeding insects and their symbionts. In weevil–Nardonella endosymbiosis, the final step reaction of endosymbiont tyrosine synthesis pathway complemented by host-encoded aminotransferases. Based on previous results from other insects, we suspected that these aminotransferases were likely transported into Nardonella cytoplasm to...

RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of across diverse insects off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta conducted experiments by orally administering dsRNA. Then, found that mixing with liposomes significantly enhanced targeting for SiV-ATPaseE. Additionally, observed certain lethal effect dsRNA on queens our...

Insects have highly developed olfactory systems in which cytochrome P450s (CYPs) were involved as odor-degrading enzymes throughout the recognition of odor compounds by insects to avoid continuous stimulation signaling molecules and thus damage nervous. To understand whether expressed CYPs antennae play an function Solenopsis invicta worker, this study, we find six antennal from transcriptome S. invicta. Multiple sequence alignment phylogenetic analysis divided them into two families: CYP3...

Tuta absoluta is a significant agricultural pest primarily affecting Solanaceae plants, resulting in substantial economic losses agriculture. Insect saliva an intermediary between insects and playing crucial role modulating host adaptability plant defense. This study analyzed the adaptive differences of T. on four plants using two-sex life table method. Results indicated that to tobacco worse than its other three varieties tomatoes. The salivary gland transcriptome analysis signal peptide...

The tomato leafminer, Tuta absoluta, is an invasive pest that poses a significant threat to Solanaceae crops, including tomatoes, potatoes, and eggplants. Its ability adapt various environmental conditions, especially cold stress, allows it survive in regions with fluctuating temperatures. In this study, the molecular response of T. absoluta adults rapid hardening (RCH) under gradually decreasing non-lethal temperatures was investigated. Using RNA-seq, 197 upregulated genes 626 downregulated...

Pollen grains are regularly used as markers to determine an insect’s movement patterns or host (plant) feeding behavior, yet conventional morphology-based pollen grain analysis (or palynology) encounters a number of important limitations. In the present study, we combine analytical approaches with DNA meta-barcoding identify attached migrating adults turnip moth, Agrotis segetum (Lepidoptera: Noctuidae) in Northeast China. More specifically, were dislodged from 2566 A. long-distance migrants...

The beetle Octodonta nipae (Maulik) (Coleoptera: Chrysomelidae) is a serious invasive insect pest of palm plants in southern China, and the endoparasitoid Tetrastichus brontispae Ferrière (Hymenoptera: Eulophidae) natural enemy this that exhibits great ability biocontrol O. nipae. For successful parasitism, endoparasitoids often introduce or secrete various virulence factors to suppress host immunity. To investigate effects parasitization by T. on immune system, transcriptome pupae was...

Abstract BACKGROUND Rhynchophorus ferrugineus , the red palm weevil (RPW), is a key pest that attacks many economically important species and has evolved sensitive specific olfactory system to seek hosts. Odorant‐binding proteins (OBPs) not only play crucial roles in its perception process but are also molecular targets for development of new approaches management. RESULTS Analysis tissue expression profiles RferOBP8 RferOBP11 revealed these two odorant binding (RferOBPs) exhibited high...

The effect of temperature on the development, survivorship, fecundity, and life table parameters Octodonta nipae (Maulik) (Coleoptera: Chrysomelidae), was studied at seven constant temperatures 17.5, 20, 22.5, 25, 27.5, 30, 32.5°C. Preliminary experiments showed that no development observed 15 35°C. All individuals completed females laid eggs from 20 to 30°C. There a significant decrease in male female longevity with increasing longest shortest were 203.5 73.7 d for males, 178.7 57.6...