A5075601911- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Adipose Tissue and Metabolism
- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Metabolism, Diabetes, and Cancer
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- Biochemical Acid Research Studies
- Minerals Flotation and Separation Techniques
- Extraction and Separation Processes
- Lipid metabolism and biosynthesis
- Membrane Separation Technologies
- bioluminescence and chemiluminescence research
- Chemical and Physical Properties in Aqueous Solutions
- Biochemical and Molecular Research
- Machine Learning in Bioinformatics
- Peptidase Inhibition and Analysis
- Cardiovascular Function and Risk Factors
- Ubiquitin and proteasome pathways
- Steroid Chemistry and Biochemistry
Australian Regenerative Medicine Institute
2023-2025
Monash University
2023-2025
Wuhan University
2014-2024
Zhongnan Hospital of Wuhan University
2023-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Tianjin Medical University General Hospital
2024
University of Warwick
2023
ARC Centre of Excellence for Innovations in Peptide and Protein Science
2023
Nonribosomal peptides are chemically and functionally diverse natural products with important applications in medicine agriculture. Bacterial fungal genomes contain thousands of nonribosomal peptide biosynthetic gene clusters (BGCs) unknown function, providing a promising resource for discovery. Core structural features such can be inferred by predicting the substrate(s) adenylation (A) domains synthetases (NRPSs). However, existing approaches to A domain prediction rely on limited datasets...
Gentamicin C complex is a mixture of aminoglycoside antibiotics used worldwide to treat severe Gram-negative bacterial infections. Despite its clinical importance, the enzymology biosynthetic pathway has remained obscure. We report here insights into four enzyme-catalyzed steps that lead from first-formed pseudotrisaccharide gentamicin A2 X2, last common intermediate for all components complex. have both targeted mutations individual genes and reconstitution portions in vitro show secondary...
Gentamicin C complex is a mixture of aminoglycoside antibiotics used to treat severe Gram-negative bacterial infections. We report here key features the late-stage biosynthesis gentamicins. show that intermediate gentamicin X2, known substrate for C-methylation at C-6' form G418 catalyzed by radical SAM-dependent enzyme GenK, may instead undergo oxidation an aldehyde, flavin-linked dehydrogenase GenQ. Surprisingly, GenQ acts in both branches pathway, likewise oxidizing analogous ketone....
Aminoglycosides (AGs) are a class of antibiotics with broad spectrum activity. However, their use is limited by safety concerns associated nephrotoxicity and ototoxicity, as well drug resistance. To address these issues, semi-synthetic approaches for modifying natural AGs have generated new generations AGs, however, types modification due to significant challenges in synthesis. This study explores novel approach that harness the bacterial biosynthetic machinery gentamicins kanamycins create...
Abstract Aerobic training (AT), an effective form of cardiac rehabilitation, has been shown to be beneficial for repair and remodeling after myocardial infarction (MI). The p300/CBP‐associated factor (PCAF) is one the most important lysine acetyltransferases involved in various biological processes. However, role PCAF AT AT‐mediated post‐MI not determined. Here, we found that protein level was significantly increased MI, while blocked increase PCAF. markedly improved mice MI by reducing...
Abstract Advances in DNA sequencing technology and bioinformatics have revealed the enormous potential of microbes to produce structurally complex specialized metabolites with diverse uses medicine agriculture. However, these molecules typically require structural modification optimize them for application, which can be difficult using synthetic chemistry. Bioengineering offers a complementary approach but is often hampered by genetic intractability requires thorough understanding...
Gentamicin is an important aminoglycoside antibiotic used for treatment of infections caused by Gram-negative bacteria. Although most the biosynthetic pathways gentamicin have been elucidated, a remaining intriguing question how intermediates JI-20A and JI-20B undergo dideoxygenation to form C complex. Here we show that process starts with GenP-catalyzed phosphorylation JI-20Ba. The phosphorylated products are successively modified concerted actions two PLP (pyridoxal 5′-phosphate)-dependent...
Abstract Gentamicin is an important aminoglycoside antibiotic used for treatment of infections caused by Gram-negative bacteria. Although most the biosynthetic pathway gentamicin has been elucidated, a remaining intriguing question how intermediates JI-20A and JI-20B undergo dideoxygenation to form C complex. Here we show that process starts with GenP-catalyzed phosphorylation JI-20Ba. The phosphorylated products are converted C1a C2a concerted actions two PLP (pyridoxal...
Abstract Aminoglycosides (AGs) are a class of potent antibiotics with broad spectrum activity. However, their use is limited by safety concerns associated nephrotoxicity and ototoxicity, as well drug resistance. To address these issues, semi-synthetic approaches for modifying natural AGs have successfully generated new generations AGs, however, types modification due to significant challenges in synthesis. This study explores novel approach that harness the bacterial biosynthetic machinery...
Abstract Gentamicin is an important aminoglycoside antibiotic used for treatment of infections caused by Gram-negative bacteria. Although most the biosynthetic pathway gentamicin has been elucidated, a remaining intriguing question how intermediates JI-20A and JI-20B undergo dideoxygenation to form C complex. Here we show that process starts with GenP-catalyzed phosphorylation JI-20Ba. The phosphorylated products are converted C1a C2a concerted actions two PLP (pyridoxal...