A5081161777- Dermatology and Skin Diseases
- Diet and metabolism studies
- Cholesterol and Lipid Metabolism
- Cancer-related molecular mechanisms research
- Diabetes and associated disorders
- Endoplasmic Reticulum Stress and Disease
- Cancer, Lipids, and Metabolism
- Lipoproteins and Cardiovascular Health
- RNA modifications and cancer
- Acne and Rosacea Treatments and Effects
- Immune cells in cancer
- Advancements in Transdermal Drug Delivery
- Lipid metabolism and disorders
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Hormonal Regulation and Hypertension
- Enzyme Structure and Function
- Diverse Music Education Insights
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Acute Ischemic Stroke Management
- Heat shock proteins research
- Intracerebral and Subarachnoid Hemorrhage Research
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
Beijing Technology and Business University
2018-2024
Chongqing Cancer Hospital
2020-2024
Chongqing University
2020-2024
Nanjing Agricultural University
2018-2024
University of California, San Francisco
2019-2024
Pudong Medical Center
2023
Fudan University
2023
Yueyang Changling Equipment Research Institute (China)
2023
Capital Normal University
2023
Beijing Sanbo Brain Hospital
2020-2022
Abstract Cancer development and its response to therapy are regulated by inflammation, which either promotes or suppresses tumor progression, potentially displaying opposing effects on therapeutic outcomes. Chronic inflammation facilitates progression treatment resistance, whereas induction of acute inflammatory reactions often stimulates the maturation dendritic cells (DCs) antigen presentation, leading anti-tumor immune responses. In addition, multiple signaling pathways, such as nuclear...
Apolipoprotein B (apoB) is the major protein component of atherogenic lipoproteins hepatic origin. In HepG2 cells, standard cell culture model human lipoprotein metabolism, there a limited availability core lipids in endoplasmic reticulum for association with nascent apoB. Under these conditions, apoB partially translocated, interacts cytosolic Hsp70, and undergoes rapid degradation. We show that increasing expression Hsp70 cells promotes addition, polyubiquitinated its degradation both...
Abstract We examined the relation between insulin resistance, plasma glucose and responses to meals, lipoprotein lipase (LPL) activity, postprandial lipemia in a population of 37 healthy nondiabetic individuals. Plasma concentrations were determined at frequent intervals from 8 am through midnight (breakfast lunch noon); resistance insulin-mediated disposal was by measuring steady-state (SSPG) concentration end 180-minute infusion glucose, insulin, somatostatin; LPL activity quantified...
Objective: This study was aimed to examine the psychological impact of COVID-2019 outbreak on medical staff in China.Methods: In February 2020, an online survey carried out by using a WeChat tool evaluate effects mental health around China.Results: A total 11118 responded survey. About 4·98% respondents reported middle and high levels anxiety while 13·47% depression since COVID-19 outbreak. Nurses, frontline younger were more likely have than physicians, non- older respectively.Conclusions:...
Details of intestinal sterol absorption and inhibition by cholesterol-lowering drug are uncovered in new protein structures.
Presentation of a wild-type secretory protein, apolipoprotein B100 (apoB), to the cytosol for ubiquitin-proteasome proteolysis has been observed in HepG2 cells. A currently accepted model proteasomal degradation proteins is retrograde translocation substrate polypeptides from lumen endoplasmic reticulum (ER) back cytosol. In this report, we present evidence that newly synthesized apoB becomes exposed and targeted proteasomes co-translational manner. Thus, after protein translation was...
Microsomal triglyceride (TG) transfer protein (MTP) is an endoplasmic reticulum lumenal consisting of a 97-kDa subunit and disulfide isomerase. It believed that MTP delivers TG to nascent apoB molecules during the assembly lipoprotein particles in secretory pathway. Although vitro studies have established mechanism between donor acceptor membranes, action vivo remains unknown. The present were undertaken examine whether or not involves physical interaction apoB. HepG2 cells labeled with...
OBJECTIVE To understand why low-fat high-carbohydrate (CHO) diets lead to higher fasting and postprandial concentrations of triglyceride (TG)-rich lipoproteins in patients with non-insulin-dependent diabetes mellitus (NIDDM). RESEARCH DESIGN AND METHODS Patients NIDDM were placed randomly on containing either 55% CHO, 30% fat, 15% protein or 40% 45% for 6 weeks, followed by crossover the other diet. Test meals at end each diet period consumed 8:00 A.M. 12:00 P.M. (noon) contained 20 daily...
To quantify the effect of metformin on metabolism triglyceride (TG)-rich lipoprotein intestinal origin in patients with non-insulin-dependent diabetes mellitus (NIDDM) who had responded to sulfonylurea but still fasting hyperglycemia.Sixteen NIDDM demonstrated a fall plasma glucose concentration > 2.2 mmol/l response glipizide treatment continued have concentrations 8.3 were studied. Fasting glucose, GHb, lipid and determined, resistance insulin-mediated disposal was estimated by measuring...
Acne is a complex and multifactorial skin disorder. Alterations in surface lipid (SSL) are believed to be an important factor the pathogenesis of acne SSL plays key role initiation lesions. To analyse lipidome profiles patients with healthy controls order understand abnormity young men. Ultraperformance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry (UPLC‐QTOF‐MS) multivariate data analysis were used investigate variations main classes, subclasses individual species....
Small nucleolar RNA host gene (SNHG) long noncoding RNAs (lncRNAs) are frequently dysregulated in human cancers and involved tumorigenesis progression. SNHG17 has been reported as a candidate oncogene several cancer types, however, its regulatory role colorectal (CRC) is unclear.SNHG17 expression multiple CRC cohorts was assessed by RT-qPCR or bioinformatic analyses. Cell viability evaluated using Counting Kit-8 (CCK-8) colony formation assays. mobility invasiveness were Transwell Tumor...
When lipid synthesis is limited in HepG2 cells, apoprotein B100 (apoB100) not secreted but rapidly degraded by the ubiquitin-proteasome pathway. To investigate apoB100 biosynthesis and secretion further, physical functional states of destined for either degradation or lipoprotein assembly were studied under conditions which synthesis, proteasomal activity, microsomal triglyceride transfer protein (MTP) lipid-transfer activity varied. Cells pretreated with a inhibitor (which remained cells...
Elevated triglyceride (TG) and cholesterol levels are risk factors for cardiovascular disease often associated with diabetes metabolic syndrome. Recent reports suggest that fibroblast growth factor (FGF)19 FGF21 can dramatically improve dysfunction, including hyperglycemia, hypertriglyceridemia, hypercholesterolemia. Due to their similar receptor specificities co-receptor requirements, FGF19 share many common properties have been thought be interchangeable in regulation. Here we directly...
Objective: To examine the effects of comprehensive nursing interventions on maternal and infant outcomes in pregnant women diagnosed with gestational diabetes mellitus (GDM). Methods: A quasi-experimental design was employed, involving 60 GDM who were purposively selected randomly allocated into experimental control groups, each comprising 30 participants. The group received pregnancy monitoring, while standard care. Data collection conducted using demographic questionnaires, indicators,...
Newly synthesized apolipoprotein B (apoB) undergoes rapid degradation in a pre-Golgi compartment HepG2 cells. A major site of this early seems to be on the cytosolic side endoplasmic reticulum (ER) membrane and is sensitive N-acetyl-leucinyl-leucinyl-norleucinal (ALLN), which can inhibit neutral cysteine proteases and/or proteasome activity. Oleate (OA) treatment, facilitates translocation nascent apoB across ER membrane, also reduces degradation. In present studies, we have used brefeldin...
LCAT is intimately involved in HDL maturation and a key component of the reverse cholesterol transport (RCT) pathway which removes excess molecules from peripheral tissues to liver for excretion. Patients with loss-of-function mutations exhibit low levels corneal opacity. Here we report 2.65 Å crystal structure human protein. Crystallization required enzymatic removal N-linked glycans complex formation Fab fragment tool antibody. The reveals that has an α/β hydrolase core two additional...
Cardiovascular disease is expected to remain the leading cause of death worldwide despite introduction proprotein convertase subtilisin/kexin type 9 inhibitors that effectively control cholesterol. Identifying residual risk factors for cardiovascular remains an important step preventing and clinically managing disease. Here we report cardiac injury increased mortality occurring a 50% reduction in plasma cholesterol mouse model phytosterolemia, characterized by elevated levels dietary plant...
Abstract Background Acne is a multifactorial skin disorder frequently observed during adolescence with different grades of severity. The crucial factors acne are the increase lipids secretion and change composition on surface lipid (SSL). However, there no studies changes content between adolescent in lesional nonlesionsal skin. Aims This study was to investigate correlation SSL order understand tendency alterations this disease for successful management prevention. Methods A powerful...
Abstract Background Accumulating evidences have shown that long noncoding RNAs (lncRNAs) play key roles in many diseases, including cancer. Several studies reported MCM3AP antisense RNA 1 (MCM3AP‐AS1) was associated with the tumorigenesis and progression. However, specific function mechanism of MCM3AP‐AS1 colorectal cancer (CRC) not been fully understood. Methods The expression detected by quantitative reverse transcription PCR (RT‐qPCR) CRC tissues matched noncancerous (NCTs). CCK‐8 assay,...
Heat shock protein 72/73 (Hsp70) is a cytosolic molecular chaperone that carries out fundamental roles under both normal and stress situations. There great interest in delineating the mechanisms whereby Hsp70 levels are regulated. We observed N-acetyl-leucyl-leucyl-norleucinal (ALLN), synthetic aldehydic tripeptide inhibits proteasomes, markedly induced (up to 30-fold above base line HepG2 cells human endothelial cells). Induction of by ALLN was dose-dependent not related cell toxicity....
Apoprotein B100 (apoB) is a secretory protein that appears to be constitutively translated but inefficiently translocated into the lumen of endoplasmic reticulum. Using several experimental approaches, we found apoB bound cytosolic chaperone protein, heat shock 72/73 (commonly referred as Hsp70). Similar other chaperone-protein interactions, this binding was transient and ATP-sensitive. The Hsp70 in HepG2 cells decreased by treatment with oleic acid, which increases both translocation...