A5100457655- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- High Altitude and Hypoxia
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Advanced Radiotherapy Techniques
- Adipose Tissue and Metabolism
- Advanced biosensing and bioanalysis techniques
- Microbial Inactivation Methods
- RNA Interference and Gene Delivery
- ATP Synthase and ATPases Research
- IL-33, ST2, and ILC Pathways
- Cytokine Signaling Pathways and Interactions
- Fermentation and Sensory Analysis
- Phytochemicals and Antioxidant Activities
- Mesenchymal stem cell research
- Pregnancy and preeclampsia studies
- Effects of Radiation Exposure
- Nanoparticles: synthesis and applications
- Enzyme Catalysis and Immobilization
- Neurogenesis and neuroplasticity mechanisms
- Cancer Mechanisms and Therapy
- Ubiquitin and proteasome pathways
Shandong Academy of Sciences
2024
Qilu University of Technology
2024
Institute of Agricultural and Food Biotechnology
2023
Affiliated Hospital of Hangzhou Normal University
2022
Shizuoka General Hospital
2022
Wenzhou Medical University
2021
First Affiliated Hospital of Wenzhou Medical University
2021
First People's Hospital of Jingzhou
2020
Xian Yang Central Hospital
2020
South China Normal University
2018
MicroRNAs are important gene regulators that potentially play a profound role in tumorigenesis. Increasing evidence indicates miR-145 is tumor suppressor capable of inhibiting breast and colon cancer cell growth both vitro vivo. However, the biological function non-small lung (NSCLC) largely unknown. In cells, c-Myc confirmed direct target for miR-145. The aim this work was to investigate effect on proliferation NSCLC using lines A549 H23 as models. We determined expression level tissues...
In the current work, influence of high-pressure homogenization (HPH) (200, 250, and 300 MPa) on pH, Brix, turbidity, viscosity, particle size distribution (PSD), zeta potential, color, polyphenol oxidase (PPO), peroxidase (POD), profile bioaccessibility total phenolic compounds was studied. The results show no change in apple juice’s TSS density. contrast, other physiochemical properties juice treated with HPH were significantly changed. Besides content (15% degradation) HPH-treated at MPa,...
MicroRNA‑19a (miR‑19a) has been found to be overexpressed in lung cancers. However, the underlying molecular mechanisms of miR‑19a tumorigenesis and development cancer remain poorly understood. In present study, we aimed delineate role action non‑small cell (NSCLC). was both NSCLC tumor tissues lines, as shown by RT-PCR. The enforced expression transfection with miR-19a mimics significantly enhanced growth viability, invasion migration cells, assays, promoted xenograft tumors a mouse model....
Allergic rhinitis (AR) is primarily caused by a T helper cell (Th)1/Th2 imbalance. In murine AR model of previous study, the serum ovalbumin (OVA)-sIgE concentration was high, whereas microRNA (miR)-135a lowly expressed in nasal mucosa. The abnormal expression pattern miR-135a coincided with highly endogenous factors, including GATA binding protein (GATA)-3 and interleukin (IL)-4, T-box cells (T-bet) interferon (IFN)-γ. We hypothesized that may play an important role immune regulation mice....
In muscular dystrophy and old age, skeletal muscle repair is compromised leading to fibrosis fatty tissue accumulation. Therefore, therapies that protect or enhance would be valuable medical treatments. Hypoxia-inducible factors (HIFs) regulate gene transcription under conditions of low oxygen, HIF target genes EPO VEGF have been associated with protection repair. We tested the importance activation following injury, in both a murine model human volunteers, using prolyl hydroxylase...
The objective of this study was to explore the role SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression detected tumor tissues and tumor-adjacent normal from cancer patients. HeLa cells were divided into six groups: control group, NC siRNA + LY294002 U0125 group. In each received corresponding treatment, followed by measurement cellular biological characteristics pathway. We also confirmed effect vivo using a xenograft model nude mice. upregulated tissues, which...
Multidrug resistance (MDR) is the primary barrier to success of chemotherapy for lung adenocarcinoma. MicroRNA (miR)-134, which downregulated in adenocarcinoma, influences cell proliferation, apoptosis and invasion However, function miR-134 MDR adenocarcinoma remains unclear. In present study, it was identified that expression significantly A549/cisplatin cells, as compared with A549 parental cells. regulates sensitivity cells certain anticancer drugs. Furthermore, demonstrated forkhead box...
MicroRNA (miR)‑199a‑5p expression is downregulated in a variety of malignancies, including non‑small cell lung cancer (NSCLC), and its low associated with poor prognosis. However, to the best our knowledge, mechanism underlying miR‑199a‑5p downregulation NSCLC target effectors remain be elucidated. The present study revealed tissues lines compared para‑carcinoma epithelial line. Further experiments indicated that methylation levels miR‑199a promoter were markedly higher tissues. DNA...
Identify the controversial role of miRNA-34a as an oncogene or tumor suppressor. Simultaneous fluorescence imaging and caspase-3 in living cells.
Background: Ischemic spinal cord injury (ISCI) results in the motor sensory dysfunction of limbs below site. In response to injury, astrocytes develop into neuroprotective (A2s) mitigate damage.
旨在分析鹅MyoG基因启动子活性区域和转录因子，探究该基因的转录调控机制。本研究首先通过PCR扩增泰州鹅MyoG基因5'侧翼区序列1 245 bp并对其进行测序和生物信息学分析，其次，构建4个不同缺失片段的双荧光素酶报告载体，转染C2C12细胞系。进一步利用在线软件预测核心启动子区关键转录因子，对转录因子结合位点HNF4(-521~-503 bp)、USF (-379~-370 bp)和E2(-296~-281 bp)进行定点突变并构建突变报告基因载体，在C2C12细胞系内初步鉴定MyoG基因核心转录调控因子。最后，采集70日龄泰州鹅胸肌、腿肌、心、肝、脾、肺、肾和下丘脑组织样，利用荧光定量PCR检测MyoG基因和核心转录调控因子的组织表达谱。结果表明，扩增得到的鹅MyoG基因5'侧翼区序列包含启动子元件；利用双荧光素酶报告载体检测到鹅MyoG基因启动子区-624~-154 bp区域存在关键顺式调控元件；结合定点突变技术初步鉴定USF是鹅MyoG基因核心转录调控元件。组织表达谱研究进一步表明，MyoG和USF基因在鹅8个不同组织中均有表达，且在胸肌、腿肌和心组织中共同高表达(P...