Verticillium wilt is a soil-borne disease, and severely limits the development of cotton production. To investigate role endophytic fungi on wilt, CEF-818 (Penicillium simplicissimum), CEF-714 (Leptosphaeria sp.), CEF-642 (Talaromyces flavus.) CEF-193 (Acremonium sp.) isolated from roots were used to assess their effects against disease caused by defoliating V. dahliae strain Vd080. In greenhouse, all treatments significantly reduced incidence index, with control efficacy ranging 26%...

As a result of changing consumer preferences, cotton (Gossypium Hirsutum L.) from varieties with naturally colored fibers is becoming increasingly sought after in the textile industry. The molecular mechanisms leading to fiber development are still largely unknown, although it expected that color derived flavanoids.Firstly, four key genes flavonoid biosynthetic pathway (GhC4H, GhCHS, GhF3'H, and GhF3'5'H) were cloned studied their expression profiles during brown- white by QRT-PCR. And then,...

Verticillium wilt caused by dahliae is a common soil-borne disease worldwide, affecting many economically important crop species. Soil microbes can influence plant development. We investigated rhizosphere and endosphere microbiomes in relation to cotton cultivars with differential susceptibility wilt. samples from nine were assessed for the density of V. microsclerotia; plants used amplicon sequencing profile both bacterial fungal communities. Unlike severity, inoculum did not differ...

Abstract Background The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses has been studied in plants, however, limited is known about the 4CL genes cotton ( G. hirsutum L.) and their roles response to drought stress. Results We performed genome-wide identification investigated expression profiles identified various tissues stress conditions with an aim identify gene(s) associated tolerance. 34 putative that were clustered into three classes. Genes same class usually share a...

Rhizosphere microbial communities are known to be related plant health; using such an association for crop management requires a better understanding of this relationship. We investigated rhizosphere microbiomes associated with Verticillium wilt symptoms in two cotton cultivars. Microbial were profiled by amplicon sequencing, the total bacterial and fungal DNA quantified quantitative polymerase chain reaction based on respective 16S internal transcribed spacer primers. Although level V....

Verticillium wilt is a kind of plant vascular disease caused by the soilborne fungus dahliae, which severely limits cotton production. Our previous studies showed that endophytic Gibellulopsis nigrescens CEF08111 can effectively control and induce defense response in plants. However, comprehensive molecular mechanism governing this not yet clear. To study signaling induced strain CEF08111, transcriptome seedlings pretreated with was sequenced. The results revealed 249, 3559 33 differentially...

The structural characteristics and component differences of proanthocyanidins in brown white cotton fibres were identified by nuclear magnetic resonance (NMR) matrix-assisted laser desorption/ionization time flight mass spectrometry (MALDI-TOF MS) analyses. Proanthocyanidins found to contain mainly procyanidin (PC) prodelphidin (PD) units with 2, 3- cis form (epigallocatechin epicatechin). However, part the modified acylation constitutively different from fibres. relative amount PD was...

Cotton Verticillium wilt, caused by the notorious fungal phytopathogen dahliae (V. dahliae), is a destructive soil-borne vascular disease and severely decreases cotton yield quality worldwide. Transcriptional post-transcriptional regulation of genes responsive to V. are crucial for tolerance in plants. However, specific microRNAs (miRNAs) miRNA/target gene crosstalk involved resistance wilt remain largely limited. To investigate roles regulatory RNAs under induction upland cotton, mRNA small...

Rhizosphere microbial communities are recognized as crucial products of intimate interactions between plant and soil, playing important roles in growth health. Enhancing the understanding this process is a promising way to promote next green revolution by applying multifunctional benefits coming with rhizosphere microbiomes. In study, we propagated eight cotton genotypes (four upland cultivars four sea-land cultivars) varying levels resistance Verticillium dahliae three distinct soil types....

Glycoside hydrolase (GH) family members act as virulence factors and regulate plant immune responses during pathogen infection. Here, we characterized the GH28 member endopolygalacturonase VdEPG1 in Verticillium dahliae. acts a factor V. dahliae The expression level of was greatly increased inoculated on cotton roots. suppressed VdNLP1-mediated cell death by modulating pathogenesis-related genes Nicotiana benthamiana. Knocking out led to significant decrease pathogenicity cotton. deletion...

Protein lysine acetylation is an important post-translational modification mechanism involved in cellular regulation eukaryotes. Calmodulin (CaM) a ubiquitous Ca2+ sensor eukaryotes and crucial for plant immunity, but it so far unclear whether CaM-mediated immunity. Here, we found that GhCaM7 acetylated upon Verticillium dahliae (V. dahliae) infection positive regulator of V. resistance. Overexpressing cotton Arabidopsis enhances resistance knocking-down makes more susceptible to dahliae....

Endophytic fungi live in healthy plant tissues and organs are a major source of natural bioactive compounds. In this study, we found that an endophytic fungus, Talaromyces purpureogenus CEF642N, isolated from the cotton roots, suppressed Verticillium dahliae by up to 53% after 15 days confrontation culture. Genome sequencing CEF642N mass spectrometry study its metabolites were used identify primary antagonists. To further elucidate antagonistic mechanism, transcriptome analysis...

Verticillium wilt of cotton, caused by the soilborne pathogen dahliae , is one most serious diseases cotton worldwide. Increased concerns about side effects synthetic pesticides have resulted in greater interest developing biocontrol strategies against wilt. We evaluated a Fusarium solani CEF559 isolate, obtained from endosphere healthy plants, for its potential V . vitro and vivo In addition to disease assessment, three key genes lignin metabolism pathway four pathogenesis-related (PR) were...

Verticillium wilt is a plant vascular disease caused by the soilborne fungus dahliae that severely limits cotton production. In previous study, we screened Bacillus cereus YUPP-10, an efficient antagonistic bacterium, to uncover mechanisms for controlling verticillium wilt. Here, report novel antimicrobial cyclodextrin glycosyltransferase (CGTase) from YUPP-10. Compared other CGTases, six different conserved domains were identified, and mutants constructed gene splicing with overlap...

Verticillium wilt is threatening the world’s cotton production. The pathogenic fungus dahliae can survive in soil form of microsclerotia for a long time, colonize through root cotton, and invade into vascular bundles, causing yellowing wilting leaves, serious cases, leading to plant death. Breeding resistant varieties most economical effective method control wilt. In previous studies, proteomic analysis was carried out on different inoculated with V. strain Vd080. It found that GhRPS6...

The 12-oxo-phytodienoic acid reductases (OPRs) have been proven to play a major role in plant development and growth. Although the classification functions of OPRs well understood Arabidopsis, tomato, rice, maize, wheat, information OPR genes cotton genome their responses biotic abiotic stresses not reported. In this study, we found 10 9 Gossypium hirsutum barbadense, respectively. They were classified into three groups, based on similar gene structure conserved protein motifs. These just...

Verticillium dahliae is a soil-borne fungus that causes vascular wilt through the roots of plants. caused by V. one main diseases in cotton producing areas world, resulting huge economic losses. Breeding resistant varieties most economical and effective method to control wilt. Calcium-dependent protein kinases (CDPKs) play pivotal role plant innate immunity, including regulation oxidative burst, gene expression as well hormone signal transduction. However, function CDPKs response stress...

Verticillium wilt, mainly caused by a soil-inhabiting fungus dahliae, can seriously reduce the yield and quality of cotton. The complex mechanism underlying cotton resistance to wilt remains largely unknown. In plants, reactive oxygen species (ROS) mediated Rbohs is one earliest responses plants biotic abiotic stresses. our previous study, we performed time-course phospho-proteomic analysis roots resistant susceptible varieties in response V. found early differentially expressed protein...

Verticillium dahliae could cause destructive vascular wilt disease on hundreds of plant species around the world, including cotton. In this study, we characterized function a hydrophobin gene VdHP1 in pathogen development and pathogenicity. Results showed that induce cell death activate immune responses. The deletion mutants (ΔVdHP1) complement (C-ΔVdHP1) were obtained by homologous recombination method. exhibited increased hydrophilicity, inhibited microsclerotial formation, reduced spore...