A5069962006- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Pharmacogenetics and Drug Metabolism
- Skin and Cellular Biology Research
- Clinical Laboratory Practices and Quality Control
- Psychotherapy Techniques and Applications
- Psychological Testing and Assessment
- Protein Kinase Regulation and GTPase Signaling
- Hemodynamic Monitoring and Therapy
- Pharmacological Effects and Toxicity Studies
- Autoimmune Bullous Skin Diseases
- Personality Disorders and Psychopathology
- Cellular Mechanics and Interactions
- CRISPR and Genetic Engineering
- Methemoglobinemia and Tumor Lysis Syndrome
- Treatment of Major Depression
- Melanoma and MAPK Pathways
- Genomics and Chromatin Dynamics
- HIV/AIDS drug development and treatment
- Neonatal Health and Biochemistry
- Geology and Paleoclimatology Research
- Hemoglobinopathies and Related Disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Systemic Sclerosis and Related Diseases
- Plant Gene Expression Analysis
Leiden University Medical Center
2012-2025
Performance Communications
2017
Lunar and Planetary Institute
2015
Erasmus MC
2003-2009
Erasmus University Rotterdam
2004-2009
Boston Children's Hospital
2007
Rotterdam University of Applied Sciences
2001-2002
Leiden University
1990-2001
Aims To characterize the demographic and pharmacogenetic factors that influence interpatient variability in plasma concentrations of HIV non‐nucleoside reverse transcriptase inhibitor efavirenz. Methods Data from all samples analyzed for efavirenz our TDM service 2002 2003 were reviewed. Information on gender, age, body weight, height, race, hormonal contraceptive use (in a subset patients), time between sampling last intake was recorded. PCR‐restriction fragment length polymorphism analysis...
Abstract Purpose: The purpose is to identify the demographic, physiologic, and inheritable factors that influence CYP3A activity in cancer patients Experimental Design: A total of 134 (62 females; age range, 26 83 years) underwent erythromycin breath test as a phenotyping probe CYP3A. Genomic DNA was screened for six variants suspected functional relevance CYP3A4 (CYP3A4*1B, CYP3A4*6, CYP3A4*17, CYP3A4*18) CYP3A5 (CYP3A5*3C CYP3A5*6). Results: (AUC0–40min) varied up 14-fold this population....
The RAD3 gene of Saccharomyces cerevisiae encodes an ATP-dependent 5′–3′ DNA helicase, which is involved in excision repair ultraviolet radiation damage. By hybridisation a Schizosaccharomyces pombe genomic library with probe we have isolated the S.pombe homologue . We also cloned radl5 by complementation radiation-sensitive phenotype mutant. Comparison restriction map and sequence, shows that identical to homologous S.cerevlslae , identified hybridisation. S.pomberadl5.P mutant highly...
After UV irradiation, the transcriptionally active MAT alpha locus in Saccharomyces cerevisiae is preferentially repaired compared with inactive HML locus. The effect of rad mutations from three different epistasis groups on differential repair was investigated. Three mutants, rad9, rad16, and rad24, were impaired removal dimers locus, whereas they had generally normal Since RAD9 necessary for G2 arrest after we propose that stage plays a role making accessible repair, at least repressed
Abstract Purpose: To evaluate the effect of naturally occurring variants in genes encoding cytochrome P450 (CYP) isoforms CYP3A4 and CYP3A5 patients with cancer receiving midazolam as a phenotyping probe. Experimental Design: Five were evaluated 58 (21 women 37 men) short i.v. bolus (dose, 0.0145 or 0.025 mg/kg). Midazolam concentrations plasma determined using liquid chromatography-mass spectrometry, pharmacokinetic variables calculated noncompartmental analysis. Genomic DNA was...
Abstract Building on results reported in Sellbom, Graham, and Schenk (2005), this study, we examined the incremental validity of newly introduced MMPI–2 (Butcher et al., 2001) Restructured Clinical (RC) scales (Tellegen 2003) over both Content scales. Participants were 647 clients private practice who administered Multiaxial Diagnostic Inventory (Doverspike, 1990) early therapy. The indicate that RC had acceptable internal consistency, reduced intercorrelations (compared to scales),...
Human immunodeficiency virus (HIV)-infected individuals show large interindividual variation in response to antiretroviral therapy. Efavirenz (EFV) and nevirapine (NVP) are nonnucleoside reverse transcriptase inhibitors, which prescribed combination with other therapy so-called highly active Recent studies provide evidence for the role of cytochrome P450 (CYP) genes, particular CYP2B6, relation EFV NVP pharmacokinetics. In this study, authors investigated whether common ABCB1, CYP2A6,...
Male germ cell tumors (GCTs) are extremely sensitive to platinum-containing chemotherapy, with only 10% of patients showing therapy resistance. However, the biological basis high curability disseminated GCTs by chemotherapy is still unknown. Recently, we demonstrated that mammalian serine/arginine rich protein-specific kinase 1 (SRPK1) a cisplatinsensitive gene, inactivation which leads cisplatin Because, in mammalians, expression SRPK1 preferentially testicular tissues, responsiveness male...
The therapeutic potential of antitumor drugs is seriously limited by the manifestation cellular drug resistance. We used budding yeast <i>Saccharomyces cerevisiae</i> as a model system to identify novel mechanisms resistance one most active anticancer agents, cisplatin. pinpointed <i>NPR2</i> (nitrogen permease regulator 2) gene whose disruption conferred In addition, we observed 4-fold cross-resistance <i>npr2</i>Δ cells (i.e., from which had been disrupted) doxorubicin, in combination with...
The Minnesota Multiphasic Personality Inventory-2 (MMPI-2) Psychopathology-Five (PSY-5) scales were developed to measure abnormal personality symptomatology. present study examines the incremental validity of PSY-5 beyond clinical and content in assessing criteria associated with disorders. current sample includes 240 male 407 female clients from private practice settings who completed MMPI-2 Multiaxial Diagnostic Inventory (MDI), a self-report checklist Statistical Manual Mental Disorders...
The StatSensor® Xpress-i™, a point-of-care system for blood creatinine measurement, offers patients the possibility of self-monitoring creatinine. In this study, analytical performance both detecting current renal function and monitoring (dys)function in kidney transplant was examined.Accuracy with capillary venous whole evaluated compared to an isotopic dilution mass spectrometry (IDMS)-traceable enzymatic test serum (n=138). Twenty Li-heparin samples were IDMS reference method performed by...
Moving average quality control (MA QC) is a patient-based real-time system. Advantages compared to conventional periodic internal (IQC) include absence of commutability problems and continuous monitoring performance. We implemented MA QC for multiple routine hematology chemistry parameters. describe the evaluation process provide practical tools aid implementation.Nine parameters (serum sodium, calcium, bicarbonate free thyroxine, hemoglobin [Hb], mean corpuscular volume, concentration...
Abstract Clin Chem Lab Med 2007;45:1536–41.
The therapeutic potential of the highly active anticancer agent cisplatin is severely limited by occurrence cellular resistance. A better understanding molecular pathways involved in cisplatin-induced cell death could potentially indicate ways to overcome unresponsiveness drug and thus lead treatment results. We used budding yeast <i>Saccharomyces cerevisiae</i> as a model organism identify characterize novel genes kill, found that<i>SKY1</i> (SR-protein-specific kinase from yeast)...