A5035855782- Trypanosoma species research and implications
- Antibiotic Resistance in Bacteria
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Medical Device Sterilization and Disinfection
- Gut microbiota and health
- Hepatitis B Virus Studies
- Lipoproteins and Cardiovascular Health
- Aquaculture disease management and microbiota
- Cervical Cancer and HPV Research
- Antibiotic Use and Resistance
- Hepatitis Viruses Studies and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Central Venous Catheters and Hemodialysis
- Natural product bioactivities and synthesis
- Molecular Biology Techniques and Applications
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Enterobacteriaceae and Cronobacter Research
- Nosocomial Infections in ICU
- Salmonella and Campylobacter epidemiology
Universidade Federal de Santa Catarina
2015-2025
National Council for Scientific and Technological Development
2022
Instituto de Ciências Farmacêuticas
2012
A One Health approach for antimicrobial resistance must integrate whole-genome sequencing surveillance data of critical priority pathogens from human, animal and environmental sources to track hot spots routes transmission developing effective prevention control strategies. As part the Grand Challenges Explorations: New Approaches Characterize Global Burden Antimicrobial Resistance Program, we present genomic WHO carbapenemase-resistant, ESBL-producing, and/or colistin-resistant Escherichia...
Background Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild domestic mammals across Central South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas cruzi. We have sequenced T. genome to provide new tools elucidating distinct intriguing biology this species key pathways related interaction with its arthropod mammalian hosts. Methodology/Principal Findings The haploid ∼24 Mb in length, smallest least...
Antimicrobial resistance (AMR) poses a significant threat to global public health. The One Health approach, which integrates human, animal, and environmental health, highlights the roles of agricultural hospital settings in propagation AMR. This study aimed analyze resistome gut microbiome composition individuals from high-intensity animal husbandry area western region Santa Catarina, Southern Brazil, who were subsequently admitted University Hospital city Florianopolis, located eastern part...
Mother's own milk (MOM) is the most complete nutritional resource for newborns. In cases where mothers are unable to produce sufficient or cannot breastfeed, preferred alternative pasteurized donor human (PDM), which routinely provided by banks. PDM offers a superior range of and immunological elements compared any commercially available formula. However, ensure biosafety, undergoes pasteurization, process that inactivates commensal microbiota reduces certain bioactive compounds. This study...
Reports of Gram-negative bacteria harboring multiple carbapenemase genes have increased in South America, leading to an urgent need for appropriate microbiological diagnosis. We evaluated phenotypic methods detecting Klebsiella pneumoniae 2 (KPC-2) and New Delhi metallo-β-lactamase-1 (NDM-1) coexpression members the K. complex (i.e., pneumoniae, quasipneumoniae, variicola) isolated from human animal hosts, based on inhibition ceftazidime-avibactam (CZA) aztreonam (ATM) by dipicolinic acid...
Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening difficult to treat. Here, we investigate for first time transcriptome of clinically relevant cysticerci form.Using Expressed Sequence Tags (ESTs) produced ORESTES method, a total 1,520 high quality ESTs were generated 20 cDNA mini-libraries its analysis revealed...
Two species of the genus Trypanosoma infective to humans have been extensively studied at a cell and molecular level, but study third, rangeli, remains in relative infancy. T. rangeli is non-pathogenic, frequently mistaken for related Chagas disease agent cruzi with which it shares vectors, hosts, significant antigenicity sympatric distribution over wide geographical area. In this study, we present gene expression profile as determined by generation ESTs (Expressed Sequence Tags) ORESTES...
When the FLT3 gene is mutated, it originates a modified receptor with structural changes, which give survival advantage and malignant hematopoietic cell proliferation. Thus, presence of mutations in this considered an unfavorable prognostic factor. A total 85 consecutive samples newly diagnosed untreated patients AL were included study after they provided their informed consent. detected by PCR. For pediatric group, positive correlation was observed between WBC count FLT3-ITD AML ALL....
Mobile genetic elements contribute to the emergence and spread of multidrug-resistant bacteria by enabling horizontal transfer acquired antibiotic resistance among different bacterial species genera. This study characterizes backbone
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray identify papilloma virus in cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology another collected using BD SurePath™ kit HPV tests. Eighty samples (24.6%) positive gene by...
Assessment of the genetic variability and population structure Trypanosoma rangeli, a non-pathogenic American trypanosome, was carried out through microsatellite single-nucleotide polymorphism analyses. Two approaches were used for typing: data mining in expressed sequence tag /open reading frame tags libraries PCR-based Isolation Microsatellite Arrays from genomic libraries. All microsatellites found evaluated their abundance, frequency usefulness as markers. Genotyping T. rangeli strains...
Plasmid-mediated polymyxin resistance has become a global health concern, not only because its dissemination occurred drastically but also it begun to be reported in multidrug-resistant (MDR) pathogens. We hereby report microbiological and genomic characteristics of two mcr-1.1-positive polymyxin-resistant Escherichia coli isolates identified for the first time community patients, Santa Catarina, Southern Brazil. E. strains belonging ST206 ST354 resistome analysis revealed presence...
Hospital-built environment colonization by healthcare-associated infections-related bacteria (HAIrB) and the interaction with their occupants have been studied to support more effective tools for HAI control. To investigate HAIrB dynamics antimicrobial resistance (AMR) profile we carried out a 6-month surveillance program in developing country public hospital, targeting patients, hospital environment, healthcare workers, using culture-dependent culture-independent 16S rRNA gene sequencing...
The Klebsiella pneumoniae carbapenemase (KPC) is disseminated worldwide mostly by plasmids. However, in Pseudomonas aeruginosa chromosomal mutations are more frequently responsible for resistance to carbapenems than the acquisition of mobile elements harbouring carbapenemases genes. Indeed, although uncommon, KPC-2-producing P. has appeared frequently, including Brazil. Here we report first genomic analysis a plasmid-mediated KPC-2 an extensively drug-resistant (XDR) isolated Santa Catarina,...
This study shows the characterization of histone H2A intergenic region sequences (H2A IR) from Trypanosoma rangeli KP1(+) and KP1(-) strains isolated distinct hosts geographic regions. Also, a comparative unweighted pair-group method using arithmetic averages (UPMGA) analysis with polymerase chain reaction profiles 24Salpha rDNA miniexon genes was performed. Detailed IR sequence revealed discrete size polymorphism among T. presence single-nucleotide polymorphisms minisatellite repeats,...
Glycoconjugates play essential roles in cell recognition, infectivity and survival of protozoan parasites within their insect vectors mammalian hosts. β-galactofuranose is a component several glycoconjugates many organisms, including variety trypanosomatids, but absent African trypanosomes. Herein, we describe the presence β(1-3) galactofuranosyl transferase (GALFT), an important enzyme galactofuranose biosynthetic pathway, Trypanosoma rangeli. The T. rangeli GALFT gene (TrGALFT) has ORF 1.2...