A5032241534- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- Lipoproteins and Cardiovascular Health
- COVID-19 epidemiological studies
- Systemic Lupus Erythematosus Research
- Health Systems, Economic Evaluations, Quality of Life
- Autoimmune and Inflammatory Disorders Research
- Clinical practice guidelines implementation
- Immune Cell Function and Interaction
- Rheumatoid Arthritis Research and Therapies
Amsterdam University Medical Centers
2022
University of Amsterdam
2022
Centre for Inflammation Research
2019-2021
King's College London
2019-2021
Durham County Department of Public Health
1998
Antibody responses to SARS-CoV-2 can be detected in most infected individuals 10-15 d after the onset of COVID-19 symptoms. However, due recent emergence human population, it is not known how long antibody will maintained or whether they provide protection from reinfection. Using sequential serum samples collected up 94 post symptoms (POS) 65 with real-time quantitative PCR-confirmed infection, we show seroconversion (immunoglobulin (Ig)M, IgA, IgG) >95% cases and neutralizing when sampled...
Understanding of the true asymptomatic rate infection SARS-CoV-2 is currently limited, as understanding population-based seroprevalence after first wave COVID-19 within UK. The majority data thus far come from hospitalised patients, with little focus on general population cases, or their symptoms.We undertook enzyme linked immunosorbent assay characterisation IgM and IgG responses against spike glycoprotein nucleocapsid protein 431 unselected general-population participants TwinsUK cohort...
There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not the capacity develop these and/or manual processing. We provide multiple workflows nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind DNA/RNA 96 (Omega Bio-tek). also compared One-step RT-qPCR reagents: TaqMan...
<h3>Abstract</h3> <b>Objective:</b> To compare the implications of four widely used cholesterol screening and treatment guidelines by applying them to a population in United Kingdom. <b>Design:</b> Guidelines were applied based data from cross sectional study cardiovascular disease risk factors. <b>Setting:</b> Newcastle upon Tyne,United <b>Subjects:</b> General sample (predominantly European origin) 322 men 319 women aged 25-64 years. <b>Main outcome measures:</b> Proportions...
The expression of anti-inflammatory IL-10 by CD4+ T cells is indispensable for immune homeostasis, as it allows to moderate their effector function. We previously showed that TNF-α blockade during cell stimulation in cell/monocyte cocultures resulted maintenance IL-10-producing and identified IKZF3 a putative regulator IL-10. In this study, we tested the hypothesis transcriptional using human cell-only culture system. IL-10+ expressed highest levels both ex vivo after activation compared...
Abstract There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not the capacity develop these and/or manual processing. We provide multiple workflows nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind DNA/RNA 96 (Omega Bio-tek). also compared One-step RT-qPCR...
Abstract TNF‐blockade has shown clear therapeutic value in rheumatoid arthritis and other immune‐mediated inflammatory diseases, however its mechanism of action is not fully elucidated. We investigated the effects on CD4 + T cell activation, maturation, proliferation, assessed whether TNF‐inhibitors confer regulatory potential to cells. CyTOF flow cytometry analysis revealed that vitro treatment human cells with anti‐TNF monoclonal antibody adalimumab promoted IL‐10 expression cells, whilst...
Rheumatoid arthritis (RA) is a chronic autoimmune disease of unknown etiology characterized by inflammation the peripheral synovial joints leading to pannus formation and bone destruction. Factor (RF) anti-citrullinated protein antibodies (ACPA) are present years before clinical manifestations indicative break in tolerance that precedes inflammation. The majority studies investigating pathogenesis focus on joint as target site while few explore initial which occurs within secondary lymphoid...