A5076186657- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- Liver Disease Diagnosis and Treatment
- Biosensors and Analytical Detection
- Pneumocystis jirovecii pneumonia detection and treatment
- Pneumonia and Respiratory Infections
- COVID-19 and healthcare impacts
- HIV/AIDS Research and Interventions
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- HIV Research and Treatment
- Malaria Research and Control
- HIV/AIDS drug development and treatment
- Bacteriophages and microbial interactions
- COVID-19 epidemiological studies
- Hepatitis Viruses Studies and Epidemiology
- Tuberculosis Research and Epidemiology
- Mosquito-borne diseases and control
- COVID-19 Impact on Reproduction
Guy's and St Thomas' NHS Foundation Trust
2016-2025
King's College London
2015-2023
St Thomas' Hospital
2016-2023
National Health Service
2023
International Severe Acute Respiratory and Emerging Infection Consortium
2023
Oxford Nanopore Technologies (United Kingdom)
2023
The London College
2023
St. Thomas Hospital
2023
St Mary's Hospital
2023
Thomas Foundation
2022
Antibody responses to SARS-CoV-2 can be detected in most infected individuals 10-15 d after the onset of COVID-19 symptoms. However, due recent emergence human population, it is not known how long antibody will maintained or whether they provide protection from reinfection. Using sequential serum samples collected up 94 post symptoms (POS) 65 with real-time quantitative PCR-confirmed infection, we show seroconversion (immunoglobulin (Ig)M, IgA, IgG) >95% cases and neutralizing when sampled...
Cases of human monkeypox are rarely seen outside west and central Africa. There few data regarding viral kinetics or the duration shedding no licensed treatments. Two oral drugs, brincidofovir tecovirimat, have been approved for treatment smallpox demonstrated efficacy against in animals. Our aim was to describe longitudinal clinical course a high-income setting, coupled with dynamics, any adverse events related novel antiviral therapies.
Abstract Objective To characterise the clinical features of monkeypox infection in humans. Design Descriptive case series. Setting A regional high consequences infectious disease centre with associated primary and secondary care referrals, affiliated sexual health centres south London between May July 2022. Participants 197 patients polymerase chain reaction confirmed infection. Results The median age participants was 38 years. All were men, 196 identified as gay, bisexual, or other men who...
Abstract Antibody (Ab) responses to SARS-CoV-2 can be detected in most infected individuals 10-15 days following the onset of COVID-19 symptoms. However, due recent emergence this virus human population it is not yet known how long these Ab will maintained or whether they provide protection from re-infection. Using sequential serum samples collected up 94 post symptoms (POS) 65 RT-qPCR confirmed SARS-CoV-2-infected individuals, we show seroconversion >95% cases and neutralizing antibody...
SummaryBackgroundLateral flow devices (LFDs) for rapid antigen testing are set to become a cornerstone of SARS-CoV-2 mass community testing, although their reduced sensitivity compared with PCR has raised questions how well they identify infectious cases. Understanding capabilities and limitations is, therefore, essential successful implementation. We evaluated six commercial LFDs assessed correlation virus culture cycle threshold (Ct) values.MethodsIn single-centre, laboratory evaluation...
There is a clear requirement for an accurate SARS-CoV-2 antibody test, both as complement to existing diagnostic capabilities and determining community seroprevalence. We therefore evaluated the performance of variety testing technologies their potential use tools. Highly specific in-house ELISAs were developed detection anti-spike (S), -receptor binding domain (RBD) -nucleocapsid (N) antibodies used cross-comparison ten commercial serological assays—a chemiluminescence-based platform, two...
Abstract Background A cost effective and efficient diagnostic tool for COVID-19 as near to the point of care (PoC) possible would be a game changer in current pandemic. We tested reverse transcription loop mediated isothermal amplification (RT-LAMP), method which can produce results under 30 min, alongside standard methods real-life clinical setting. Methods This prospective service improvement project piloted an RT-LAMP on nasal pharyngeal swabs 21 residents high dependency home, with two...
During the rapidly evolving novel coronavirus disease 2019 (COVID-19) outbreak, more than a million cases have been diagnosed worldwide; however, there limited reports of HIV and COVID-19 coinfection at time writing. We report case highlighting challenge diagnosing Pneumocystis jirovecii pneumonia (PJP) in an individual with HIV. A 55-year-old male presented 7-day history fevers, cough, hypoxia. Comorbidities include well controlled infection (diagnosed 2006, current CD4+ cell count 422...
Viral diversity presents an ongoing challenge for diagnostic tests, which need to accurately detect all circulating variants. The Abbott Global Surveillance program monitors severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants and their impact on test performance.
Some people who are receiving dialysis treatment have virus infection such as hepatitis B, C and/or HIV that is present in their blood. These infections can be transmitted to other patients if blood contaminated by the of another with a viral infection. Haemodialysis performed passing from patient through machine, and multiple receive within unit. Therefore, there risk these viruses may around session. This documents sets out recommendations for minimising this risk.There sections describing...
Background: To date, although neonatal infections with severe acute respiratory syndrome coronovirus 2 (SARS-CoV-2) have been described, none of these proven to be the result vertical transmission SARS-CoV-2. Methods: We describe probable SARS-CoV-2 in a neonate born mother coronavirus disease 2019 (COVID-19). Results: Following cesarean section, was kept strict isolation. Molecular tests for on samples, blood, and meconium were initially negative, but positive nasopharyngeal aspirate third...
Antibodies to SARS-CoV-2 serve as critical diagnostic markers for determining how broadly the COVID-19 pandemic has spread, confirming patient recovery, monitoring potential long-term effects of infection, and evaluating protection from reinfection. As new antibody tests become available, it is important evaluate their performance utility. The aim this study was compare Abbott PanbioTM IgG/IgM Rapid Test Device against ArchitectTM SARS CoV-2 IgG Assay detection antibody.Two panels specimens...
Background Rapid identification of individuals with acute respiratory infections is crucial for preventing nosocomial infections. For rapid diagnosis, especially in EDs, lateral flow devices (LFDs) are a convenient, inexpensive option turnaround. Several ‘multiplex’ LFDs (M-LFDs) now exist, testing multiple pathogens from single swab sample. We evaluated the real-world performance M-LFD versus PCR detecting influenza A, B and SARS-CoV-2) ED setting. Methods After preliminary evaluation an...
The prevalence of hepatitis is high in emergency department (ED) attendees the United Kingdom, with a up to 2% for B (HBV) HBsAg, and 2.9% C (HCV) RNA. aim this paper perform an economic evaluation opt-out ED-based HCV HBV testing.A Markov model was developed analyze cost-effectiveness testing EDs UK. used data from UK studies ED parameterize (1.4% RNA, 0.84% HBsAg), test costs, intervention effects (contact rates linkage care). For HCV, we antibody cost £3.64 RNA £68.38, assumed...
An evaluation of a rapid portable gold-nanotechnology measuring SARS-CoV-2 IgM, IgA and IgG antibody response to spike 1 (S1), 2 (S) nucleocapsid (N) antigens using serum from 74 RNA(+) patients 47 control patients.
Acute renal failure is a common complication of severe malaria in adults, and without replacement therapy (RRT), it carries poor prognosis. Even when RRT available, delaying its initiation may increase mortality. Earlier identification patients who will need improve outcomes. Prospectively collected data from two intervention studies adults with were analysed focusing on laboratory features presentation their association later requirement for RRT. In particular, indices acute tubular...
Therapies that halt progression of chronic hepatitis B virus (HBV) and achieve a cure for C (HCV) have encouraged development innovative strategies to diagnose link patients care. We describe the prevalence risk factors HBV HCV infections use an opt-out testing integrated linkage care pathway in London Emergency Department (ED). ED aged ≥16 years having routine blood tests from 15 February-28 March 2016 were tested hepatitis, unless opted out. Hepatitis surface antigen (HBsAg) antibody...
Abstract There is a clear requirement for an accurate SARS-CoV-2 antibody test, both as complement to existing diagnostic capabilities and determining community seroprevalence. We therefore evaluated the performance of variety testing technologies their potential tools. A highly specific in-house ELISA was developed detection anti-spike (S), -receptor binding domain (RBD) -nucleocapsid (N) antibodies used cross-comparison ten commercial serological assays – chemiluminescence-based platform,...
Abstract Background The contribution of HIV to COVID‐19 outcomes in hospitalized inpatients remains unclear. We conducted a multi‐centre, retrospective matched cohort study SARS‐CoV‐2 PCR‐positive hospital analysed by status. Methods HIV‐negative patients were people living with (PLWH) admitted from 1 February 2020 31 May up 3:1 ratio the following: site, test date ± 7 days, age 5 years, gender, and index multiple deprivation decile 1. primary objective was clinical improvement (two‐point or...
Abstract Background The need for a fast and reliable test COVID-19 is paramount in managing the current pandemic. A cost effective efficient diagnostic tool as near to point of care (PoC) possible would be game changer testing. We tested reverse transcription loop mediated isothermal amplification (RT-LAMP), method which can produce results under 30 minutes, alongside standard methods real-life clinical setting. Methods This service improvement project piloted research RT-LAMP on nasal...
Innovative testing approaches and care pathways are required to meet global hepatitis B virus (HBV) C (HCV) elimination goals. Routine blood-borne (BBV) in emergency departments (EDs) high-prevalence areas is suggested by the European Centre for Disease Prevention Control (ECDC) but there limited evidence this. Universal HIV our ED according UK guidance has been operational since 2015. We conducted a real-world service evaluation of modified electronic patient record (EPR) system include...
Abstract Background Clinical metagenomics involves the genomic sequencing of all microorganisms in clinical samples ideally after depletion human DNA to increase sensitivity and reduce turnaround times. Current methods preferentially preserve either or RNA containing microbes, but not both simultaneously. Here we describe present data using a practical rapid mechanical host-depletion method allowing simultaneous detection linked with nanopore sequencing. Methods The cells from respiratory...