A5027246981- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Plasmonic and Surface Plasmon Research
- Orbital Angular Momentum in Optics
- Near-Field Optical Microscopy
- Force Microscopy Techniques and Applications
- Quantum Dots Synthesis And Properties
- Photoacoustic and Ultrasonic Imaging
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- Delphi Technique in Research
- Conferences and Exhibitions Management
- Galectins and Cancer Biology
- Interdisciplinary Research and Collaboration
- Microfluidic and Bio-sensing Technologies
- Laser-Ablation Synthesis of Nanoparticles
- Cardiomyopathy and Myosin Studies
- Thermal Radiation and Cooling Technologies
- Electrowetting and Microfluidic Technologies
- Nanomaterials and Printing Technologies
- Career Development and Diversity
- Advanced Thermodynamics and Statistical Mechanics
Consejo Nacional de Investigaciones Científicas y Técnicas
2019-2025
London Centre for Nanotechnology
2022-2024
University College London
2022-2024
Fundación Ciencias Exactas y Naturales
2018-2021
University of Buenos Aires
2018-2021
Early career researchers (ECRs) are important stakeholders leading efforts to catalyze systemic change in research culture and practice. Here, we summarize the outputs from a virtual unconventional conference (unconference), which brought together 54 invited experts 20 countries with extensive experience ECR initiatives designed improve practice of science. Together, drafted 2 sets recommendations for (1) ECRs directly involved or activities practice; (2) who wish support these efforts....
The development of efficient nanoscale photon absorbers, such as plasmonic or high-index dielectric nanostructures, allows the remotely controlled release heat on using light. These photothermal nanomaterials have found applications in various research and technological fields, ranging from materials science to biology. However, measuring thermal fields remains an open challenge, hindering full comprehension control phenomena. Here, we review discuss existent thermometries suitable for...
Silicon nanoparticles possess unique size-dependent optical properties due to their strong electric and magnetic resonances in the visible range. However, widespread application has been limited, comparison with other (e.g., metallic) nanoparticles, because preparation on monodisperse colloids remains challenging. Exploiting of Si nano- microdevices calls for methods able sort organize them from a colloidal suspension onto specific positions solid substrates nanometric precision. We...
Abstract Fluorescence nanoscopy and single‐molecule methods are entering the realm of structural biology, breaking new ground for dynamic measurements at room temperature liquid environments. Here, localization microscopy, polarization‐dependent excitation, protein engineering combined to determine orientation a fluorophore forming hydrogen bonds inside cavity. The observed conformations in good agreement with molecular dynamics simulations, enabling new, more realistic interplay between...
We present Alpha-BET, a structure-guided strategy leveraging AlphaFold to identify optimal ALFAtag insertion sites for minimally disruptive labeling of viral glycoproteins with high-affinity nanobodies. Applied HIV-1 Env, SARS-CoV-2 S, and Nipah G, Alpha-BET preserves structural integrity function. For we demonstrate super-resolution DNA-PAINT MINFLUX 3D imaging enabled by tag insertion, showcasing its power visualizing native trimers in single virions potential broader virological applications.
Protein tyrosine phosphatase non-receptor type 22 (PTPN22) is a critical regulator of T cell signaling, working in concert with C-terminal SRC kinase (Csk) to dephosphorylate key signaling proteins and suppress lymphocyte activation. The R620W variant PTPN22, one the most prevalent mutations associated autoimmune diseases, has been implicated altered responses, although its broader effects on activation are not fully understood. Recent studies have uncovered novel interaction between PTPN22...
Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging Lifetime Imaging Microscopy (FLIM) enable visualization spatial distribution molecular functional states. However, conventional FLIM provide average information over an ensemble molecules within a diffraction-limited volume, which limits information, accuracy, dynamic range observed signals. Here, approach to obtain...
While colloidal chemistry provides ways to obtain a great variety of nanoparticles with different shapes, sizes, material compositions, and surface functions, their controlled deposition combination on arbitrary positions substrates remain considerable challenge. Over the last ten years, optical printing arose as versatile method achieve this purpose for kinds nanoparticles. In article, we review state art single discuss its strengths, limitations, future perspectives by focusing four main...
ABSTRACT Single molecule localization microscopy (SMLM) is constrained by selective illumination configurations to achieve high signal-to-background ratio (SBR), forcing trade-offs between penetration depth, field-of-view (FOV), and spatial resolution. We demonstrate that a Spinning Disc confocal microscope with Optical Photon Reassignment (SDC-OPR) in combination DNA-PAINT effectively balances these limitations. This system enables high-resolution imaging through multiple cellular layers,...
Current methods for determining equilibrium constants often operate in three-dimensional environments, which may not accurately reflect interactions with membrane-bound proteins. With our technique, based on single-molecule localization microscopy (SMLM), we directly determine protein-protein association (
Early career researchers (ECRs) are important stakeholders leading efforts to catalyze systemic change in the conduct and communication of science. Here, we summarize outputs from a virtual unconventional conference (unconference), which brought together 54 invited experts 20 countries with extensive experience ECR initiatives designed improve The event was focused on why ECRs needed science obstacles they face when trying promote reform. Our discussions also highlighted additional that...
The elucidation of complex biological processes often requires monitoring the dynamics and spatial organization multiple distinct proteins organized on sub-micron scale. This length scale is well below diffraction limit light, as such not accessible by classical optical techniques. Further, high molecular concentrations found in living cells, typically micro- to mili-molar range, preclude single-molecule detection confocal volumes, essential quantify affinity constants protein-protein...
En este trabajo presentamos la implementación de un novedoso método microscopía 3D basado en el barrido orbital haz excitación alrededor estructura interés. Esta tecnología es capaz producir imágenes tridimensionales estructuras movimiento con resolución nanométrica y pocas decenas milisegundos. Dado que intensidad luz emitida por objeto fluorescente depende fuertemente distancia entre superficie del mismo, modulando haz-objeto, conociendo perfil excitación, posible recuperar forma interés a...
Surfactant-free silicon nanoparticles of a predefined and narrow (σ < 10 nm) size range can be selectively immobilized on substrate by optical printing from polydisperse colloidal suspension. The selectivity is based differential forces that applied different sized tuning the light wavelength to their size-dependent magnetic dipolar resonance.
Silicon nanoparticles possess unique size-dependent optical properties due to their strong electric and magnetic resonances in the visible range. However, widespread application has been limited, comparison other (e.g. metallic) nanoparticles, because preparation on monodisperse colloids remains challenging. Exploiting of Si nano- micro-devices calls for methods able sort organize them from a colloidal suspension onto specific positions solid substrates with nanometric precision. Here, we...
FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging FLIM (Fluorescence Lifetime Imaging Microscopy) enable visualization spatial distribution molecular functional states. However, conventional provide average information over an ensemble molecules within diffraction-limited volume, which limits information, accuracy, dynamic range observed signals. Here, we demonstrate approach to obtain super-resolved based on...