A5100399820- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Osteoarthritis Treatment and Mechanisms
- DNA Repair Mechanisms
- Eicosanoids and Hypertension Pharmacology
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Alcohol Consumption and Health Effects
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- Drug-Induced Hepatotoxicity and Protection
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory mediators and NSAID effects
- T-cell and B-cell Immunology
- Cancer-related Molecular Pathways
- MXene and MAX Phase Materials
- Mesenchymal stem cell research
- Heme Oxygenase-1 and Carbon Monoxide
- Cell death mechanisms and regulation
- Wound Healing and Treatments
Shanghai Ninth People's Hospital
2023
Shanghai Jiao Tong University
2023
First Affiliated Hospital of GuangXi Medical University
2022
Guangxi Medical University
2022
First Affiliated Hospital of Heilongjiang University of Chinese Medicine
2021
Capital Medical University
2017-2021
Beijing YouAn Hospital
2017-2021
Beijing Friendship Hospital
2018-2020
Microglia are the resident immune cells in central nervous system and play an essential role brain homeostasis neuroprotection diseases. Exosomes crucial intercellular communication by transporting bioactive miRNAs. Thus, this study aimed to investigate function of microglial exosome presence ischemic injury related mechanism. Oxygen-glucose deprivation (OGD)-treated neurons transient middle cerebral artery occlusion (TMCAO)-treated mice were applied study. Western blotting, RT-PCR, RNA-seq,...
Background: Interleukin-1β (IL-1)-treated mesenchymal stem cells (MSCs) and IL-1-MSCs-conditioned medium (CM) exert anti-inflammatory roles. Astrocytes are essential for the modulation of synaptic activity neuronal homeostasis in brain. Exosomes critical mediators intercellular communication. However, mechanism underlying effect IL-1-treated MSCs remains unknown. Methods: In this study, exosomes (IL-1-Exo) were isolated from MSCs. addition, lipopolysaccharide (LPS)-treated hippocampal...
The biogenesis and diagnostic value of exosomes in nonalcoholic fatty liver disease (NAFLD) are unclear. In this study, we revealed that the plasma exosome level was higher patients with NAFLD than healthy controls. Damage-regulated autophagy modulator (DRAM) identified as one genes related to secretion NAFLD. Then, loss or knockdown DRAM down-regulated from hepatic cells using a knockout mouse model cell model. reversed high-fat diet–induced increase secreted exosomes. Furthermore,...
Vascular calcification (VC) is the most widespread pathological change in diseases of vascular system. However, we do not have a good understanding molecular mechanisms and effective therapeutic approaches for VC. Curcumin (CUR) natural polyphenolic compound that has hypolipidemic, anti-inflammatory, antioxidant effects on cardiovascular Exosomes are known to extensive miRNAs intercellular regulation. This study investigated whether CUR attenuates VC by affecting secretion exosomal miRNAs....
Background: Treatment of diabetic wounds is a major challenge in clinical practice. Extracellular vesicles (EVs) from adipose-derived stem cells have shown effectiveness wound models. However, obtaining ADSC-EVs requires culturing vast numbers cells, which hampered by the need for expensive equipment and reagents, extended time cost, complicated procedures before commercialization. Therefore, methods to extract EVs discarded tissue be developed, immediate application during surgery. For this...
Abstract CD4 + T-cell-converted − CD8 double negative (cDNT) have strong suppressive activity in the maintenance of immune tolerance, whereas IL-2 promotes cDNT proliferation and enhances resistance to apoptosis. However, intrinsic mechanisms that regulate survival are still unknown. Here we demonstrate OX40 molecule was highly expressed on cDNT. The expression necessary promote inhibit apoptosis vivo vitro. promoted by regulating Bcl-2, Bcl-xL, Survivin, BCL2L11. Canonical NF-κB cell...
DNA damage-regulated autophagy modulator 1 (DRAM1) could play important roles in inflammation and hepatic apoptosis, while its alcohol-related liver disease (ALD), which is characterized by are still unclear. In this study, we explored the expression, role, mechanism of DRAM1 ALD. Firstly, our results showed that was significantly increased tissues mice at early stage alcohol treatment. addition, knockout reduced, liver-specific overexpression aggravated, alcohol-induced steatosis, injury,...
The mitochondrial toxicity of nucleoside reverse transcriptase inhibitors (NRTIs) is due to the inhibition DNA (mtDNA) polymerase γ (pol γ). Previous studies have shown that wild type p53 (wtp53) can interact with pol and mtDNA enhance base excision repair (mtBER) activity increase accuracy synthesis. N-terminal transactivation domain central specific DNA-binding play critical roles in stimulation BER. In this study, we identified possible wtp53, Δ40p53 Δ133p53 regulating cells d4T...
Background and Purpose The innate adaptive immune systems both play important roles in drug‐induced liver injury (DILI). However, the crosstalk between immunity DILI is largely unknown. Extensive likely mandated by co‐stimulatory interactions these systems. OX40 a molecule, but whether it regulates intrahepatic response remains Experimental Approach Acute was induced paracetamol (acetaminophen), carbon tetrachloride (CCl 4 ), d ‐galactosamine/LPS (GalN/LPS) wild‐type (WT) Ox40 knockout (KO)...
Abstract Background ： Cartilage tissue engineering is a promising option for repairing cartilage defects caused by trauma, inflammation and osteoarthritis, although harvesting large number of seeding cells with stable phenotypes remains major challenge. stem/progenitor (CSPCs) seem to be cell source. Hypoxic extracellular vesicles secreted mesenchymal stem may play role in cell-cell tissue-tissue communication transporting various RNAs proteins cell-based therapy. In the current study, we...