- Weed Control and Herbicide Applications
- Pesticide and Herbicide Environmental Studies
- Allelopathy and phytotoxic interactions
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- Legume Nitrogen Fixing Symbiosis
- Polyamine Metabolism and Applications
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant and fungal interactions
- Nematode management and characterization studies
- Genetics and Plant Breeding
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Pharmaceutical and Antibiotic Environmental Impacts
- Insect Resistance and Genetics
- Soil Carbon and Nitrogen Dynamics
- NF-κB Signaling Pathways
- Plant responses to water stress
- Pesticide Residue Analysis and Safety
- Plant Disease Management Techniques
- Fungal Plant Pathogen Control
Hunan Agricultural University
2019-2025
Nanjing Agricultural University
2014-2024
Hunan Academy of Agricultural Sciences
2019-2021
Sichuan Agricultural University
2018-2021
The University of Western Australia
2019-2021
Biotechnology Research Institute
2020-2021
Farmland Irrigation Research Institute
2019
The Ohio State University
2008
Significance Glyphosate is the world’s dominantly used herbicide to control weedy plant species in a wide range of situations, especially global field crops soybean, maize, canola, and cotton with genetically engineered glyphosate resistance. Persistent selection has led worldwide evolution glyphosate-resistant weeds. Several biochemical physiological mechanisms have been identified that endow To be toxic plants, must present cytoplasm, thus reducing cytoplasmic sublethal level could confer...
Glyphosate, the most commonly used herbicide in world, controls a wide range of plant species, mainly because plants have little capacity to metabolize (detoxify) glyphosate. Massive glyphosate use has led world-wide evolution glyphosate-resistant (GR) weed including economically damaging grass
Frequent herbicide use selects for resistance in weeds. Cytochrome P450s are important detoxification enzymes responsible plants. We identified and characterized a candidate P450 gene (BsCYP81Q32) from the problematic weed Beckmannia syzigachne to test whether it conferred metabolic acetolactate synthase-inhibiting herbicides mesosulfuron-methyl, bispyribac-sodium, pyriminobac-methyl. Transgenic rice overexpressing BsCYP81Q32 was resistant three herbicides. Equally, ortholog OsCYP81Q32 more...
Introduction Bromus japonicus is one of the most notorious agricultural weeds in China. The long-term use ALS-inhibiting herbicides has led to rapid evolution herbicide resistance B. . population (BJ-R) surviving mesosulfuron-methyl treatment was collected from wheatland. Here, we aimed confirm mechanisms this putative resistant population. Methods dose-reponse tests were used test level herbicides. Pretreatment with P450 and GST inhibitors activity assays determine whether or involved BJ-R...
The regenerative capacity of the embryonic callus, a complex quantitative trait, is one main limiting factors for maize transformation. This trait was decomposed into five traits, namely, green callus rate (GCR), differentiating (CDR), plantlet number (CRN), rooting (CRR) and browning (CBR). To dissect genetic foundation transformation, in this study multi-locus genome-wide association studies (GWAS) traits were performed population 144 inbred lines genotyped with 43,427 SNPs. Using...
Non-target site resistance (NTSR) to herbicides is an increasing concern for weed control. Metabolic herbicide important mechanism NTSR. However, little known about metabolic at the genetic level. In this study, we have identified three fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.) populations, in which molecular basis NTSR remains unclear. To reveal mechanisms of resistance, genes likely be involved metabolism (e.g. cytochrome P450s, esterases, hydrolases,...
Asia minor bluegrass (Polypogon fugax) is one of the main weeds invading Chinese canola fields. The P. fugax resistant population SC-R, which survived quizalofop-p-ethyl at field-recommended rate (67.5 g ha-1 ), was collected from a field in Qingsheng County China. present study aimed to (1) characterize SC-R resistance pattern acetyl-CoA carboxylase (ACCase)-inhibiting herbicides, and (2) investigate mechanism this population.Dose-response studies showed that haloxyfop occurred population....
Alkaline soil has a high pH due to carbonate salts and usually causes more detrimental effects on crop growth than saline soil. Sodium hydrogen exchangers (NHXs) are pivotal regulators of cellular Na + /K homeostasis, which is essential for salt tolerance; however, their role in alkaline tolerance largely unknown. Therefore, this study, we investigated the function soybean NHX gene, GmNHX6 , plant response stress. encodes Golgi-localized sodium/hydrogen exchanger, its transcript abundance...
Eleusine indica has become a global nuisance weed and evolved resistance to glufosinate. The involvement of target-site (TSR) in glufosinate E. been elucidated, while the role nontarget-site (NTSR) remains unclear. Here, we identified glufosinate-resistant (R) population that is highly resistant glufosinate, with index 13.5-fold. Molecular analysis indicated mechanism this R does not involve TSR. In addition, pretreatment two known metabolic enzyme inhibitors, cytochrome P450 (CYP450)...
The emergence of 4-hydroxyphenylpyruvate dioxygenase (HPPD) herbicides as efficacious target-site has been noteworthy. In recent years, only four species broadleaf weeds have developed resistance due to the long-term widespread use HPPD herbicides. This study represents first reported instance a grass weed exhibiting inhibitors. We identified new HPPD-resistant Chinese sprangletop [Leptochloa chinensis (L.) Nees] population (R population). At recommended dose tripyrasulfone, inhibition rate...
Abstract BACKGROUND Resistance to multiple herbicides is common in Lolium rigidum . Here, resistance acetolactate synthase (ALS)‐ and susceptibility acetyl‐CoA carboxylase (ACCase)‐inhibiting was confirmed a glyphosate‐resistant L. population (NLR70) from Australia the mechanisms of pyroxsulam were examined. RESULTS No ALS target‐site mutations nor gene overexpression detected. Cytochrome P450 monooxygenase (P450) glutathione S‐transferase (GST) inhibitors (indicators some certain P450s or...
Mutagenesis breeding, combined with the application of corresponding herbicides to develop herbicide-resistant rice germplasm, provides great promise for management weeds and weedy rice. In this study, a topramezone-resistant mutant, TZR1, was developed from indica line Chuangyu 9H (CY9H) through radiation mutagenesis topramezone selection. Dose–response curves revealed that resistance index TZR1 1.94-fold compared CY9H. The mechanism not due target-site resistance. This could be reversed by...
Abstract BACKGROUND Polypogon fugax has evolved resistance to multiple herbicides in China, yet there been no documented case of glyphosate resistance. A putative glyphosate‐resistant P. (HN‐R) population was collected from canola fields Hunan Province, surviving treatment at the field‐recommended rate [540 g acid equivalent (a.e.) ha −1 ]. The aims this study were elucidate level and mechanism HN‐R population. RESULTS Dose–response shikimic assays indicated that developed a low‐level (up...
Alopecurus japonicus is one of the most predominant weeds in wheat fields across China, where significant herbicide resistance has emerged over past decade. When compared to susceptible (S) population, resistant (R) population exhibited a 9.48-fold increase fenoxaprop-P-ethyl. The R displayed cross-resistance haloxyfop-P-methyl, quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim, clethodim and pinoxaden. No known mutations or overexpression ACCase were detected population. showed enhanced...
Capsella bursa-pastoris, a prevalent wheat-field weed in China, demonstrates substantial resistance to tribenuron-methyl, herbicide-targeting acetolactate synthase (ALS). Understanding herbicide-resistance mechanisms is crucial for managing resistant populations. However, the genes potentially involved nontarget-site (NTSR) herbicide-resistant C. bursa-pastoris remain poorly understood and require further investigation. This research aimed elucidate level underlying of field population (R)...
BACKGROUND The increasing use of fenoxaprop-p-ethyl has resulted in evolved resistance American sloughgrass (Beckmannia syzigachne Steud.). Target-site-based to acetyl-CoA carboxylase (ACCase) inhibitors B. occurs owing an isoleucine-to-leucine substitution at residue 1781 (I1781L) the ACCase enzyme. A rapid detection method is needed identify resistance-conferring substitution. RESULTS Four populations that were resistant and contained I1781L identified. Conventional PCR derived cleaved...
Abstract Background Fenclorim (Fen) can effectively protect rice from pretilachlor (Pre) injury, but its effects on have not been formally evaluated; thus, the Fen mode of action for alleviating phytotoxicity caused by Pre in is clear. This study aimed to examine biochemical and physiological determine changes induced at transcriptome level. Result The chlorophyll content plants was significantly affected Fen. activity oxidative stress enzymes showed that did elicit any stress; however, it...
Summary B eckmannia syzigachne ( A merican sloughgrass) is a competitive grass weed found in China. Fenoxaprop‐P‐ethyl widely used for control of this species Resistance to fenoxaprop‐P‐ethyl . has been reported be conferred by an isoleucine(Ile)‐1781‐leucine(Leu) substitution the gene encoding herbicide target, acetyl‐CoA carboxylase ACC ase). In study, three mutations were detected derived cleaved amplified polymorphic sequence dCAPS ) method fenoxaprop‐P‐ethyl‐resistant populations:...
Abstract Chinese sprangletop [ Leptochloa chinensis (L.) Nees] is one grass weed severely affecting rice ( Oryza sativa L.) growth in paddies China. Cyhalofop-butyl the main herbicide used to control weeds paddy fields, especially for controlling L. ; however, has evolved resistance cyhalofop-butyl due continuous and extensive application. To investigate levels mechanisms some of areas, 66 field populations were collected treated with cyhalofop-butyl. Of these tested populations, 10 showed a...