Chronic kidney disease (CKD) is a serious public health problem characterized by progressive function loss leading to end-stage renal (ESRD) that demands dialysis or transplantation. Early detection can prevent delay progression ESRD. The study aimed gain new insights into the perturbed biochemical reactions and identify novel distinct biomarkers between ESRD CKD. Serum samples of 32 patients with (n = 13) CKD 19) were analyzed using chemical isotope labeling liquid chromatography-mass...

Dexamethasone (Dex) is a synthetic glucocorticoid (GC) drug commonly used clinically for the treatment of several inflammatory and immune-mediated diseases. Despite its broad range indications, long-term use Dex known to be associated with specific abnormalities in tissues organs. In this study, metabolomic effects on five different organs induced by chronic administration Sprague-Dawley rat model were investigated using chemical isotope labeling liquid chromatography-mass spectrometry (CIL...

Objectives This cross-sectional analytical study aims to evaluate medical students' awareness and satisfaction regarding the utilization of simulation-based learning (SBL) as a method for clinical teaching at King Saud University (KSU) over past 12 months. It seeks understand how such methods enhance self-satisfaction skills, facilitate application learned knowledge, assess role instructors in providing ample practice opportunities skills laboratory. Furthermore, levels students both...

Background: Pneumonia is a ubiquitous health condition with severe outcomes. The advancement of ultrasonography techniques allows its application in evaluating pulmonary diseases, providing safer and accessible bedside therapeutic decisions compared to chest X-ray computed tomography (CT) scan. Because aforementioned benefits, we aimed confirm the diagnostic accuracy lung ultrasound (LUS) for pneumonia adults. Methods: A systematic literature search was performed Medline, Cochrane Crossref,...

Background and Objectives: In this study, we aimed to investigate the link between common -2548G>A (rs7799039) promoter variant of human leptin gene (LEP) with serum glucose levels in obese Saudi patients. Materials Methods: A total 206 adults (80 normotensive nondiabetics, 76 hypertensive Type 2 Diabetes 50 nondiabetic controls) were genotyped for LEP polymorphism using polymerase chain reaction-restriction fragment-length technique. Results: Participants minor AA genotype had significantly...

The clinical expression of type 1 von Willebrand disease may be modified by co-inheritance other mild bleeding diatheses. We previously showed that mutations in the platelet P2Y12 ADP receptor gene (P2RY12) could contribute to phenotype patients with disease. Here we investigated whether variations G protein-coupled genes than P2RY12 also contributed phenotype. Platelet P2RY1, F2R, F2RL3, TBXA2R and PTGIR were sequenced 146 index cases potential effects identified single nucleotide assessed...

Dexamethasone is a synthetic glucocorticoid medication vastly used to treat abnormal immune responses and inflammation. Although the well-established in medical community, prolonged treatment with high dosages of dexamethasone may lead severe adverse effects through mechanisms that are not yet well-known. Lipids large class hydrophobic molecules involved energy storage, signaling, modulation gene expression, membranes. Hence, untargeted lipidomics help unravel biochemical alterations...

Nephrotic syndrome (NS) is a kidney illness characterized by excessive proteinuria, hypoalbuminemia, edema, and hyperlipidemia, which may lead to failure necessitate renal transplantation. End-stage disease, cardiovascular issues, mortality are much more common in those with NS. Therefore, the present study aimed identify potential new biomarkers associated pathogenesis diagnosis of The liquid chromatography-mass spectrometry (LC-MS) metabolomics approach was applied profile metabolome human...

Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder characterized by progressive muscle loss, leading to difficulties in movement. Mutations the DMD gene that code for protein dystrophin are responsible development of disorder, where synthesis this completely halted. Therefore, circulating could be a promising biomarker disease. Current methods diagnosing have sensitivity, specificity, and reproducibility limitations. Herein, quantitative liquid chromatography-tandem...

Cystic renal disease (CRD) comprises a heterogeneous group of genetic and acquired disorders. The cystic lesions are detected through imaging, either incidentally or after symptoms develop, due to an underlying process. In this study, we aim study the metabolomic profiles CRD patients for potential disease-specific biomarkers using unlabeled labeled metabolomics low high-resolution mass spectrometry (MS), respectively. Dried-blood spot (DBS) serum samples, collected from healthy controls,...

To study the knowledge, attitude and practice level among young Saudi adults regarding osteoporosis.A cross-sectional was done in Riyadh Saudis, within age group (17-30 years old), with sample size (663). A validated questionnaire distributed paper electronic forms. The had five parts: first part demographic variables, second source of participant knowledge about disease, third assessed osteoporosis, fourth osteoporosis attitudes, fifth for prevention osteoporosis. collected data analysed by...

Mutations in ITGA2B and ITGB3 cause Glanzmann thrombasthenia, an inherited bleeding disorder which platelets fail to aggregate when stimulated. Whereas absence of expression or qualitative defects αIIbβ3 mainly affect megakaryocytes, αvβ3 has a widespread tissue distribution. Little is known how amino acid substitutions β3 comparatively the structure both integrins. We now report computer modelling including molecular dynamics simulations extracellular head domains determine role novel...

Different laboratory methods are used to measure serum ferritin levels as a marker of iron status in the general population. This study aimed compare using enzyme-linked immunosorbent assay (ELISA) versus immunochemiluminescence (Cobas e411) and immunoturbidimetric Integra 400) terms sensitivity, specificity accuracy, whether they can be interchangeably. A comparative cross-sectional enrolled one hundred six adult Yemeni patients (33 males 73 females) aged 18-55 years, recruited from...

To assess awareness of diabetic retinopathy (DR) for Saudis with diabetes mellitus (DM) type 2 in Riyadh City.A cross-sectional study that used self-administered questionnaires conducted primary healthcare centers City between October and December 2019.There were 267 Saudi participants visiting clinics. (52.4%) aged 40 years or above at time diagnosis diabetes. (73%) had least 5 years. Most the have a good blood glucose control they do home measurements. In regard to DR, (64.4%) no...

To estimate the awareness level of Saudi adults about risk factors and warning signs cancer observe association different determinants with Knowledge in Riyadh city.A cross-sectional study carried out on 390 Saudis outpatient clinics King Khalid University Hospital (KKUH), Riyadh. Data was collected using a validated Arabic questionnaire. analyzed SPSS software.The overall participants limited, as mean score knowledge 49.2%. Most correctly answered questions were general not or warnings...

Irritable bowel syndrome is prevalent in the general population. This study investigates association between dietary intake and irritable medical college students at King Saud University besides its prevalence.This an analytical cross-sectional of 426 (271 males 155 females, age 21.21 ± 1.58 years) from 5 academic levels Medical College. A self-reported questionnaire for Rome IV criteria was completed by each participant. They also filled out a food frequency to assess their nutritional...

Abstract: Background: Hyperuricemia and inflammation are associated with the etiology pathogenesis of several metabolic diseases. Controlling elevated levels uric acid can be a potential therapeutic option for Aim: The current study examined anti-inflammatory anti-hyperuricemic properties Ficus benghalensis bark extract in vitro using murine macrophage RAW 246.7 cells. Materials Methods: Hydroalcoholic extracts were obtained. Cytotoxicity cell viability test was evaluated by MTT Trypan blue...

Background and Objectives: Folic acid (FA) is a necessary ingredient for numerous bodily activities including pregnancy. Because of this, women should have knowledge awareness the health benefits FA supplementation. Thus, we aimed to investigate level on importance determine associated factors among female college students at King Saud University in Riyadh, Saudi Arabia. Material Methods: We conducted cross-sectional study using questionnaire between January 2020 February 2021 aged 17 26...