We identified a potent NDM-1 inhibitor that formed S–Se bond with the Cys²²¹ residue at active site, thereby exhibiting new inhibition mechanism broad spectrum inhibitory potential.

The coronavirus outbreak that commenced at the end of 2019 has led to a dramatic increase in demand for face masks. In countries are experiencing shortage masks as result panic buying or inadequate supply, reusable fabric have become popular option, because they often considered more cost-effective and environmentally friendly than disposable medical Nevertheless, there remains significant variation quality performance existing masks; not all simultaneously able provide protection against...

Microplastics are recognised as a ubiquitous and hazardous pollutant worldwide. These small-sized particles have been detected in human faeces collected from number of cities, providing evidence ingestion microplastics their presence the gastrointestinal tract. Here, using Raman spectroscopy, we identified an average 50 g−1 (20.4–138.9 wet weight) healthy cohort Hong Kong. This quantity was about five times higher than values reported other places Asia Europe. Polystyrene most abundant...

Neuropsychiatric disorders have been one of the worldwide health problems contributing to profound social and economic consequences. It is reported that consumption an excessive high-fat diet (HFD) in middle age could induce cognitive emotional dysfunctions, whereas mechanisms effects long-term HFD intake on brain not fully investigated. We propose a hypothesis prolonged throughout adulthood lead neurobehavioral deterioration via gut-brain axis. In this study, adult C57BL/6J mice consuming...

Ribosome inactivating proteins (RIPs) like ricin, pokeweed antiviral protein (PAP) and Shiga-like toxins 1 2 (Stx1 Stx2) share the same substrate, alpha-sarcin/ricin loop, but differ in their specificities towards prokaryotic eukaryotic ribosomes. Ricin depurinates ribosomes more efficiently than ribosomes, while PAP can depurinate both types of Accumulating evidence suggests that different docking sites on ribosome might be used by RIPs, providing a basis for understanding mechanism...

Vibrio parahaemolyticus is commonly resistant to ampicillin, yet the mechanisms underlying this phenomenon are not clear. In study, a novel class A carbenicillin-hydrolyzing β-lactamase (CARB) family of β-lactamases, bla(CARB-17), was identified and found be responsible for intrinsic penicillin resistance in V. parahaemolyticus. Importantly, bla(CARB-17)-like genes were present all 293 genome sequences available GenBank detectable 91 food isolates, further confirming nature gene.

Food safety is one of the major concerns in every country regardless economic and social development. The frequent occurrence food scandals world has led Chinese government to implement several strategies fortify supply system a high standard. This relies heavily on laboratory testing services but conventional methods for detection contaminants toxicants are limited by sophisticated sample preparation procedures, long analysis time, large instruments professional personnel meet increasing...

The aim of this study was to investigate the structural characteristics and in vitro fermentation patterns polysaccharides from Boletus mushrooms. Polysaccharides were solubilized fruit bodies selected mushrooms auripes, B. bicolor, griseus using subcritical water extraction. characterized for their general physicochemical pattern, constituent monosaccharides molecular weight. A simulated model used utilization by gut microbiota consequent modulation microbial communities. Results showed...

Conceptual diagram of the current review covering synthesis, modification, properties, determination, and applications PHMB-based materials.

Recently, efforts at development of functional textiles with antibacterial effect have accelerated, the purpose being to provide protection against airborne bio-particles and micro-organisms. Growth microbes on surface textile materials can be inhibited by biocidal approach biostatic approach. This paper describes a healthcare durable properties optimizing conventional commercialized antimicrobial agent polybiguanide derivative—poly(hexamethylenebiguanide) (PHMB). Pad-dry-cure method was...

Ricin is a ribosome inactivating protein that catalytically removes universally conserved adenine from the alpha-sarcin/ricin loop (SRL) of 28S rRNA. We recently showed ricin A chain (RTA) interacts with P1 and P2 proteins ribosomal stalk to depurinate SRL in yeast. Here we examined interaction RTA wild-type mutant yeast ribosomes deleted by surface plasmon resonance. The between did not follow single-step binding model but was best characterized two distinct types interactions. AB1 had very...

ABSTRACT Carbapenems are one of the last lines defense for Gram-negative pathogens, such as members Enterobacteriaceae . Despite fact that most carbapenems resistant to extended-spectrum β-lactamase (ESBL), emerging metallo-β-lactamases (MBLs), including New Delhi metallo-β-lactamase 1 (NDM-1), can hydrolyze have become prevalent and frequently associated with so-called “superbugs,” which treatments extremely limited. Crystallographic study sheds light on modes antibiotic binding NDM-1, yet...

ABSTRACT A variety of CTX-M-type extended-spectrum β-lactamases (ESBLs), including hybrid ones, have been reported in China that are uncommon elsewhere. To better characterize the substrate profiles and enzymatic mechanisms these enzymes, we performed comparative kinetic analyses both parental CTX-M CTX-M-15, -132, -123, -64, -14 -55, known to confer variable levels β-lactam resistance host strains. All tested enzymes were susceptible serine β-lactamase inhibitors, with sulbactam exhibiting...

The role of the gut microbiota in various metabolic diseases has been widely studied. This study aims to test hypothesis that dysbiosis is associated with DOCA-salt-induced hypertension, while captopril, an antihypertensive drug, able rebalance alterations caused by hypertension. Treatment captopril resulted approximate 32 mmHg reduction systolic blood pressure (162.57 vs. 194.61 mmHg) hypertensive rats, although it was significantly higher than SHAM rats (136.10 mmHg). Moreover, nitric...

Toxicological research into the impact of plasticizer on different organs has been reported in past few decades, while their effects shifting gut microbiota and immune cells homeostasis zebrafish were only studied recently. However, studies human are scarce. In this study, we co-incubated healthy fecal with concentrations Di(2-ethylhexyl) phthalate (DEHP) di-iso-nonyl (DINP), analyzed microbial composition by 16S rDNA sequencing, compared influence derived microbiomes enterocyte (HT-29)...

Ricin produced by the castor bean plant and Shiga toxins pathogenic Escherichia coli (STEC) Shigella dysenteriae are type II ribosome inactivating proteins (RIPs), containing an enzymatically active A subunit that inhibits protein synthesis removing adenine from α-sarcin/ricin loop (SRL) of 28S rRNA. There currently no known antidotes to toxin or ricin, ability screen large chemical libraries for inhibitors has been hindered lack quantitative assays catalytic activity can be adapted a high...