A5009942851- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Angiogenesis and VEGF in Cancer
- Receptor Mechanisms and Signaling
- Cell Adhesion Molecules Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Proteoglycans and glycosaminoglycans research
- Neuropeptides and Animal Physiology
- Platelet Disorders and Treatments
- Respiratory and Cough-Related Research
- Peptidase Inhibition and Analysis
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Pediatric health and respiratory diseases
- Ion channel regulation and function
- TGF-β signaling in diseases
- Neuroscience of respiration and sleep
- Mast cells and histamine
- Inhalation and Respiratory Drug Delivery
- Thermal Regulation in Medicine
- Pharmacological Effects and Assays
- Cancer, Stress, Anesthesia, and Immune Response
- Drug Transport and Resistance Mechanisms
- Phosphodiesterase function and regulation
Rutgers, The State University of New Jersey
2016-2025
Johnson University
2022-2025
Rutgers Sexual and Reproductive Health and Rights
2017-2025
Rutgers Health
2025
University of Pennsylvania
2002-2018
South University
2009
Children's Hospital of Philadelphia
2005-2006
Philadelphia University
2003-2005
Thomas Jefferson University
1996-2000
Platelet endothelial cell adhesion molecule (PECAM)-1 has been implicated in angiogenesis, but a number of issues remain unsettled, including the independent involvement human PECAM-1 (huPECAM-1) tumor angiogenesis and mechanisms its participation vessel formation. We report for tumors grown skin transplanted on severe combined immunodeficiency mice that antibodies against huPECAM-1 (without simultaneous treatment with anti-VE-cadherin antibody) decreased density human, not murine, vessels...
Transforming growth factor β1 (TGF-β1), a cytokine whose levels are elevated in the airways of patients with asthma, perpetuates airway inflammation and modulates structural cell remodeling. However, role TGF-β1 excessive narrowing or hyperresponsiveness (AHR), remains unclear. In this study, we set out to investigate direct effects on human smooth muscle (HASM) shortening hyperresponsiveness. The dynamics AHR single-cell excitation-contraction coupling were measured precision-cut lung...
The final stage of lung development in humans and rodents occurs principally after birth involves the partitioning large primary saccules into smaller air spaces by inward protrusion septae derived from walls saccules. Several observations animal models implicate angiogenesis as critical to this process alveolarization, but all anti-angiogenic treatments examined date have resulted endothelial cell (EC) death. We therefore targeted function platelet adhesion molecule, (PECAM-1), an EC...
Several protocols for the isolation of endothelial cells (ECs) from murine lung have been described in literature. We, however, encountered a number problems while using these procedures that prevented us consistently or reliably obtaining pure populations ECs lungs mice. By incorporating specific elements previously published protocols, as well adding some novel features, we developed new strategy isolating lung. In this approach, suspension is initially prepared 7- to 14-day-old mouse pups...
The asthma-obesity syndrome represents a major public health concern that disproportionately contributes to asthma severity and induces insensitivity therapy. To date, no study has shown an intrinsic difference between human airway smooth muscle (HASM) cells derived from nonobese subjects those obese subjects. objective of this was address whether there is greater response agonist-induced calcium mobilization, phosphorylation myosin light chain (MLC), shortening in HASM were age sex matched....
Acute lung injury results in early inflammation and respiratory distress, later fibrosis. The glycosaminoglycan hyaluronan (HA) the Receptor for Hyaluronan-Mediated Motility (RHAMM, CD168) have been implicated response to acute injury. We hypothesized that, compared wild type (WT) mice, RHAMM knockout (KO) mice would be protected from, whereas with macrophage-specific transgenic overexpression of (TG) worse inflammation, distress fibrosis after intratracheal (IT) bleomycin. Compared WT 10...
Significance Stromal-interacting molecule 1 (STIM1) proteins are essential for the function of store-operated Ca 2+ entry (SOCE). Using transcriptomics, metabolomics, imaging, and inducible smooth muscle–specific STIM1 knockout mice expressing genetically encoded sensors, we reveal a crucial in airway remodeling hyperresponsiveness asthma. STIM1-mediated oscillations muscle (ASM) cells critical ASM through metabolic transcriptional reprogramming cytokine secretion, including IL-6. These...
The role of immune cells in neurodegeneration remains incompletely understood. Our recent study revealed the presence mucosal-associated invariant T (MAIT) meninges, where they express antioxidant molecules to maintain meningeal barrier integrity. Accumulation misfolded tau proteins are a hallmark neurodegenerative diseases. MAIT tau-related neuroinflammation and neurodegeneration, however, unclear. Here we report that meninges P301 mutant human transgenic mice had increased numbers cells,...
Idiopathic pulmonary fibrosis (IPF) is characterized by significant, but poorly understood immune and antibody responses. This study examines the spatial transcriptomes microenvironmental niches of antibody-producing plasma cells tertiary lymphoid structures (TLS) in IPF lungs, molecular pathways influencing accumulation fibrosis. Explant lung tissues from patients control normal lungs were used for transcriptome assays validating RNA-scope immunofluorescence assays. Fibroblasts derived...
The role of immune cells in neurodegeneration remains incompletely understood. Accumulation misfolded tau proteins is a hallmark neurodegenerative diseases. Our recent study revealed the presence mucosal-associated invariant T (MAIT) meninges, where they express antioxidant molecules to maintain meningeal barrier integrity. However, MAIT tau-related neuroinflammation and unknown. Flow cytometry analysis was performed examine human Tau P301S transgenic mice. pathology, hippocampus atrophy,...
Most patients who die from cancer succumb to treatment-refractory advanced metastatic progression. Although the early stages of tumor metastasis result in formation clinically silent micrometastatic foci, its later primarily reflect progressive, organ-destructive growth already metastases. Early-stage is regulated by multiple factors within cells as well microenvironment (TME). In contrast, molecular determinants that control progression remain essentially uncharacterized, precluding...
Background and Purpose Asthma manifests as a heterogeneous syndrome characterized by airway obstruction, inflammation hyperresponsiveness (AHR). Although the molecular mechanisms remain unclear, activation of specific PI3K isoforms mediate AHR. We aimed to determine whether inhibition PI3Kδ evokes dilation airways elucidate potential mechanisms. Experimental Approach Human precision cut lung slices from non‐asthma donors primary human smooth muscle (HASM) cells both asthma were utilized....
Asthma is one of the most common chronic diseases globally and can be divided into presenting with or without an immune response. Current therapies have little effect on nonimmune disease, mechanisms that drive this type asthma are poorly understood. Here, we shown loss transcription factors forkhead box P1 (Foxp1) Foxp4, which critical for lung epithelial development, in adult airway epithelium evokes a non-Th2 phenotype characterized by hyperresponsiveness (AHR) eosinophilic inflammation....
Leptin is the protein product of recently cloned obesity gene. receptor mRNA found in a number central and peripheral locations. The hypothalamus presumed site action. However, little known about specific locations organs. Epinephrine has potent anorectic effects can cause weight loss by variety mechanisms. Excretion epinephrine reduced ob/ob mouse, which lacks leptin, suggesting an effect leptin on adrenal medulla. In current study, presence was identified epinephrine-secreting cells...
Platelet endothelial cell adhesion molecule (PECAM-1), a transmembrane glycoprotein, has been implicated in angiogenesis, with recent evidence indicating the involvement of PECAM-1 motility. The cytoplasmic domain contains two tyrosine residues, Y663 and Y686, that each fall within an immunoreceptor tyrosine-based inhibitory motif (ITIM). When phosphorylated, these residues together mediate binding protein phosphatase SHP-2. Because SHP-2 shown to be involved turnover focal adhesions,...
PI3K-dependent activation of Rho kinase (ROCK) is necessary for agonist-induced human airway smooth muscle cell (HASMC) contraction, and inhibition PI3K promotes bronchodilation small airways. The mechanisms driving agonist-mediated PI3K/ROCK axis activation, however, remain unclear. Given that G