A5030809755- Cardiovascular Function and Risk Factors
- Heart Failure Treatment and Management
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cardiac Valve Diseases and Treatments
- Respiratory Support and Mechanisms
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- Mass Spectrometry Techniques and Applications
- COVID-19 and healthcare impacts
- PI3K/AKT/mTOR signaling in cancer
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Protein Kinase Regulation and GTPase Signaling
- Cardiac Arrest and Resuscitation
- Cardiac Imaging and Diagnostics
- TGF-β signaling in diseases
- Bioinformatics and Genomic Networks
- Bacterial Genetics and Biotechnology
- Pharmacological Effects and Assays
- Science, Research, and Medicine
- Pulmonary Hypertension Research and Treatments
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
Icahn School of Medicine at Mount Sinai
2020-2025
Johns Hopkins Medicine
2022-2025
Rutgers, The State University of New Jersey
2016-2025
Johns Hopkins University
2022-2025
California University of Pennsylvania
2025
Albert Einstein College of Medicine
2022-2023
Mount Sinai Hospital
2021
Translational Therapeutics (United States)
2017
Rutgers Sexual and Reproductive Health and Rights
2017
University of Pennsylvania
2017
The human heart primarily metabolizes fatty acids, and this decreases as alternative fuel use rises in failure with reduced ejection fraction (HFrEF). Patients severe obesity diabetes are thought to have increased myocardial acid metabolism, but whether is found those who also preserved (HFpEF) unknown.
Transforming growth factor β1 (TGF-β1), a cytokine whose levels are elevated in the airways of patients with asthma, perpetuates airway inflammation and modulates structural cell remodeling. However, role TGF-β1 excessive narrowing or hyperresponsiveness (AHR), remains unclear. In this study, we set out to investigate direct effects on human smooth muscle (HASM) shortening hyperresponsiveness. The dynamics AHR single-cell excitation-contraction coupling were measured precision-cut lung...
Background and Purpose Asthma manifests as a heterogeneous syndrome characterized by airway obstruction, inflammation hyperresponsiveness (AHR). Although the molecular mechanisms remain unclear, activation of specific PI3K isoforms mediate AHR. We aimed to determine whether inhibition PI3Kδ evokes dilation airways elucidate potential mechanisms. Experimental Approach Human precision cut lung slices from non‐asthma donors primary human smooth muscle (HASM) cells both asthma were utilized....
Asthma is one of the most common chronic diseases globally and can be divided into presenting with or without an immune response. Current therapies have little effect on nonimmune disease, mechanisms that drive this type asthma are poorly understood. Here, we shown loss transcription factors forkhead box P1 (Foxp1) Foxp4, which critical for lung epithelial development, in adult airway epithelium evokes a non-Th2 phenotype characterized by hyperresponsiveness (AHR) eosinophilic inflammation....
Background Heart failure with preserved ejection fraction (HFpEF) constitutes more than half of all HF but has few effective therapies. Recent human myocardial transcriptomics and metabolomics have identified major differences between HFpEF controls. How this translates at the protein level is unknown. Methods Results Myocardial tissue from patients nonfailing donor controls was analyzed by data‐dependent acquisition (n=10 HFpEF, n=10 controls) data‐independent (n=44 n=5 mass...
The objective of this study is to describe the prevalence inflammatory cardiopulmonary findings in a prospective cohort long coronavirus disease (LC) patients. Methods: Subjects with history 2019 infection, persistent symptoms 9-12 mo after initial and clinical assessment compatible LC underwent 18F-FDG PET/MRI, dual-energy CT (DECT) lungs, plasma protein analysis (subgroup). A control group that included subjects acute severe respiratory syndrome 2 infection but without at recruitment was...
PI3K-dependent activation of Rho kinase (ROCK) is necessary for agonist-induced human airway smooth muscle cell (HASMC) contraction, and inhibition PI3K promotes bronchodilation small airways. The mechanisms driving agonist-mediated PI3K/ROCK axis activation, however, remain unclear. Given that G
Abstract The analysis of exhaled breath condensate (EBC) demonstrates a promising avenue minimally invasive biopsies for diagnostics. EBC is obtained by cooling air and collecting the condensation to be utilized downstream using various analytical methods. aqueous phase contains large variety miscible small compounds including polar electrolytes, amino acids, cytokines, chemokines, peptides, proteins, metabolites, nucleic lipids/eicosanoids—however, these analytes are typically present at...
Formaldehyde, a common indoor air pollutant, exacerbates asthma and synergizes with allergen to induce airway hyperresponsiveness (AHR) in animal models. The mechanisms mediating formaldehyde-induced AHR remain poorly understood. We posit that formaldehyde modulates agonist-induced contractile response of human smooth muscle (HASM) cells elicit AHR. HASM were exposed or vehicle intracellular Ca2+ ([Ca2+]i) myosin light-chain phosphatase (MYPT1) phosphorylation determined. Air-liquid...
Acute hypoxemic respiratory failure is the major complication of coronavirus disease 2019, yet optimal support strategies are uncertain. We aimed to describe outcomes with high-flow oxygen delivered through nasal cannula and noninvasive positive pressure ventilation in 2019 acute identify individual factors associated failure.Retrospective cohort study rates and/or success (live discharge without endotracheal intubation). Fine-Gray subdistribution hazard models were used patient...
Flat cultures of mammalian cells are a widely used in vitro approach for understanding cell physiology, but this system is limited modeling solid tissues due to unnaturally rapid replication. This particularly challenging when mature chromatin, as fast replicating frequently involved DNA replication and have heterogeneous polyploid population. Presented below workflow modeling, treating, analyzing quiescent chromatin modifications using three-dimensional (3D) culture system. Using protocol,...
Abstract Objectives Mortality risk scores, such as SOFA, qSOFA, and CURB-65, are quick, effective tools for communicating a patient’s prognosis guiding therapeutic decisions. Most use simple calculations that can be performed by hand. While several COVID-19 specific scores exist, they lack the ease of these simpler scores. The objectives this study were (1) to design, validate, calibrate simple, easy-to-use mortality score patients (2) recalibrate CURB-65 in hospitalized population. Design...
Flat cultures of mammalian cells are a widely used in vitro approach for understanding cell physiology, but this system is limited modeling solid tissues due to unnaturally rapid replication. This particularly challenging when mature chromatin, as fast replicating frequently involved DNA replication and have heterogeneous polyploid population. Presented below workflow modeling, treating, analyzing quiescent chromatin modifications using three-dimensional (3D) culture system. Using protocol,...
ABSTRACT Rationale Acute hypoxemic respiratory failure (AHRF) is the major complication of coronavirus disease 2019 (COVID-19), yet optimal support strategies are uncertain. Objectives To describe outcomes with high-flow oxygen delivered through nasal cannula (HFNC) and non-invasive positive pressure ventilation (NIPPV) in COVID-19 AHRF identify individual factors associated failure. Methods We performed a retrospective cohort study hospitalized adults treated HFNC and/or NIPPV to rates...