A5091775848- Glioma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Myeloid Leukemia Research
- Brain Metastases and Treatment
- T-cell and B-cell Immunology
- RNA modifications and cancer
- Nuclear Receptors and Signaling
- Alzheimer's disease research and treatments
- Protein Degradation and Inhibitors
- Genomics and Phylogenetic Studies
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Genetics, Aging, and Longevity in Model Organisms
- Viral Infections and Outbreaks Research
- Immune cells in cancer
- COVID-19 epidemiological studies
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Plant Disease Resistance and Genetics
- Muscle Physiology and Disorders
Mayo Clinic in Florida
2019-2024
WinnMed
2019-2024
Jacksonville College
2019-2021
HudsonAlpha Institute for Biotechnology
2018
Indiana University – Purdue University Indianapolis
2016-2017
Walker (United States)
2016
Abstract Sugarcane ( Saccharum spp.) is a major crop for sugar and bioenergy production. Its highly polyploid, aneuploid, heterozygous, interspecific genome poses challenges producing reference sequence. We exploited colinearity with sorghum to produce BAC-based monoploid sequence of sugarcane. A minimum tiling path 4660 sugarcane BAC that best covers the gene-rich part was selected based on whole-genome profiling, sequenced, assembled in 382-Mb single high-quality total 25,316...
Abstract APOE4 is the strongest genetic risk factor associated with late-onset Alzheimer’s disease (AD). To address underlying mechanism, we develop cerebral organoid models using induced pluripotent stem cells (iPSCs) APOE ε3/ε3 or ε4/ε4 genotype from individuals either normal cognition AD dementia. Cerebral organoids patients carrying show greater apoptosis and decreased synaptic integrity. While patient-derived have increased levels of Aβ phosphorylated tau compared to healthy...
Tumors acquire numerous mutations during development and progression. When translated into proteins, these give rise to neoantigens that can be recognized by T cells generate antibodies, representing an exciting direction of cancer immunotherapy. While have been reported in many types, the profiling often focused on class-I subtype are presented CD8 + cells, relationship between neoantigen load clinical outcomes was inconsistent among types. In this study, we described informatics workflow,...
With technological advancements, human’s desire to explore space is growing and more people are staying longer at the international station (ISS). The impact of microgravity on stem cells (SC) not fully understood. We explored gene expression profile cultured mesenchymal stem/stromal (MSCs) ISS. also evaluated how new knowledge gained sheds light our understanding human physiology Earth. Primary cultures MSCs were expanded ISS for 1 or 2 weeks mRNA was isolated from samples cells. Gene...
Improved DNA sequencing methods have transformed the field of genomics over last decade. This has become possible due to development inexpensive short read technologies which now resulted in three generations platforms. More recently, a new fourth generation Nanopore based single molecule technology, was developed on MinION(®) sequencer is portable, and fast. It capable generating reads length greater than 100 kb. Though it many specific advantages, two major limitations MinION are high...
Abstract IL-2 is a pleiotropic cytokine that promotes the differentiation of Th cell subsets, including Th1, Th2, and Th9 cells, but it impairs development Th17 T follicular helper cells. Although produced by all polarized subsets to some level, how impacts production when effector cells are restimulated unknown. We show in this article Golgi transport inhibitors (GTIs) blocked IL-9 production. Mechanistically, GTIs secretion normally feeds back paracrine manner promote STAT5 activation...
Bcl-2 and Mcl-1 proteins play a role in multiple myeloma (MM) cell survival, for which targeted inhibitors are being developed. AT-101 is an oral drug, disrupts function, impedes mitochondrial bioenergetic processes induces apoptosis MM cells. When combined with lenalidomide dexamethasone (Rd), significantly reduced tumor burden vivo xenograft model of MM. These data provided rationale phase I/II study to establish the effective dose combination Rd (ARd regimen) relapsed/refractory A total...
INTRODUCTION: Glioblastoma (GBM) is the most common and devastating primary brain tumor in adults. The current standard of care includes maximal safe resection followed by concurrent radiation temozolomide-based chemotherapy. However, preoperative radiotherapy used different cancer types commonly for metastasis to shrink tumors, but it has never been GBM. METHODS: Thirty-nine immunocompromised adult athymic nude female rats were orthotopically engrafted with a GBM1A patient-derived glioma...
Abstract Background Systemic inflammation plays a pivotal role in many chronic diseases including Alzheimer’s disease (AD). Assessing the composition of immune pathways neurodegenerative can contribute to precision medicine. Using publicly available transcriptomic data, we sought elucidate transcriptional networks pertinent inflammatory across brain regions and peripheral blood AD/mild cognitive impairment (MCI) Parkinson’s (PD). Method For AD/MCI vs. control dataset, analyzed bulk‐RNAseq...
We analyzed over 22,000 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes of patient samples tested at Mayo Clinic Laboratories during a 2-year period in the COVID-19 pandemic, which included Alpha, Delta, and Omicron variants concern to examine roles relationships Minnesota virus transmission. found that Hennepin County, most populous county, drove transmission SARS-CoV-2 viruses state after including formation earlier clades 20A, 20C, 20G, as well Alpha Delta. also...
Abstract Glioblastoma (GBM) is the most devastating and common form of primary brain cancer in adults. Tumor location plays a significant role patient prognosis; particular, GBM tumors contacting lateral ventricles (LVs) are more aggressive than LV-distal counterparts. This may be due to interaction with unique features LV microenvironment, including cerebrospinal fluid (CSF) neural progenitor cells (NPCs) subventricular zone. Despite contact having on tumor malignancy, studies using bulk...
Abstract Background Alzheimer’s disease (AD) is a complex, multicellular with immunological and genetic components. Previous studies demonstrated associations between AD the missense variants ABI3‐rs616338‐T PLCG2‐rs72824905‐G. The perturbed expression of these genes has been to disrupt microglial function. To date, transcriptomic profiles variant carriers in context subtype state have not explored human brain. This represents knowledge gap our understanding risk resilience. We developed...
7021 Background: Case-control studies (CCS) have identified germline variants (GVs) & genes associated with AML risk and/or outcome. However, whether these also harbor somatic mutations (SMs) in and SMs are clinical phenotypes outcome is unknown. We systemically interrogated PubMed (terms "AML genetic polymorphisms"), yielding 612 reports (1981-2018), from which we unique variants/single nucleotide polymorphisms (SNPs) 100 CCS. To determine the prevalence relevance of an unselected...
The ε4 allele of the apolipoprotein E gene (APOE4) is strongest genetic risk factor for late-onset Alzheimer’s disease (AD) compared to common ε3 allele. Because APOE4 also associated with an increased several vascular conditions including small vessel and cognitive impairment (VCI), apoE4 effects on cerebrovasculature might be a converging pathway impacting brain homeostasis. As apoE abundantly expressed in multiple cell types astrocytes, microglia, mural cells (VMCs), defining vivo effect...
SARS-CoV-2 has had an unprecedented impact on human health and highlights the need for genomic epidemiology studies to increase our understanding of virus evolution spread, inform policy decisions. We sequenced viral genomes from over 22,000 patient samples tested at Mayo Clinic Laboratories between 2020-2022 use Bayesian phylodynamics describe county regional spread in Minnesota. The earliest introduction into Minnesota was Hennepin County a domestic source around January 22, 2020; six...