A5067781788- Sphingolipid Metabolism and Signaling
- Angiogenesis and VEGF in Cancer
- Proteoglycans and glycosaminoglycans research
- Calcium signaling and nucleotide metabolism
- Vitamin D Research Studies
- Lipid Membrane Structure and Behavior
- Paraquat toxicity studies and treatments
- Fibroblast Growth Factor Research
- S100 Proteins and Annexins
- Genomics, phytochemicals, and oxidative stress
- Skin Protection and Aging
- Peptidase Inhibition and Analysis
- Neurogenesis and neuroplasticity mechanisms
- HER2/EGFR in Cancer Research
- Lysosomal Storage Disorders Research
- Gastrointestinal Bleeding Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Heavy Metal Exposure and Toxicity
- Arsenic contamination and mitigation
- Immunodeficiency and Autoimmune Disorders
- Endoplasmic Reticulum Stress and Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nerve injury and regeneration
- Flame retardant materials and properties
- Liver Disease Diagnosis and Treatment
Chung-Ang University
2012-2024
Xihua University
2022
Chinese Center For Disease Control and Prevention
2019-2020
Paraquat dichloride (N,N-dimethyl-4-4'-bipiridinium, PQ) is an extremely toxic chemical that widely used in herbicides. PQ generates reactive oxygen species (ROS) and causes multiple organ failure. In particular, has been reported to be immunotoxic agrochemical compound. was shown decrease the number of macrophages rats suppress monocyte phagocytic activity mice. However, effect on macrophage cell viability remains unclear. this study, we evaluated cytotoxic mouse line, RAW264.7 its possible...
Autophagy is essential for optimal cell function and survival, the entire process accompanies membrane dynamics. Ceramides are produced by different enzymes at cellular sites mediate differential signaling. However, it remains unclear which ceramide-producing pathways/enzymes participate in autophagy regulation under physiological conditions such as nutrient starvation, what underlying mechanisms are. In this study, we demonstrate that among enzymes, neutral sphingomyelinase 2 (nSMase2)...
Abstract Major depressive disorder (MDD) is the most common nonfatal disease burden worldwide. Systemic chronic low-grade inflammation has been reported to be associated with MDD progression by affecting monoaminergic and glutamatergic neurotransmission. However, whether various proinflammatory cytokines are abnormally elevated before first episode of depression still largely unclear. Here, we evaluated 184 adolescent patients who were experiencing their disorder, same number healthy...
The skin seems to rejuvenate upon exposure factors within the circulation of young organisms. Intrinsic that modulate aging are poorly understood. We used heterochronic parabiosis and aptamer-based proteomics identify serum-derived rejuvenating factors. discovered a novel extracellular function hyaluronan proteoglycan link protein 1 (HAPLN1). Its serum levels decreased with age, disturbing integrity matrix, which is predominantly composed collagen I hyaluronan; various markers, decrease in...
Paraquat (PQ), one of the most widely used herbicides, has been for several decades in agriculture. Some studies suggest that PQ effects on immune system. Moreover, previous have shown imparted some immunosuppressive effects. In present study, cytotoxicity assays using splenic NK cells from mice treated 28 days with (at 0.2, 1, and 5 mg/kg) were performed to determine whether altered function cells. Given was expected induce an effect, it hypothesized a gene involved cellular metal ion...
Other SectionsABSTRACTINTRODUCTIONRESULTSrhHAPLN1 attenuates PDGF-BB-induced de-differentiation of HASMCs and plays an important role in phenotypic switching to the contractile stateDISCUSSIONMATERIALS AND METHODSACKNOWLEDGMENTSCONFLICTS OF INTERESTFIGURESREFERENCES
Chronic obstructive pulmonary disease (COPD) will be the third leading cause of death worldwide by 2030.One its components, emphysema, has been defined as a lung that irreversibly damages lungs' alveoli.Treatment is currently unavailable for emphysema symptoms and complete cure disease.Hyaluronan (HA) proteoglycan link protein 1 (HAPLN1), an HA-binding linking HA in extracellular matrix to stabilize structure, forms bulky hydrogel-like aggregate.Studies on biological role full-length HAPLN1,...
Cardiovascular diseases (CVDs) are the most common cardiovascular system disorders.Cellular senescence is a key mechanism associated with dysfunction of aged vascular endothelium.Hyaluronic acid and proteoglycan link protein 1 (HAPLN1) has been known to non-covalently hyaluronic (HA) proteoglycans (PGs), forms stabilizes HAPLN1-containing aggregates as major component extracellular matrix.Our previous study showed that serum levels HAPLN1 decrease aging.Here, we found gene expression was...
Activation of sphingomyelinase (SMase) by extracellular stimuli is the major pathway for cellular production ceramide, a bioactive lipid mediator acting through sphingomyelin (SM) hydrolysis. Previously, we reported existence six forms neutral pH–optimum and Mg2+-dependent SMase (N-SMase) in membrane fractions bovine brain. Here, focus on N-SMase ε from salt-extracted membranes. After extensive purification 12,780-fold with yield 1.3%, this enzyme was eventually characterized as N-SMase2....
Nerve growth factor (NGF)-induced neuronal regeneration has emerged as a strategy to treat degeneration-associated disorders. However, direct NGF administration is limited by the occurrence of adverse effects at high doses NGF. Therefore, development therapeutic promote trophic effect required. In view lack understanding mechanism for potentiating effect, this study investigated molecular targets DA-9801, well-standardized Dioscorea rhizome extract, which promoting on An increase in...
Ceramide metabolism is known to be an essential etiology for various diseases, such as atopic dermatitis and Gaucher disease. Glucosylceramide synthase (GCS) a key enzyme the synthesis of glucosylceramide (GlcCer), which main ceramide pathway in mammalian cells. In this article, we developed liquid chromatography-tandem mass spectrometry (LCMS/MS) method determine GCS activity using synthetic non-natural sphingolipid C8-ceramide substrate. The reaction products, C8-GlcCer GCS, could...
Sphingomyelinase (SMase) catalyzes ceramide production from sphingomyelin. Ceramides are critical in cellular responses such as apoptosis. They enhance mitochondrial outer membrane permeabilization (MOMP) through self-assembly the to form channels that release cytochrome c intermembrane space (IMS) into cytosol, triggering caspase-9 activation. However, SMase involved MOMP is yet be identified. Here, we identified a Mg
Hair loss is a common condition that can have negative impact on an individual's quality of life.The severe side effects and the low efficacy current hair medications create unmet needs in field treatment.Hyaluronan Proteoglycan Link Protein 1 (HAPLN1), one components extracellular matrix, has been shown to play role maintaining its integrity.HAPLN1 was examined for ability growth with less than existing treatments.HAPLN1 predominantly expressed anagen phase three stages cycle mice promotes...
Objective To explore the effects and mechanism of neuregulin-1 (NRG-1) in rat cardiac microvascular endothelial cells (CMECs)-cardiomyocytes coculture, during ischemia/reperfusion injury. Methods Cardiac myocytes were isolated from hearts neonatal rats CMECs adult rats. CMECs-cardiomyocytes coculture model was established. Then endured simulated I/R(4 h/4 h). Collected culture medium SI/R. The cell viability cardiomyocytes measured by MTT assay. apoptosis detected TUNEL method....
Yongwei Piao, So Yoon Yun, Hee Soo Kim, Bo Kyung Park, Hae Chan Ha, Zhicheng Fu, Ji Min Jang, Moon Jung Back, In Chul Shin, Jong Hoon Won and Dae Kyong Kim. Biomolecules & Therapeutics 2022;30:529-39. https://doi.org/10.4062/biomolther.2022.071
Fire safety propaganda and education is crucial for protecting the public their property.However, popularization of science regarding fire-and flame-retardant-related knowledge, as an important strategy to improve national awareness fire reduce occurrence accidents, currently limited.This paper presents a simple method fabricating "expandable" flame retardant fabric coatings.Chemical especially three aspects combustion, materials, mechanisms, popular among young people owing public's...