Paraquat dichloride (N,N-dimethyl-4-4'-bipiridinium, PQ) is an extremely toxic chemical that widely used in herbicides. PQ generates reactive oxygen species (ROS) and causes multiple organ failure. In particular, has been reported to be immunotoxic agrochemical compound. was shown decrease the number of macrophages rats suppress monocyte phagocytic activity mice. However, effect on macrophage cell viability remains unclear. this study, we evaluated cytotoxic mouse line, RAW264.7 its possible...

Autophagy is essential for optimal cell function and survival, the entire process accompanies membrane dynamics. Ceramides are produced by different enzymes at cellular sites mediate differential signaling. However, it remains unclear which ceramide-producing pathways/enzymes participate in autophagy regulation under physiological conditions such as nutrient starvation, what underlying mechanisms are. In this study, we demonstrate that among enzymes, neutral sphingomyelinase 2 (nSMase2)...

In the present study, we examined mechanisms underlying effect of DA-9801 on neurite outgrowth. We found that elicits its effects via mitogen-activated protein kinase (MEK) extracellular signal-regulated (ERK)1/2-cAMP response element-binding (CREB) pathway. DA-9801, an extract from a mixture Dioscorea japonica and nipponica, was reported to promote outgrowth in PC12 cells. The cell viability expression neuronal markers were evaluated To investigate action, specific inhibitors targeting ERK...

The skin seems to rejuvenate upon exposure factors within the circulation of young organisms. Intrinsic that modulate aging are poorly understood. We used heterochronic parabiosis and aptamer-based proteomics identify serum-derived rejuvenating factors. discovered a novel extracellular function hyaluronan proteoglycan link protein 1 (HAPLN1). Its serum levels decreased with age, disturbing integrity matrix, which is predominantly composed collagen I hyaluronan; various markers, decrease in...

Paraquat (PQ), one of the most widely used herbicides, has been for several decades in agriculture. Some studies suggest that PQ effects on immune system. Moreover, previous have shown imparted some immunosuppressive effects. In present study, cytotoxicity assays using splenic NK cells from mice treated 28 days with (at 0.2, 1, and 5 mg/kg) were performed to determine whether altered function cells. Given was expected induce an effect, it hypothesized a gene involved cellular metal ion...

Other SectionsABSTRACTINTRODUCTIONRESULTSrhHAPLN1 attenuates PDGF-BB-induced de-differentiation of HASMCs and plays an important role in phenotypic switching to the contractile stateDISCUSSIONMATERIALS AND METHODSACKNOWLEDGMENTSCONFLICTS OF INTERESTFIGURESREFERENCES

Chronic obstructive pulmonary disease (COPD) will be the third leading cause of death worldwide by 2030.One its components, emphysema, has been defined as a lung that irreversibly damages lungs' alveoli.Treatment is currently unavailable for emphysema symptoms and complete cure disease.Hyaluronan (HA) proteoglycan link protein 1 (HAPLN1), an HA-binding linking HA in extracellular matrix to stabilize structure, forms bulky hydrogel-like aggregate.Studies on biological role full-length HAPLN1,...

Cardiovascular diseases (CVDs) are the most common cardiovascular system disorders.Cellular senescence is a key mechanism associated with dysfunction of aged vascular endothelium.Hyaluronic acid and proteoglycan link protein 1 (HAPLN1) has been known to non-covalently hyaluronic (HA) proteoglycans (PGs), forms stabilizes HAPLN1-containing aggregates as major component extracellular matrix.Our previous study showed that serum levels HAPLN1 decrease aging.Here, we found gene expression was...

Background/Aim: Interleukin (IL)-1β is a pro-inflammatory cytokine that has recently been established as stimulator of angiogenesis via regulation proangiogenic factor expression in the tumor microenvironment. This study aimed to demonstrate inhibitory effects Robinia pseudoacacia leaf extract (RP) on IL-1β–mediated angiogenesis. Materials and Methods: Secreted embryonic alkaline phosphatase (SEAP) reporter gene assay, ex vivo vitro tube formation western blot, quantitative PCR were used...

This study embarks on a detailed cohort analysis to identify the factors influencing life-sustaining treatment (LST) decisions and healthcare usage before death. Utilizing multinomial logistic regression, it examines diverse sample of 1,072 patients, categorized into three groups: self-determination, family determination, DNR. The majority cohort, 49.4%, falls under self-determination group, with significant proportion patients (92.8%) suffering from cancer. We find that most common medical...

Ceramide metabolism is known to be an essential etiology for various diseases, such as atopic dermatitis and Gaucher disease. Glucosylceramide synthase (GCS) a key enzyme the synthesis of glucosylceramide (GlcCer), which main ceramide pathway in mammalian cells. In this article, we developed liquid chromatography-tandem mass spectrometry (LCMS/MS) method determine GCS activity using synthetic non-natural sphingolipid C8-ceramide substrate. The reaction products, C8-GlcCer GCS, could...

Sphingomyelinase (SMase) catalyzes ceramide production from sphingomyelin. Ceramides are critical in cellular responses such as apoptosis. They enhance mitochondrial outer membrane permeabilization (MOMP) through self-assembly the to form channels that release cytochrome c intermembrane space (IMS) into cytosol, triggering caspase-9 activation. However, SMase involved MOMP is yet be identified. Here, we identified a Mg

Hair loss is a common condition that can have negative impact on an individual's quality of life.The severe side effects and the low efficacy current hair medications create unmet needs in field treatment.Hyaluronan Proteoglycan Link Protein 1 (HAPLN1), one components extracellular matrix, has been shown to play role maintaining its integrity.HAPLN1 was examined for ability growth with less than existing treatments.HAPLN1 predominantly expressed anagen phase three stages cycle mice promotes...

Histiocytic necrotizing lymphadenitis, which is also commonly referred to as Kikuchi's disease (KD), a self-limiting of unknown etiology. It affects individuals all ages, although it usually seen in young women. However, only few descriptions this are available the pediatric literature. KD clinically characterized by cervical lymphadenopathy, high fever, myalgia, neutropenia and, rarely, cutaneous eruptions. Cutaneous manifestations have been reported 16-40 percent cases. The specific skin...

A 3-year-old girl presented with polydipsia, polyuria, hyponatremia, hypertension and congestive heart failure. Her polyuria was unresponsive to water restriction vasopressin challenge tests, her blood pressure not effectively controlled by antihypertensive drugs. Radiologic examinations revealed a Wilms' tumor in the right kidney. plasma renin activity aldosterone concentration were greatly increased. After surgical removal of tumor, failure disappeared. Congestive due is very rare we...

The typical radiographic findings of pulmonary edema from the increased hydrostatic pressure shows centrally localized consolidation, which is known as a butterfly or bat's wing pattern. These terms describe anatomic distribution that uniformly involve hilum and medulla lung but not peripheral region parenchyma (cortex). We present case wing-like on chest radiograph by mitral regurgitation due to an idiopathic chordal rupture.

Yongwei Piao, So Yoon Yun, Hee Soo Kim, Bo Kyung Park, Hae Chan Ha, Zhicheng Fu, Ji Min Jang, Moon Jung Back, In Chul Shin, Jong Hoon Won and Dae Kyong Kim. Biomolecules & Therapeutics 2022;30:529-39. https://doi.org/10.4062/biomolther.2022.071