A5001434719- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Sulfur Compounds in Biology
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Biomedical Research and Pathophysiology
- Skin Protection and Aging
- Endoplasmic Reticulum Stress and Disease
- Tendon Structure and Treatment
- Platelet Disorders and Treatments
- Lipid metabolism and disorders
- Skin and Cellular Biology Research
- Adipokines, Inflammation, and Metabolic Diseases
- Rheology and Fluid Dynamics Studies
- Osteoarthritis Treatment and Mechanisms
- RNA Interference and Gene Delivery
- Sphingolipid Metabolism and Signaling
- Redox biology and oxidative stress
- Psoriasis: Treatment and Pathogenesis
- Bladder and Urothelial Cancer Treatments
- Hematopoietic Stem Cell Transplantation
- Hibiscus Plant Research Studies
- Wound Healing and Treatments
- Cell death mechanisms and regulation
- Fibroblast Growth Factor Research
Jeju National University
2020-2024
Seoul National University
2010-2020
Seoul National University Hospital
2016
Interface (United Kingdom)
2015
Chung-Ang University
2011
Pulmonary and Critical Care Associates
2011
The core functions of stem cells (SCs) are critically regulated by their cellular redox status. Glutathione is the most abundant non-protein thiol functioning as an antioxidant and a regulator. However, investigation into relationship between glutathione-mediated capacity SC activities hindered lack probe. Here, we demonstrate that cyanoacrylamide-based coumarin derivatives ratiometric probes suitable for real-time monitoring glutathione levels in living SCs. These revealed heterogeneous...
Aberrant glutathione or Ca(2+) homeostasis due to oxidative stress is associated with the pathogenesis of neurodegenerative disorders. The Ca(2+)-permeable transient receptor potential cation (TRPC) channel predominantly expressed in brain, which sensitive stress. However, role TRPC neurodegeneration not known. Here, we report a mechanism TRPC5 activation by oxidants and effect glutathionylated on striatal neurons Huntington's disease. Intracellular oxidized leads via S-glutathionylation at...
Pulmonary fibrosis is a potentially life-threatening disease that may be caused by overt or asymptomatic inflammatory responses. However, the precise mechanisms which tissue injury translated into inflammation and consequent remain to established. Here, we show in lung model, bleomycin induced secretion of IL-6 epithelial cells transglutaminase 2 (TG2)–dependent manner. This response represents key step differentiation IL-17–producing T subsequent amplification lung. The essential role...
Highlights•Sirt1 regulates the expression of imprinted and germline-specific genes•Sirt1 restricts DNA methylation in antagonizes Dnmt3l at level transcription protein stability•Sirt1 deficiency impairs differentiation potency ESCs dependent on Dnmt3lSummaryEmbryonic stem cell (ESC) abnormalities genome hamper utility their therapeutic derivatives; however, underlying mechanisms are unknown. Here, we show that nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, Sirt1, selectively...
Transglutaminase2 (TG2) is a multi-functional protein involved in various cellular processes, including apoptosis, differentiation, wound healing, and angiogenesis. The malfunction of TG2 causes many human disease inflammatory disease, celiac neurodegenerative diseases, tissue fibrosis, cancers. Protein cross-linking activity, which representative TG2, activated by calcium ions suppressed GTP. Here, we elucidated the structure complex with its endogenous inhibitor, Our showed why GTP optimal...
Real-time monitoring of GSH dynamics demonstrates the functional roles CREB1-NRF2 pathway in MSC therapeutic potency.
Transglutaminase 2 (TGase2) is a ubiquitously expressed enzyme that catalyzes irreversible post-translational modification of protein, forming cross-linked protein aggregates. We previously reported intracellular TGase2 activated by oxidative stress. To elucidate the functional role activation in cells under oxidatively stressed condition, we identified mediator activates TGase2. In this study, showed low levels stress trigger release TGFbeta, which subsequently through nuclear translocation...
An abrupt increase of intracellular Ca2+ is observed in cells under hypoxic or oxidatively stressed conditions. The dysregulated cytosolic triggers apoptotic cell death through mitochondrial swelling and activation Ca2+-dependent enzymes. Transglutaminase 2 (TG2) a enzyme that catalyzes transamidation reaction producing cross-linked polyaminated proteins. TG2 activity known to be involved the process. However, pro-apoptotic role still controversial. In this study, we investigate apoptosis...
Tumor-promoting inflammation is a hallmark of cancer and highly associated with tumor progression, angiogenesis, metastasis. Tumor-associated macrophages (TAMs) are major drivers tumor-promoting inflammation, but due to the complexity microenvironment, detailed regulatory mechanisms still under investigation. Here, we investigated novel role for transglutaminase 2 (TGM2) in development recruitment TAMs gastric (GC) tissues. When estimated by array comparative genomic hybridization droplet...
Abstract Aberrant activation of embryogenesis-related molecular programs in urothelial bladder cancer (BC) is associated with stemness features related to oncogenic dedifferentiation and tumor metastasis. Recently, we reported that overexpression transcription factor CP2-like protein-1 (TFCP2L1) its phosphorylation at Thr177 by cyclin-dependent kinase-1 (CDK1) play key roles regulating carcinogenesis. However, the clinical relevance therapeutic potential this novel CDK1-TFCP2L1 network...
Abstract UV irradiation elicits acute inflammation in the skin by increasing proinflammatory cytokine production keratinocytes. However, downstream protein target(s) that link radiation to activation of signaling pathways responsible for expression have not been fully elucidated. In this study, we report a novel role transglutaminase 2 (TG2), member TG enzyme family whose activities are critical cornified envelope formation, mediating UV-induced inflammation. Our results showed TG2-deficient...
Radical cystectomy with preoperative cisplatin-based neoadjuvant chemotherapy (NAC) is the standard care for muscle-invasive bladder cancers (MIBCs). However, complete response rate to this modality remains relatively low, and current clinicopathologic molecular classifications are inadequate predict NAC in patients MIBC. Here, we demonstrate that dysregulation of glutathione (GSH) pathway fundamental MIBC resistance. Comprehensive analysis multicohort transcriptomes reveals GSH metabolism...
Aberrant activation of transglutaminase 2 (TGase2) contributes to a variety protein conformational disorders such as neurodegenerative diseases and age-related cataracts. The accumulation improperly folded proteins in the endoplasmic reticulum (ER) triggers unfolded response (UPR), which promotes either repair or degradation damaged proteins. Inadequate UPR results aggregation that may contribute development degenerative diseases. TGase2 is calcium-dependent enzyme irreversibly modifies by...
Glucocorticoids play a major role in the development of muscle atrophy various medical conditions, such as cancer, burn injury, and sepsis, by inhibiting insulin signaling. In this study, we report new pathway which glucocorticoids reduce levels upstream signaling components downregulating transcription gene encoding caveolin-1 (CAV1), scaffolding protein present caveolar membrane. Treatment with glucocorticoid dexamethasone (DEX) decreased CAV1 Cav1 mRNA expression, concomitant reduction...
IL-22 is a pro- and anti-inflammatory cytokine that mainly produced by T cells NK cells. Recent studies have reported the increased number of producing in patients with autoimmune noninfectious uveitis; however, correlation between uveitis remains unclear. In this study, we aimed to determine specific role its receptor pathogenesis uveitis. Serum concentration was significantly patients. IL-22Rα expressed retinal pigment epithelial cell line, ARPE-19. To examine effect IL-22, ARPE-19 treated...
Transglutaminase 4 is a member of enzyme family that catalyzes calcium-dependent posttranslational modification proteins. Although transglutaminase has been shown to have prostate-restricted expression pattern, little known about the biological function in human. To gain insight into its role prostate, we analyzed status human benign prostate hyperplasia (BPH) and cancer (PCa). Unexpectedly, RT-PCR nucleotide sequence analysis showed four alternative splicing variants 4: 4-L, -M (-M1 -M2)...
Abstract Objective 4‐n‐butylresorcinol is a competitive inhibitor of tyrosinase and has been used as an antimelanogenic agent. However, its inhibition mechanism in intact cells not fully understood. To elucidate the cellular mechanism, we compared vitro vivo inhibitory effects on activity. Methods B16F10 melanoma were cultured media containing α ‐ MSH presence or absence 4‐n‐butylresorcinol. Tyrosinase mRNA levels, protein levels activity by real‐time PCR , immunostaining combined with...
Abstract Keratinocyte-derived cytokines and chemokines amplify psoriatic inflammation by recruiting IL-17-producing CCR6 + γδT-cells neutrophils. The expression of these mainly depends on NF-κB activity; however, the pathway that activates in response to triggering factors is poorly defined. Here, we show transglutaminase 2 (TG2), previously reported elicit a T H 17 increasing IL-6 mouse model lung fibrosis, mediates upregulation activating imiquimod (IMQ)-treated keratinocytes....
Transglutaminase 2 (TG2) is a calcium-dependent enzyme that catalyzes the transamidation reaction. There conflicting evidence on role of TG2 in apoptosis. In this report, we show increases response to low level oxidative stress, whereas diminishes under high stress conditions. Monitoring expression, activity and calcium concentration cells treated with A23187 revealed initial rise activates but subsequent calcium-overload induces degradation via calcium-mediated polyubiquitination. These...
The activation of transglutaminase 2 (TG2), an enzyme that catalyzes post-translational modifications proteins, has been implicated in apoptosis, cell adhesion and inflammatory responses. We previously reported intracellular TG2 is activated under oxidative stress conditions, such as ultraviolet irradiation, ischemia-reperfusion, hypoxia. In this study, we examined the effect genotoxic on activity using doxorubicin which generates reactive oxygen species lead to double-strand breakage DNA....
The activation of transglutaminase 2 (TG2) by oxidative stress through TGFβ has been reported to play a crucial role in cataract formation. authors investigated whether TG2 is involved selenite-induced formation rats and cysteamine, chemical inhibitor TG2, can prevent this model.Intracellular activity was monitored human lens epithelial cell (HLE-B3) line cultured rat lenses after treatment with selenite. Rat pups (13 days old) were injected subcutaneously sodium selenite (Na(2)SeO(3); 20...
Abstract Transglutaminase 2 (TG2) performs multiple reactions, including transamidation, and also plays a role in signal transduction as GTP-binding protein. In this study, we reveal that TG2 controls osteoclast differentiation bone homeostasis mice. Osteoclasts specifically expressed the isoform among eight TG family members. Suppression expression with siRNA led to increased formation from primary mouse precursor cells response receptor activator of nuclear factor kappaB ligand (RANKL)....
Transglutaminase 2 (TG2) is a ubiquitously expressed enzyme that catalyzes crosslinking, polyamination or deamidation of glutamine residues in proteins. It has been reported TG2 involved the pathogenesis various inflammatory diseases including celiac disease, pulmonary fibrosis, cystic multiple sclerosis and sepsis. Recently, using mouse model bleomycin-induced lung we showed required to trigger inflammation via induction T helper type 17 (Th17) cell differentiation response tissue damage....