A5041866274- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Advanced Radiotherapy Techniques
- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- CRISPR and Genetic Engineering
- Radiation Dose and Imaging
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Osteoarthritis Treatment and Mechanisms
- Sirtuins and Resveratrol in Medicine
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Radiation Effects and Dosimetry
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Reconstructive Surgery and Microvascular Techniques
- Angiogenesis and VEGF in Cancer
- Head and Neck Cancer Studies
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Surgical site infection prevention
Greater Poland Cancer Center
2013-2024
Poznan University of Medical Sciences
2012-2021
Klinikum Klagenfurt
2016
The tumor microenvironment (TME) is a complex ecosystem of cells, signaling molecules, and extracellular matrix components that profoundly influence cancer progression. Among the key players in TME, cancer-associated fibroblasts (CAFs) have gained increasing attention for their diverse influential roles. CAFs are activated found abundantly within TME various types. contribute significantly to progression by promoting angiogenesis, remodeling matrix, modulating immune cell infiltration. In...
Reprogramming to induced pluripotent stem cells (iPSCs) and differentiation of (PSCs) are regulated by epigenetic machinery. Tripartite motif protein 28 (TRIM28), a universal mediator Krüppel-associated box domain zinc fingers (KRAB-ZNFs), is known regulate both processes; however, the exact mechanism identity participating KRAB-ZNF genes remain unknown. Here, using reporter system, we show that TRIM28/KRAB-ZNFs alter DNA methylation patterns in addition H3K9me3 cause stable gene repression...
Lentiviral vectors are efficient vehicles for stable gene transfer in both dividing and non-dividing cells. This feature among others makes lentiviral a powerful tool molecular research. However, the use of lentiviruses research studies clinical trials requires precise validated titration method. In this study, we describe qPCR-based approach estimation vector titer (pLV-THM-GFP). The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) albumin genes as templates an SYBR...
Although the effects of high dose radiation on human cells and tissues are relatively well defined, there is no consensus regarding low very doses organism. Ionizing has been shown to induce gene mutations chromosome aberrations which known be involved in process carcinogenesis. The induction secondary cancers a challenging long-term side effect oncologic patients treated with radiation. Medical sources like intensity modulated radiotherapy used cancer treatment computed tomography...
Invasive oncological procedures affect the remaining tumor cells by increasing their survival, proliferation, and migration through induction of wound healing response. The phenomena local relapse after breast-conserving surgery (BCS) has resulted in a series research clinical trials with aim assessing whether localized intraoperative radiotherapy (IORT), may be beneficial inhibiting recurrences. Therefore, it is essential to assess impact modulating immunological response process. Thus, we...
The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in treatment joint diseases. A number protocols vitro differentiation iPSC chondrocytes regenerative purposes have been recently described. However, order to use these clinics, elimination animal serum and feeder essential. In our study, a strictly defined controllable protocol...
Uveal melanoma (UM) is the most common primary tumor of eye diagnosed in adults, associated with a high risk metastasis and thereby, poor prognosis. Among known factors for development metastatic disease loss BAP1 expression chromosome 3 monosomy tumor. However, levels specific micro RNAs (miRNA) tissue may also serve as valuable marker determining patients uveal melanoma. In our study, we analyzed miRNA data cases selected from The Cancer Genome Atlas study on melanoma, determined panel 15...
MicroRNAs (miRNA) play an essential role in the regulation of gene expression and influence signaling networks responsible for several cellular processes like differentiation pluripotent stem cells. Despite studies on neurogenesis process, no global analysis microRNA during induced cells (iPSC) to neuronal (NSC) has been done. Therefore, we compared profile iPSC lines NSC derived from them, using microarray-based analysis. Two different protocols formation were used: Direct two-step via...
Wound fluids (WF) are believed to play a role in the local recurrences by inducing an inflammatory process scar tissue area. Given that most relapse primary breast cancer patients occur within area, researchers have investigated whether localized radiotherapy, such as intraoperative radiotherapy (IORT), could be more effective than postoperative RT inhibiting tumor recurrence. The epithelial-mesenchymal transition (EMT) program plays critical promoting metastasis epithelium-derived...
Background: Uveal melanoma (UM) is the most common intraocular tumour in adults with a poor prognosis and extremely high mortality rate due to development of metastatic disease. However, despite relatively good knowledge about histological genetic risk factors for metastasis development, there no specific biomarker that would allow early detection UM progression. Recently, exosomes their molecular cargo have been widely studied search potential biomarkers several cancers. The purpose this...
Human induced pluripotent stem cells (hiPSCs) play an important role in research regarding regenerative medicine. Particularly, chondrocytes differentiated from hiPSCs seems to be a promising solution for patients suffering osteoarthritis. We decided perform chondrogenesis three-week monolayer culture. Based on transcriptome analysis, hiPSC-derived (ChiPS) demonstrate the gene expression profile of early chondrogenesis. Chondrogenic progenitors obtained by our group are characterized...
Breast cancer is the most common occurring in women. The standard of breast treatment based on breast-conserving surgery with administration adjuvant whole radiotherapy. Research shows that in-breast relapse likely to occur tumour bed, i.e. around scar. Intraoperative radiotherapy (IORT), which radiation delivered reduces risk local recurrence not only through direct cell killing, but also modification microenvironment. Additionally IORT modifies composition and biological activity surgical...
In the adrenal cortex, corticotropin induces expression of several genes encoding proteins involved in synthesis and intracellular transport steroid hormones via protein kinase A (PKA) signalling pathway, this process is mediated by steroidogenic factor-1 (SF-1). This study was designed to elucidate influence PKA PKC pathways on SF-1 gene mouse adrenocortical cells, line Y-1. It has also been attempted answer question whether or not plays a role PKA-induced LIPE hormone-sensitive...
Breast cancer is the most common malignant disease occurring in women. Conservative breast surgery followed by radiation therapy currently standard treatment for this type of cancer. The majority metastases occur within scar, which initiated a series studies. As result, clinical trials aimed to assess whether localized radiotherapy, as intraoperative radiotherapy (IORT), may more effective inhibiting formation local recurrence compared with postoperative whole radiotherapy. present study...
In patients with breast cancer who undergo breast-conserving surgery (BCS), more than 90% of local recurrences occur in the same quadrant as primary cancer. Surgical wound fluids (SWF) are believed to play a role this process by inducing an inflammatory scar tissue area. Despite strong clinical data demonstrating benefits intraoperative radiotherapy (IORT), biological basis underlying remains poorly understood. Ionizing radiation (IR) directly affects cells damaging DNA, thereby altering...