A5053453662- Malaria Research and Control
- Computational Drug Discovery Methods
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Parasite Biology and Host Interactions
- Aquatic Invertebrate Ecology and Behavior
- Moringa oleifera research and applications
- Phytochemical compounds biological activities
- HIV/AIDS drug development and treatment
- Insect Pest Control Strategies
- Nigella sativa pharmacological applications
- Bioactive Compounds and Antitumor Agents
- Pharmacological Effects of Natural Compounds
- HIV Research and Treatment
- Mollusks and Parasites Studies
- Diverse Scientific Research Studies
University of Ghana
2020-2023
MRC Unit the Gambia
2017-2023
Wellcome Sanger Institute
2023
Keele University
2017-2021
Medical Research Council
2019
Antimalarial interventions have yielded a significant decline in malaria prevalence The Gambia, where artemether-lumefantrine (AL) has been used as first-line antimalarial for decade. Clinical Plasmodium falciparum isolates collected from 2012 to 2015 were analyzed ex vivo susceptibility and genotyped drug resistance markers (pfcrt K76T, pfmdr1 codons 86, 184, 1246, pfk13) microsatellite variation. Additionally, allele frequencies of single nucleotide polymorphisms (SNPs) other...
Abstract Background Artemether/lumefantrine is the most commonly used artemisinin-based combination treatment (ACT) for malaria in sub-Saharan Africa. Drug resistance to ACT components a major threat elimination efforts. Therefore, rigorous monitoring of drug efficacy required adequate management and sustain effectiveness ACTs. Objectives This study identified described genomic loci that correlate with differences ex vivo responses natural Plasmodium falciparum isolates from The Gambia...
Development of resistance to deployed antimalarial drugs is inevitable and needs prompt continuous discovery novel candidate drugs. Therefore, the activity 125 compounds from Medicine for Malaria Ventures (MMV) pathogen box was determined. Combining standard IC50 normalised growth rate inhibition (GR50) analyses, we found 16 22 had higher potencies than CQ respectively. Seven with relatively high (low GR50 IC50) against P. falciparum 3D7 were further analysed. Three these tested on 10...
Molluscs are a highly speciose phylum that exhibits an astonishing array of colours and patterns, yet relatively little progress has been made in identifying the underlying genes determine phenotypic variation. One prominent example is land snail Cepaea nemoralis for which classical genetic studies have shown around nine loci, several physically linked inherited together as 'supergene', control shell colour banding polymorphism. As first step towards involved, we used whole-genome...
Monitoring of Plasmodium falciparum sensitivity to antimalarial drugs in Africa is vital for malaria elimination. However, the commonly used ex vivo/in vitro 50% inhibitory concentration (IC50) test gives inconsistent results several antimalarials, while alternative ring-stage survival assay (RSA) artemisinin derivatives has not been widely adopted. Here, we applied an two-color flow cytometry-based parasite rate (PSRA) detect vivo tolerance P. isolates from The Gambia. PSRA infers viability...
The use of plant-derived natural products for the treatment tropical parasitic diseases often has ethnopharmacological origins. As such, plants grown in temperate regions remain largely untested novel anti-parasitic activities. We describe here a screen PhytoQuest Phytopure library, source comprising over 600 purified compounds from zone plants, against vitro culture systems Plasmodium falciparum, Leishmania mexicana, Trypanosoma evansi and T. brucei. Initial revealed 6, 65, 15 18 compounds,...
The malaria parasite Plasmodium falciparum utilizes multiple alternative receptor-ligand interactions for the invasion of human erythrocytes. While some P. clones make use sialic acid (SA) residues on surface glycophorin receptors to invade erythrocyte, others independent residues. We hypothesized that over years, intensified control interventions and declining prevalence in Gambia have resulted a selection parasites with dominant pathways ligand expression profiles.Blood samples were...
ABSTRACT Two-color flow cytometry(2cFCM) is the most accessible method for phenotyping parasite invasion. However, current protocols require samples of field isolates at ∼1% parasitemia assay set-up, which are becoming more uncommon in low transmission settings. Current protocols, therefore, have to be adapted if must continued applicability this era elimination. Optimizing protocol requires addressing; interference from young uninfected RBCs background fluorescence and biased phenotypes due...