A5037292353- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Mechanisms of cancer metastasis
- RNA modifications and cancer
- Cancer Research and Treatments
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Reproductive Biology and Fertility
- RNA and protein synthesis mechanisms
- Tissue Engineering and Regenerative Medicine
- Protein Degradation and Inhibitors
- Cancer Mechanisms and Therapy
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Prostate Cancer Diagnosis and Treatment
- Microtubule and mitosis dynamics
Hunan University
2020-2024
Shenzhen University
2023-2024
Roswell Park Comprehensive Cancer Center
2016-2023
State Key Laboratory of Chemobiosensing and Chemometrics
2023
Huazhong Agricultural University
2005-2019
Ministry of Agriculture and Rural Affairs
2019
The University of Texas MD Anderson Cancer Center
2014-2018
Center of Hubei Cooperative Innovation for Emissions Trading System
2018
Forest Institute
2017
Wake Forest University
2017
Abstract MicroRNAs play important roles in regulating tumour development, progression and metastasis. Here we show that one of the miR-200 family members, miR-141, is under-expressed several prostate cancer (PCa) stem/progenitor cell populations both xenograft primary patient tumours. Enforced expression miR-141 CD44 + bulk PCa cells inhibits stem properties including holoclone sphere formation, as well invasion, suppresses regeneration Moreover, enforces a strong epithelial phenotype with...
Abstract The prostate gland mainly contains basal and luminal cells constructed as a pseudostratified epithelium. Annotation of epithelial transcriptomes provides foundation for discoveries that can impact disease understanding treatment. Here we describe genome-wide transcriptome analysis human benign prostatic populations using deep RNA sequencing. Through molecular biological characterizations, show the differential gene-expression profiles account their distinct functional properties....
Background Tumor-associated macrophages are mainly polarized into the M2 phenotype, remodeling tumor microenvironment and promoting progression by secreting various cytokines. Methods Tissue microarray consisting of prostate cancer (PCa), normal prostate, lymph node metastatic samples from patients with PCa were stained Yin Yang 1 (YY1) CD163. Transgenic mice overexpressing YY1 constructed to observe tumorigenesis. Furthermore, in vivo vitro experiments, including CRISPR-Cas9 knock-out, RNA...
METTL3, a primary methyltransferase catalyzing the RNA N6-methyladenosine (m6A) modification, has been identified as an oncogene in several cancer types and thus nominated potentially effective target for therapeutic inhibition. However, current options using this strategy are limited. In study, we targeted protein-protein interactions at METTL3-METTL14 binding interface to inhibit complex formation subsequent catalysis of m6A modification. Among candidate peptides, RM3 exhibited highest...
Abstract Expression of androgen receptor (AR) in prostate cancer (PCa) is heterogeneous but the functional significance AR heterogeneity remains unclear. Screening ~200 castration-resistant PCa (CRPC) cores and whole-mount sections (from 89 patients) reveals 3 expression patterns: nuclear (nuc-AR), mixed nuclear/cytoplasmic (nuc/cyto-AR), low/no (AR −/lo ). Xenograft modeling demonstrates that + CRPC enzalutamide-sensitive resistant. Genome editing-derived AR-knockout LNCaP cell clones...
The role of dysregulation mRNA alternative splicing (AS) in the development and progression solid tumors remains to be defined. Here we describe first comprehensive AS landscape spectrum human prostate cancer (PCa) evolution. We find that severity correlates with disease establish intron retention as a hallmark PCa stemness aggressiveness. Systematic interrogation 274 splicing-regulatory genes (SRGs) uncovers prevalent genomic copy number variations (CNVs), leading mis-expression ~68% SRGs...
Abstract Purpose: We have shown that the phenotypically undifferentiated (PSA−/lo) prostate cancer cell population harbors long-term self-renewing stem cells (CSC) resist castration, and a subset of within PSA−/lo bearing ALDHhiCD44+α2β1+ phenotype (Triple Marker+/TM+) is capable robustly initiating xenograft tumors in castrated mice. The goal current project to further characterize biologic properties TM+ population, particularly context propagating castration-resistant (CRPC). Experimental...
Myelodysplastic syndromes (MDS) are age-related myeloid neoplasms with increased risk of progression to acute leukemia (AML). The mechanisms transformation MDS AML poorly understood, especially in relation the aging microenvironment. We previously established an mDia1/miR-146a double knockout (DKO) mouse model phenocopying MDS. These mice develop pancytopenia oversecretion proinflammatory cytokines. Here, we found that most DKO underwent leukemic at 12-14 months age. showed myeloblast...
Estrogen receptor is a nuclear superfamily member of transcriptional activators that regulate gene expression by recruiting diverese coregulators. The Mediator complex central coactivator acts as bridge between and RNA polymerase II. MED1 (Mediator subunit 1) the key directly interacts with estrogen to mediate its functions both in vitro vivo. Interestingly, our previous biochemical analyses indicated exists only subpopulation enriched number distinct subunits Here, we report ARGLU1...
The pluripotency transcription factor NANOG has been implicated in tumor development, and NANOG-expressing cancer cells manifest stem cell properties that sustain homeostasis, mediate therapy resistance fuel progression. However, how converges on somatic circuitry to trigger oncogenic reprogramming remains obscure. We previously reported inducible expression propels the emergence of aggressive castration-resistant prostate phenotypes. Here we first show endogenous is required for growth...
Human cancers exhibit significant cellular heterogeneity featuring tumorigenic cancer stem cells (CSCs) in addition to more differentiated progeny with limited tumor-initiating capabilities. Recent studies suggest that microRNAs (miRNAs) regulate CSCs and tumor development. A previous library screening for differential miRNA expression CD44+ (and other) prostate CSC vs. non-CSC populations identified miR-199a-3p be among the most highly under-expressed miRNAs CSCs. In this study, we...
Histopathological heterogeneity is a hallmark of prostate cancer (PCa). Using spatial and parallel single-nucleus transcriptomics, we report an androgen receptor (AR)-positive but neuroendocrine-null primary PCa subtype with morphologic molecular characteristics small cell carcinoma. Such cell-like (SCLPC) clinically aggressive low AR, high stemness proliferation, activity. Molecular characterization prioritizes protein translation, represented by up-regulation many ribosomal genes, SP1,...
Abstract METTL3, a primary methyltransferase catalyzing the RNA N6‐methyladenosine (m6A) modification, has been identified as an oncogene in several cancer types and thus nominated potentially effective target for therapeutic inhibition. However, current options using this strategy are limited. In study, we targeted protein–protein interactions at METTL3–METTL14 binding interface to inhibit complex formation subsequent catalysis of m6A modification. Among candidate peptides, RM3 exhibited...
Diaphanous-related formin 3 (DIAPH3) is a pivotal member of the family and serves as crucial regulator actin filament assembly. As such, DIAPH3 plays an integral role in variety cellular processes including cytokinesis, cell migration, intracellular transport. Given its fundamental importance maintaining cytoskeletal dynamics, functionally associated with numerous physiological pathological conditions, particularly cancer. In this review, we explore structural functional characteristics...
Mitogen-activated protein kinase (MAPK) and maturation/M phase promoting factor (MPF) play crucial roles in oocyte meiotic maturation mammals. However, the underlying molecular mechanisms have not been addressed. In this study, effects of MEK/MAPK pathway inhibitor U0126 MPF roscovitine on maternal gene expression pig cumulus-oocyte complexes (COCs) denuded oocytes (DOs) were investigated. Both inhibitors can reversibly block resumption meiosis oocytes. COCs or DOs initially cultured...
The existence of slow-cycling luminal cells in the prostate has been suggested, but their identity and functional properties remain unknown. Using a bigenic mouse model to earmark, isolate, characterize quiescent stem-like cells, we identify label-retaining cell (LRC) population layer as progenitors. Molecular biological characterizations show that these LRCs are significantly enriched proximal prostate, exhibit relative dormancy, display bipotency both vitro vivo assays, express...
Abstract Signal transducers and activators of transcription‐3 (Stat3) plays a central role in interleukin‐6 (IL‐6)‐mediated cell proliferation by inhibiting apoptosis variety types. MicroRNA‐21 (miRNA‐21), ubiquitous miRNA, acts as an anti‐apoptotic factor that seems to be indirectly but strictly linked Stat3. In order determine whether the IL‐6 induced Stat3 anti‐apoptosis pathway is with miRNA‐21, we first determined effects recombinant mouse on expression, embryo viability, mRNA levels...
Abstract Ndc80 (called Hec1 in human), the core component of complex, is involved regulation both kinetochore-microtubule interactions and spindle assembly checkpoint mitosis; however, its role meiosis remains unclear. Here, we report expression, localization, possible functions mouse oocyte meiosis. mRNA levels gradually increased during Immunofluorescent staining showed that was restricted to germinal vesicle associated with microtubules from Pro-MI MII stages. localized on asters...