A5090832266- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Per- and polyfluoroalkyl substances research
- Influenza Virus Research Studies
- Air Quality and Health Impacts
- interferon and immune responses
- Respiratory viral infections research
- Toxic Organic Pollutants Impact
- Vector-Borne Animal Diseases
- Retinal and Optic Conditions
- Virology and Viral Diseases
- Animal Disease Management and Epidemiology
- Effects and risks of endocrine disrupting chemicals
- vaccines and immunoinformatics approaches
- SARS-CoV-2 detection and testing
- RNA Research and Splicing
- RNA modifications and cancer
- T-cell and Retrovirus Studies
- T-cell and B-cell Immunology
- Thermal Regulation in Medicine
- Vagus Nerve Stimulation Research
- COVID-19 Impact on Reproduction
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
Cornell University
2020-2025
New York State College of Veterinary Medicine
2021-2025
University of California, Davis
2018
The COVID-19 pandemic caused by the SARS-CoV-2 virus remains a global public health crisis. Although widespread vaccination campaigns are underway, their efficacy is reduced owing to emerging variants of concern
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing the ongoing global pandemic associated with morbidity and mortality in humans. Although disease severity correlates immune dysregulation, cellular mechanisms of inflammation pathogenesis COVID-19 remain relatively poorly understood. Here, we used mouse-adapted SARS-CoV-2 strain MA10 to investigate role adaptive cells disease. We found that while infected wild-type mice lost ~10% weight by 3 4 days postinfection,
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses lead to severe illnesses death in humans, exacerbated individuals with underlying health conditions, remaining substantial global public concerns. Here, we developed a bivalent replication-incompetent single-cycle pseudotyped vesicular stomatitis virus vaccine that incorporates both prefusion-stabilized SARS-CoV-2 spike protein lacking furin cleavage site full-length A neuraminidase protein. Vaccination of...
The COVID-19 pandemic has been driven by SARS-CoV-2 variants with enhanced transmission and immune escape. Apart from extensive evolution in the Spike protein, non-Spike mutations are accumulating across entire viral genome their functional impact is not well understood. To address contribution of these mutations, we reconstructed genomes recent Omicron disabled expression (replicons) to systematically compare RNA replication capabilities independently Spike. We also used a single reference...
Pathology studies of SARS-CoV-2 Omicron variants concern (VOC) are challenged by the lack pathogenic animal models. While BA.1 and BA.2 replicate in K18-hACE2 transgenic mice, they cause minimal to negligible morbidity mortality, less is known about more recent VOC. Here, we show that contrast BA.1, BA.5-infected mice exhibited high levels correlating with higher early viral loads. Neither nor BA.5 replicated brains, unlike most prior Only BA.5–infected substantial weight loss, pathology...
Experimental vaccines for the deadly zoonotic Nipah (NiV), Hendra (HeV), and Ebola (EBOV) viruses have focused on targeting individual viruses, although their geographical bat reservoir host overlaps warrant creation of multivalent vaccines. Here we explored whether replication-incompetent pseudotyped vesicular stomatitis virus (VSV) virions or NiV-based virus-like particles (VLPs) were suitable vaccine platforms by co-incorporating multiple surface glycoproteins from NiV, HeV, EBOV onto...
Angiotensin-converting enzyme 2 (ACE2) is the cell-surface receptor for Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2). While its central role in Disease 2019 (COVID-19) pathogenesis indisputable, there remains significant debate regarding of this transmembrane carboxypeptidase disease course. These include soluble versus membrane-bound ACE2, as well ACE2-independent mechanisms that may contribute to viral spread. Testing these roles requires vivo models. Here, we report...
ABSTRACT COVID-19 can cause neurological symptoms such as fever, dizziness, and nausea. However, of SARS-CoV-2 infection have been hardly assessed in mouse models. In this study, we infected two commonly used wild-type lines (C57BL/6J 129/SvEv) a 129S calcitonin gene-related peptide (αCGRP) null-line with mouse-adapted demonstrated signs including We then evaluated whether CGRP receptor antagonist, olcegepant, “gepant” antagonist migraine treatment, could mitigate acute neuroinflammatory...
Summary The COVID-19 pandemic caused by the SARS-CoV-2 virus remains a global public health crisis. Although widespread vaccination campaigns are underway, their efficacy is reduced against emerging variants of concern (VOCs) 1,2 . Development host-directed therapeutics and prophylactics could limit such resistance offer urgently needed protection VOCs 3,4 Attractive pharmacological targets to impede viral entry include type-II transmembrane serine proteases (TTSPs), as TMPRSS2, whose...
The COVID-19 pandemic has been driven by SARS-CoV-2 variants with enhanced transmission and immune escape. Apart from extensive evolution in the Spike protein, non-Spike mutations are accumulating across entire viral genome their functional impact is not well understood. To address contribution of these mutations, we reconstructed genomes recent Omicron disabled expression (replicons) to systematically compare RNA replication capabilities independently Spike. We also used a single reference...
Perfluorooctane sulfonic acid (PFOS) is member of a class molecules with fluorinated carbon chains known as polyfluoroalkyls. PFOS have been used to produce variety industry and comsumer uses. However, significant concern that it accumulates in the environment, including animals humans, potential immunosuppressant. Here we analyze immune homeostasis mice following chronic exposure at levels up those historically found manufacturing workers. Mice were exposed 0.15, 1.5, 15, or 50 µg /kg for...
Endothelial damage and vascular pathology have been recognized as major features of COVID-19 since the beginning pandemic. Two main theories regarding how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) damages endothelial cells causes proposed: direct viral infection or indirect mediated by circulating inflammatory molecules immune mechanisms. However, these proposed mechanisms remain largely untested in vivo . In present study, we utilized a set new mouse genetic tools...
Female genital epithelial cells cover the tract and provide first line of protection against infection with sexually transmitted pathogenic viruses. These normally are impervious to HIV-1. We report that coinfection by HIV-1 another virus, human T-lymphotropic virus 1 (HTLV-1), led production had expanded cell tropism was able directly infect primary vaginal cervical cells. blocked neutralizing antibodies HTLV-1 envelope (Env) protein, indicating mediated through Env pseudotyping Active...
Abstract Enveloped viruses can cause devastating zoonotic diseases and are the most likely to global pandemics. We identified a new class of small-molecule sulfur-containing antiviral compounds (XM series) that broadly inhibit enveloped viruses. The antivirals’ mechanism action was explored via various multidisciplinary approaches, concluding XM antivirals alter membrane lipid chemical compositions, increase order deep within hydrophobic region bilayer, phase transition temperatures. Such...
Abstract Perfluorohexane sulfonate (PFHxS) is a member of the per- and polyfluoroalkyls (PFAS) superfamily molecules, characterized by their fluorinated carbon chains use in wide range industrial applications. PFHxS perfluorooctane are able to accumulate environment humans with approximated serum elimination half-life several years. More recently, some PFAS compounds have also been suggested as potential immunosuppressants. In this study, we analyze immune cell numbers mice following 28-d...
Endothelial damage and vascular pathology have been recognized as major features of COVID-19 since the beginning pandemic. Two main theories regarding how Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) damages endothelial cells causes proposed: direct viral infection or indirect mediated by circulating inflammatory molecules immune mechanisms. However, these proposed mechanisms remain largely untested in vivo. Here, we utilized a set new mouse genetic tools 1 developed our lab...
Abstract Perfluorinated compounds, such as Perfluoroctane sulfonic acid (PFOS) are compounds containing carbon chains where hydrogens have been replaced with fluorines, and belong to a larger family known Per- polyfluoroalkyl substances (PFAS). The strength of the carbon–fluorine bond makes perfluorinated extremely resistant environmental degradation. Due persistent nature PFOS, research has directed elucidating possible health effects PFOS on humans laboratory animals. Here we explored...
Abstract COVID-19 can result in neurological symptoms such as fever, headache, dizziness, and nausea. However, signs of SARS-CoV-2 infection have been hardly assessed mouse models. Here, we infected two commonly used wildtype mice lines (C57BL/6 129S) with mouse-adapted demonstrated including motion- related dizziness. We then evaluated whether the Calcitonin Gene-Related Peptide (CGRP) receptor antagonist, olcegepant, migraine treatment could mitigate acute neuroinflammatory responses to...