The aim of this study was to compare the effect increasing concentrations (0, 1, 2, 4%) sea buckthorn oil (SBO) on structural, physicochemical, release, and antioxidant properties glycerol-plasticized sodium casein (NaCAS) gelatin (GEL) films. Ultrasonic treatment ensured effective homogenization SBO in both types emulsions, resulting yellow-tinted semi-opaque films with relatively low micro-roughness. Generally, GEL demonstrated lower UV barrier solubility but exhibited higher compactness,...

Abstract Entomopathogenic bacteria from the genera Photorhabdus and Xenorhabdus are closely related Gram-negative bacilli family Enterobacteriaceae (γ- Proteobacteria ). They establish obligate mutualistic associations with soil nematodes Steinernema Heterorhabditis to facilitate insect pathogenesis. The research of these two bacterial is focused mainly on their unique interactions different animal hosts, i.e. insects. So far, studies collected around world have contributed an increase in...

Unlike many biopolymers, α-1,3-glucan (α-1,3-GLU) is water-insoluble, making it a promising candidate for the production of moisture-resistant films with applications in biodegradable packaging, biomedicine, and cosmetics. This study aimed to characterize structural, physicochemical (water affinity, optical, mechanical), biodegradation properties film made from α-1,3-GLU extracted Laetiporus sulphureus. The was fabricated through alkaline dissolution, casting, drying, washing remove residual...

Microsporidia Nosema are transferred among bees via the faecal-oral route. spp. spores have been detected on flowers and to hives along with bee pollen. The aim of present study was determine whether microsporidia by air in an apiary, a control area (without presence colonies), and/or laboratory during cage experiments artificially infected bees. novel way transmission investigated volumetric method using Hirst-type aerobiological sampler located ground Botanical Garden floor. Concurrently,...

The mechanisms of action the complex including entomopathogenic nematodes genera Steinernema and Heterorhabditis their mutualistic partners, i.e., bacteria Xenorhabdus Photorhabdus, have been well explained, commercialized as biological control agents against many soil insect pests. However, little is known regarding nature relationships between these gut microbiota infected insects. In present study, 900 bacterial isolates that were obtained from midgut samples Melolontha melolontha larvae...

Polymer blending and incorporation of active substances offer a possibility generation novel packaging materials with interesting features. Astaxanthin is one the most powerful antioxidants. Hence, in this study, water-soluble AstaSana astaxanthin (AST) was incorporated into 75/25 gum arabic/gelatin (GAR75/GEL25) soy polysaccharides/gelatin (WSSP75/GEL25) blend films different concentrations (0, 0.25%, 0.5%, 1%). Microscope images showed good compatibility between polysaccharides GEL. Basing...

Ca2+ can boost protein-protein interactions and, if present at an appropriate level, potentially improve some physicochemical properties of protein-based gels and films. This study aimed to determine the effects CaCl2 (0%–0.05% w/w) on microstructural, optical, water affinity, mechanical characteristics glycerol (Gly)- sorbitol (Sor)-plasticized pea protein isolate (PPI)-based caused darkening a color shift films from yellow yellow-green. Additionally, decreased light transmission,...

The fertility and productive value of soil are closely related to the physical chemical properties as well its biological activity. This activity is intensity microbially catalysed processes transformation organic mineral substances contained in soil. These correlated with abundance biodiversity microorganisms, especially bacteria, enzymes produced by them. In this article we have compared some physicochemical derived from conventional farms microbial communities inhabiting these ecosystems....

Nisin (NIS) Z was incorporated (0.05 %, 0.1 0.2 %) into edible films based on chitosan lactate (CHL) and 75/25 blends of polysaccharides (corn starch (CS), wheat (WS), oxidized potato (OPS), pullulan (PUL)) with CHL. The increase in the NIS/polymer ratio promoted diffusion-driven release. Compared fully dissolvable CHL PUL/CHL carriers, starch/CHL had limited solubility (≈27-37 and, consequently, ensured slower/incomplete release NIS. assayable NIS half-release times, determined water,...

Osteoarthritis (OA) is one of the most prevalent diseases osteoarticular system. Progressive destruction joints accompanied by development pathological changes in muscle tissue, i.e., weakening, atrophy, and remodelling (sarcopenia). The aim present study to assess impact physical activity on musculoskeletal system an animal model early degenerative lesions knee joint. involved 30 male Wistar rats. animals were allocated three subgroups 10 each. Each from received sodium iodoacetate...

Three strains of symbiotic bacteria were isolated from an entomopathogenic nematode Steinernema poinari retrieved soil in eastern Poland. Using 16S rDNA, recA, gltX, gyrB, and dnaN gene sequences for phylogenetic analysis, these shown to belong the species Xenorhabdus bovienii. The nucleotide identity between studied S. microsymbionts other X. bovienii calculated rDNA concatenated four protein-coding genes was 98.7-100% 97.9-99.5%, respectively. phenotypic properties isolates also supported...

The use of complementary visualization and measurement techniques allowed accurate description quantification changes in the intestinal mucosal architecture provided a comprehensive outlook on dynamics remodelling maturation processes layer taking place small intestine piglets from birth to weaning. aim study was examine early postnatal development pigs. Three were used: scanning electron microscopy (measurements villus density shape, height enterocytes microvilli, cell exfoliation, location...

Root-feeding Amphimallon solstitiale larvae and certain other scarab beetles are the main soil-dwelling pests found in Europe, while entomopathogenic nematodes (EPN) have been used as a biocontrol agent against these species. Our study provides first detailed characterization of bacterial community midgut wild A. larvae, based on nanopore sequencing 16S rRNA gene. In whole dataset, we detected 2586 different genera 11,641 species, with only 83 diverse shared by all studied individuals, which...

Abstract A new species of entomopathogenic nematodes, Steinernema sandneri n. sp., was recovered by baiting from Poland. Its morphological traits indicate that the is a member feltiae-kraussei group. body length 843 (708–965) μm, more anterior position excretory pore (56 μm), and lower D% value (40 vs > 46) discriminate this most other group members. The first-generation males S. sp. can be distinguished clade members 60 μm long spicule, relatively gubernaculum (GS% = 79), (80 μm)....

Hydrophobization could improve the moisture resistance of biopolymer materials, depending on methods and materials used, providing benefits for packaging applications. The aim this study was to compare effect increasing concentrations (0-2%) candelilla wax (CW) oleic acid (OA) microstructural physicochemical properties, including water affinity, glycerol-plasticized pea protein isolate (PPI) films. OA acidified film-forming solution increased its viscosity more effectively than CW. At...

: Thoracolumbar and lumbar spine injuries account for 30-60% of spinal fractures, especially at the thoracolumbar junction. Conservative treatment is recommended stable fractures without neurological symptoms, but studies suggest surgical intervention may offer better outcomes. However, there no consensus on best stabilization method.

Hydrophobization could improve the moisture resistance of biopolymer-based materials, depending on methods and materials used, providing benefits for packaging applications. The aim this study was to compare effect increasing concentrations (0–2.0%) candelilla wax (CW) oleic acid (OA) structural physicochemical properties, including water affinity, glycerol-plasticized pea protein isolate (PPI) films. OA acidified film-forming solution increased its viscosity more effectively than CW. At...