A5002005176- Gut microbiota and health
- Veterinary Practice and Education Studies
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Eosinophilic Esophagitis
- Clinical Nutrition and Gastroenterology
- IL-33, ST2, and ILC Pathways
- Animal Disease Management and Epidemiology
- Human-Animal Interaction Studies
- Zoonotic diseases and public health
- Inflammatory Bowel Disease
- Microscopic Colitis
- Mycobacterium research and diagnosis
- Digestive system and related health
- Gastrointestinal motility and disorders
- Clinical practice guidelines implementation
- Vector-Borne Animal Diseases
- Eosinophilic Disorders and Syndromes
- Diet and metabolism studies
- Child and Adolescent Health
- Agriculture and Farm Safety
- Nutrition and Health in Aging
- Veterinary Oncology Research
- vaccines and immunoinformatics approaches
- Agriculture Sustainability and Environmental Impact
KU Leuven
2021-2025
Università Cattolica del Sacro Cuore
2024-2025
University of the Sacred Heart
2025
Istituti di Ricovero e Cura a Carattere Scientifico
2024-2025
Agostino Gemelli University Polyclinic
2024-2025
Background Several randomised clinical trials (RCTs) performing faecal microbiota transplantation (FMT) for the management of inflammatory bowel disease (IBD), particularly ulcerative colitis, have recently been published, but with major variations in study design. These include differences administered dose, route and frequency delivery, type placebo evaluated endpoints. Although overall outcomes appear to be promising, they are highly dependent on both donor recipient factors. Objective To...
Short-chain fatty acids as well their bacterial producers are of increasing interest in inflammatory bowel diseases. Although less studied compared to butyrate, acetate might also be it may toxic epithelial cells, stimulate butyrate-producing bacteria by cross-feeding, and have anti-inflammatory barrier-protective properties. Moreover, one the causative factors probiotic potency Saccharomyces cerevisae var. boulardii is thought its high production. Therefore, objective was preclinically...
Rigorous donor preselection on microbiota level, strict anaerobic processing, and repeated fecal transplantation (FMT) administration were hypothesized to improve FMT induction of remission in ulcerative colitis (UC). The RESTORE-UC trial was a multi-centric, double-blind, sham-controlled, randomized trial. Patients with moderate severe UC (defined by total Mayo 4-10) randomly allocated receive 4 anaerobic-prepared allogenic or autologous FMTs. Allogenic material selected after rigorous...
Abstract Background Fecal microbiota transplantation (FMT) is emerging as a potential treatment modality for individuals living with inflammatory bowel disease (IBD). Despite its promise, the effectiveness of FMT treating IBD, particularly ulcerative colitis (UC), still requires thorough clinical investigation. Notwithstanding differences in methodologies, current studies demonstrate inducing remission UC patients. Therefore, standardized and robust randomized trials (RCTs) are needed to...
Abstract Background Short bowel syndrome (SBS) is a malabsorptive condition mostly caused by massive surgical resection of the small intestine and associated with significant morbidity mortality, reduced quality life, high healthcare costs1. Crohn’s disease (CD) represents potential cause leading to an increased risk SBS considerable clinical consequences2. Human microbiota-associated (HMA) mice represent model establish human fecal microbiota in preclinical models3-4. Here, for first time,...
Abstract Background Sarcopenia is a syndrome characterized by progressive and generalized loss of skeletal muscle mass. Primary sarcopenia associated with aging, while secondary caused various factors, including inflammatory conditions such as bowel disease (IBD) [1]. The complex interaction between inflammation, intestinal dysbiosis, malnutrition in IBD patients referred to the gut-muscle axis [2]. High levels cytokines dysbiosis activate pathways that disrupt protein synthesis [3]....
Acetate-producing Saccharomyces cerevisiae var. boulardii strains could exert improved effects on ulcerative colitis, which here, was preclinically evaluated in an acute dextran sodium sulphate induced model of colitis. Nine-week-old female mice were divided into 12 groups, receiving either drinking water or 2.75% for 7 days, combined with a daily gavage various treatments different levels acetate accumulation: sham control (phosphate buffered saline, no acetate), non-probiotic (Baker’s...
Abstract Background Rigorous donor preselection on microbiota level, strict anaerobic processing, and repeated FMT administration were hypothesized to improve outcomes for induction of remission in UC the RESTORE-UC trial, which we here report clinical results observed microbial changes. Methods The trial was a multi-centric double-blind, sham-controlled randomized trial. Patients with moderate severe (total Mayo 4-10, endoscopic subscore ≥2) randomly allocated receive 4 weekly...
has been a subject of growing interest due to its potential as probiotic microorganism with applications in gastrointestinal health, but the molecular cause for potency remained elusive. The recent discovery that
Eosinophils were previously reported to play a role in intestinal inflammation and fibrosis. Whether this is as bystander or an active participant still up for debate. Moreover, data describing causal relationship between eosinophils fibrosis are scarce. We here aimed elucidate the of pathogenesis Therefore, we stimulated fibroblasts with (active) Eosinophil Cationic Protein (ECP), assessed fibroblast activation via flow cytometry immunocytochemistry. observed decreased when co-cultured...
Abstract Background Four randomized controlled trials studying faecal microbiota transplantation (FMT) in active UC patients showed variable success rates. The efficacy of FMT appears to be influenced by various factors including donor- and procedure-specific characteristics. We hypothesized that the outcome with could improved donor preselection on level, using a strict anaerobic approach, repeated administration. Methods RESTORE-UC trial (NCT03110289) was national, multi-centric...
Abstract Background In several disease areas, such as in the liver and lungs, a pro-fibrotic role for eosinophils has been suggested. However, information about of development intestinal fibrosis is scarce. We therefore aimed to assess functional mechanistic fibroblast activation. Methods Chronic DSS colitis (3 cycles 1 week administration followed by 2 weeks recovery (1.75% - 2.25% 2.25%)) was introduced 6-8-week-old C57BL/6 RAG-/- mice induce (Figure 1A). Mice were injected...
Abstract Background The probiotic Saccharomyces cerevisiae var. boulardii (Sb) may be optimized for therapeutic use in ulcerative colitis by enhancing its acetate production, as is a key factor efficacy and was shown to have anti-inflammatory barrier-protective effects vitro (Deleu, 2023). Therefore, higher producing Sb strains been engineered which were preclinically evaluated an acute DSS mice model of colitis. Methods Nine-week-old female C57/Bl6 (N=120) allocated 12 treatment groups...
Abstract Background and Aims The gut microbiome diet have been shown to be associated with IBD pathogenesis, implicated as triggers in disease development. Increasing evidence shows that dysbiosis Western diets may drive sustain intestinal inflammation. Therefore, faecal microbiota transplantation (FMT) has hypothesized restore a balanced microbiome, leading reduced inflammation improved function. mechanisms by which FMT exerts its therapeutic effects are not fully understood. Yet, promising...
Background: Eosinophils were previously reported to play a role in intestinal inflammation and fibrosis. Whether this is as bystander or an active participant still up for debate. Moreover, data describing causal relationship between eosinophils fibrosis are scarce. Therefore, we aimed elucidate the of pathogenesis fibrosis.Methods: In that context, stimulated fibroblasts with Eosinophil Cationic Protein assessed fibroblast activation via flow cytometry immunocytochemistry. Furthermore,...
A role for eosinophils in intestinal inflammation and fibrosis the context of inflammatory bowel disease has been suggested, yet precise nature, whether causal or secondary remains debated. Hence, it unclear targeting should be further explored as a treatment option disease.
Abstract Background Eosinophils might play a pro-inflammatory role in inflammatory bowel disease (IBD). However, the of eosinophils intestinal fibrosis remains poorly understood, although pro-fibrotic function has been hypothesized based on data outside gastrointestinal tract. Therefore, we aimed to unravel chronic inflammation and explore eosinophil depletion as potential therapeutic target. Methods A 3-cycles dextran sodium sulphate (DSS) model was induced 6-8-week-old C57BL/6 wild type...
Abstract Background It has been hypothesized that the probiotic potential of yeast Saccharomyces boulardii (Sb) is associated with its acetate production. This SCFA interest in IBD due to cross-feeding beneficial butyrate-producing bacteria and low toxicity epithelial cells . Our previous vitro work supports this hypothesis (Deleu et al, 2022) but still requires vivo validation. Therefore, we evaluated effect different engineered Sb strains producing variable amounts DSS-induced colitis...
Abstract Background Short-chain fatty acids (SCFA), such as acetate and butyrate, have gained increasing interest for their potential to suppress IBD inflammation1,4. Most data been generated showing beneficial effects on the gut microbiome composition, intestinal barrier function immune system1. The of are less known, despite its lower toxicity epithelial cells ability support growth butyrate-producing bacteria by metabolic cross-feeding2. Furthermore, probiotic effect biotherapeutic yeast...