A5010804491- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Corneal Surgery and Treatments
- Hydrogels: synthesis, properties, applications
- Bone Tissue Engineering Materials
- Innovative Teaching Methods
- Advanced biosensing and bioanalysis techniques
- Ultrasound and Hyperthermia Applications
- Nanoparticle-Based Drug Delivery
- Engineering Education and Pedagogy
- Spectroscopy and Quantum Chemical Studies
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Ultrasound and Cavitation Phenomena
- Nanomaterials for catalytic reactions
- Periodontal Regeneration and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Pluripotent Stem Cells Research
- Tendon Structure and Treatment
- Advanced Thermodynamics and Statistical Mechanics
Pennsylvania State University
2019-2025
University of Oklahoma
2012-2020
Abstract Current nucleic acid delivery methods have not achieved efficient, non‐toxic of miRNAs with tumor‐specific selectivity. In this study, a new system based on light‐inducible gold–silver–gold, core–shell–shell (CSS) nanoparticles is presented. This delivers small therapeutics precise spatiotemporal control, demonstrating the potential for achieving selectivity and efficient miRNA mimics. The particles leverage photothermal heating metal due to local surface plasmonic resonance...
The human amniotic membrane (hAM) is a collagen-based extracellular matrix derived from the placenta. It readily available, inexpensive, and naturally biocompatible material. Over past decade, development of tissue engineering regenerative medicine, along with new decellularization protocols, has recast this simple biomaterial as tunable for cellularized engineered constructs. Thanks to its biocompatibility, decellularized hAM now commonly used in broad range medical fields. New preparation...
The development of tunable, ultrasound-responsive hydrogels that can deliver protein payload on-demand when exposed to focused ultrasound is described in this study. Reversible Diels-Alder linkers, which undergo a retro reaction stimulated with ultrasound, were used cross-link chitosan entrapped FITC-BSA as model therapeutic payload. Two linkage compositions large differences the reverse energy barriers compared explore influence linker composition on response. Selected physicochemical...
An ultrasound-responsive hydrogel system was developed that provides on-demand release when stimulated by focused ultrasound (fUS). Diels–Alder cycloadducts crosslinked polyethylene glycol (PEG) hydrogels and underwent a retrograde reaction exposed to fUS. Four-arm eight-arm furan-based compositions were used evaluate the link between crosslinking density fUS-induced retention rates. PEG with glutaraldehyde also as non-Diels–Alder control hydrogel. By increasing exposure time amplitude of...
The mechanical properties of soft materials are critically important for a wide range applications ranging from packaging to biomedical purposes. We have constructed simple testing apparatus using off-the-shelf and open-source software total cost less than $100. device consists wooden frame supporting central loading attached via drawer slides. To perform test, sample is secured within two custom-made 3D-printed clamps affixed brackets on the base load cell. extension force applied by user...
It is becoming more apparent in tissue engineering applications that fine temporal control of multiple therapeutics desirable to modulate progenitor cell fate and function. Herein, the independent co-delivery miR-148b miR-21 mimic plasmonic nanoparticle conjugates induce osteogenic differentiation human adipose stem cells (hASCs), a de novo fashion, described. By applying thermally labile retro-Diels-Alder caging linkage chemistry, these miRNAs can be triggered de-cage serially with discrete...
Abstract Cardiovascular mechanical stresses trigger physiological and pathological cellular reactions including secretion of Transforming Growth Factor β1 ubiquitously in a latent form (LTGF-β1). While complex shear can activate LTGF-β1, the mechanisms underlying LTGF-β1 activation remain unclear. We hypothesized that different types stress differentially LTGF-β1. designed custom-built cone-and-plate device to generate steady (SS) forces, which are physiologic, or oscillatory (OSS) forces...
In this study, we describe the development of an alternating magnetic field radiofrequency (AMF-RF) mediated siRNA delivery platform for intracellular release miRNA mimic in A549 cells. Iron oxide nanoparticles were synthesized with discrete chemical attachment sites conjugation payloads. The was controlled by a thermally labile Diels-Alder linker, tethering oligonucleotide to surface inactive state. Diels Alder linker acted as effective control switch spatiotemporally deliver therapeutic...
This study describes the development of an ultrasound-responsive polymer system that provides on-demand degradation when exposed to high-intensity focused ultrasound (HIFU). Diels-Alder cycloadducts were used crosslink polycaprolactone (PCL) polymers and underwent a retro reaction stimulated with HIFU. Two compositions explored evaluate link between reverse energy barriers rates. PCL crosslinked isosorbide was also as non-Diels-Alder-based control polymer. An increase HIFU exposure time...
In this study, we utilized selectively modified, biodegradable polymer-based polyplexes to deliver custom, exogenous miR-148b mimics induce apoptosis in human lung cancer (A549) cells. The gene regulatory effects of the payload miRNA (miR-148b-3p) were first evaluated through bioinformatic analyses uncover specific targets involved critical carcinogenic pathways. Hyperbranched poly(β amino ester) (hPBAE) loaded with custom then developed for targeted therapy. When
Investigation into the use of thermally reversible Diels-Alder chemistry coupled with magnetic iron oxide nanoparticles has grown over last decade. This technology been used for a variety applications such as thermoresponsive materials, catalytic chemistry, and drug delivery systems. In this study, we evaluate two distinct labile linkers release payloads from surface nanoparticles. Density functional theory (DFT) computational predictions Gibbs free energy enthalpy reaction barriers were...
There exists a clear need for alternative sources of small-diameter vascular grafts treating the millions patients who suffer from cardiovascular disease each year. Bypass surgery or replacement defective vessels is often required to treat coronary heart disease, but there limited supply suitable autologous grafts, and synthetic are ineffective vessels. Inherent thrombogenicity, compliance mismatch, patency rates all complications with current options. Tissue engineering has potential...
The objective of this study is to fabricate a small-diameter tissue engineered blood vessel (TEBV) by using human adipose-derived stem cells (ADSC) in combination with the amniotic membrane as mechanically-sound biological substrate. Our technology begins differentiating into smooth muscle (SMC) and seeding them onto flat sheet membrane. We assessed our hypothesis that several types SMC can successfully attach proliferate on fluorescently staining cell nuclei DAPI characteristic actin...
Abstract Commercially available mechanical testing devices for characterization of biomaterials can cost tens thousands dollars. In order to accommodate laboratory courses with several students, access multiple enhance the student experience by allowing students have most hands-on time equipment. This be prohibitively expensive, however. To address this problem, custom made using open-source hardware and software systems such as Arduino built materials costing a fraction commercially...