Significance High intensity focused ultrasound (HIFU) therapy is a promising, clinically adopted method of noninvasive tissue ablation used to treat both benign and malignant conditions. This work presents, our knowledge, the first in vivo validation previously developed HIFU-based that allows for fractionation targeted into subcellular debris—boiling histotripsy—in large animal model. While fractionating soft tissue, boiling histotripsy shown spare adjacent connective structures such as...

Atomization and fountain formation is a well-known phenomenon that occurs when focused ultrasound wave in liquid encounters an air interface. High intensity (HIFU) has been shown to fractionate tissue into submicron-sized fragments process termed boiling histotripsy, wherein the superheats at focus, producing millimetre-sized or vapour bubble several milliseconds. Yet question of how this creates remains. The hypothesis work can behave as such it atomizes forms within produced histotripsy....

When focused ultrasound waves of moderate intensity in liquid encounter an air interface, a chain drops emerges from the surface to form what is known as drop-chain fountain. Atomization, or emission micro-droplets, occurs when acoustic exceeds liquid-dependent threshold. While cavitation-wave hypothesis, which states that atomization arises combination capillary-wave instabilities and cavitation bubble oscillations, currently most accepted theory atomization, more data on roles cavitation,...

Abstract Boiling histotripsy (BH) is a High Intensity Focused Ultrasound (HIFU) method for precise mechanical disintegration of target tissue using millisecond-long pulses containing shocks. BH treatments with real-time ultrasound (US) guidance allowed by BH-generated bubbles were previously demonstrated ex vivo and in exposed porcine liver small animals. Here, the feasibility US-guided transabdominal partially transcostal ablation kidney an acute swine model was evaluated 6 parameters were:...

The inability to spatiotemporally guide proteins in tissues and efficiently deliver them into cells remains a key barrier realizing their full potential precision medicine. Here, we report ultrasound-sensitive fluoro-protein nanoemulsions which can be acoustically tracked, guided, activated for on-demand cytosolic delivery of proteins, including antibodies, using clinically relevant diagnostic ultrasound. This advance is accessed through the discovery family fluorous tags, or FTags, that...

The development of tunable, ultrasound-responsive hydrogels that can deliver protein payload on-demand when exposed to focused ultrasound is described in this study. Reversible Diels-Alder linkers, which undergo a retro reaction stimulated with ultrasound, were used cross-link chitosan entrapped FITC-BSA as model therapeutic payload. Two linkage compositions large differences the reverse energy barriers compared explore influence linker composition on response. Selected physicochemical...

An ultrasound-responsive hydrogel system was developed that provides on-demand release when stimulated by focused ultrasound (fUS). Diels–Alder cycloadducts crosslinked polyethylene glycol (PEG) hydrogels and underwent a retrograde reaction exposed to fUS. Four-arm eight-arm furan-based compositions were used evaluate the link between crosslinking density fUS-induced retention rates. PEG with glutaraldehyde also as non-Diels–Alder control hydrogel. By increasing exposure time amplitude of...

No AccessJournal of UrologyInvestigative Urology1 Feb 2012Focused Ultrasound to Expel Calculi From the Kidney Anup Shah, Jonathan D. Harper, Bryan W. Cunitz, Yak-Nam Wang, Marla Paun, Julianna C. Simon, Wei Lu, Peter J. Kaczkowski, and Michael R. Bailey ShahAnup Shah More articles by this author , HarperJonathan Harper CunitzBryan Cunitz WangYak-Nam Wang PaunMarla Paun SimonJulianna Simon LuWei Lu KaczkowskiPeter Kaczkowski BaileyMichael View All Author...

No AccessJournal of UrologyInvestigative Urology1 Sep 2013Focused Ultrasound to Expel Calculi from the Kidney: Safety and Efficacy a Clinical Prototype Device Jonathan D. Harper, Mathew Sorensen, Bryan W. Cunitz, Yak-Nam Wang, Julianna C. Simon, Frank Starr, Marla Paun, Barbrina Dunmire, H. Denny Liggitt, Andrew P. Evan, James A. McAteer, Ryan S. Hsi, Michael R. Bailey HarperJonathan Harper Department Urology, School Medicine, University Washington, Seattle, Washington More articles by this...

The color Doppler ultrasound twinkling artifact, which highlights kidney stones with rapidly changing color, has the potential to improve stone detection; however, its inconsistent appearance limited clinical utility. Recently, it was proposed stable crevice bubbles on surface cause twinkling; hypothesis is not fully accepted because have been directly observed. In this paper, micron or submicron-sized predicted by bubble are enlarged in of five primary compositions exposure acoustic...

Abstract Macrophages are specialized phagocytes that play central roles in immunity and tissue repair. Their diverse functionalities have led to an evolution of new allogenic autologous macrophage products. However, realizing the full therapeutic potential these cell‐based therapies requires development imaging technologies can track immune cell migration within tissues real‐time. Such innovations will not only inform treatment regimens empower interpretation outcomes but also enable...

Focused ultrasonic propulsion is a new noninvasive technique designed to move kidney stones and stone fragments out of the urinary collecting system. However, our knowledge extent tissue injury associated with this not known. We quantitated amount produced by focused under simulated clinical treatment conditions higher power or continuous duty cycles. compared those results extracorporeal shock wave lithotripsy injury.A human calcium oxalate monohydrate and/or nickel beads were implanted...

This study describes the development of an ultrasound-responsive polymer system that provides on-demand degradation when exposed to high-intensity focused ultrasound (HIFU). Diels-Alder cycloadducts were used crosslink polycaprolactone (PCL) polymers and underwent a retro reaction stimulated with HIFU. Two compositions explored evaluate link between reverse energy barriers rates. PCL crosslinked isosorbide was also as non-Diels-Alder-based control polymer. An increase HIFU exposure time...

Abstract Objective —To determine the change in stiffness as evaluated by dorsal bending moment of cervical vertebral specimens obtained from canine cadavers after internally stabilizing motion unit (VMU) C4 and C5 with a traditional pin-polymethylmethacrylate (PMMA) fixation implant or novel screw-bar–PMMA implant. Sample Population —12 column (C3 through C6) cadavers. Procedures —A was applied to before VMU use pin-PMMA Biomechanical data were collected compared within specimen (unaltered...

The global prevalence and incidence of renal calculi is reported to be increasing. Of the patients that undergo surgical intervention, nearly half experience symptomatic complications associated with stone fragments are not passed require follow-up intervention. In a clinical simulation using prototype, ultrasonic propulsion was proven effective at repositioning kidney stones in pigs. use ultrasound reposition smaller or location facilitates spontaneous clearance could therefore improve...

Purpose: In the first-in-human trial of ultrasonic propulsion, subjects passed collections residual stone fragments repositioned with a C5-2 probe. Here, effectiveness and safety in moving multiple are compared between custom (SC-50) probe that produces longer focal beam burst duration. Materials Methods: Effectiveness was quantified by number stones expelled from calyx phantom consisting 30-mm deep, water-filled well block tissue mimicking material. Each positioned below to move against...

In Fall 2022, I offered a new course on research communication within the Graduate Program in Acoustics at Penn State. With 8 students from and Biomedical Engineering, was split into three modules of grant writing, paper outreach. found were reasonably adept critically reading single papers. They also generally understood mechanics writing sentences, although combining sentences paragraphs, connecting ideas, reducing fluff, adding emphasis to key points areas that significantly improved...